WWW.JNEUROSCI.ORG
-
The Journal of Neuroscience MBF Stereo Investigator
QUICK SEARCH:   [advanced]


     
-


HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an eLetter
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (33)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yoshihara, M.
Right arrow Articles by Kidokoro, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yoshihara, M.
Right arrow Articles by Kidokoro, Y.

 Previous Article  |  Next Article 

The Journal of Neuroscience, November 15, 2000, 20(22):8315-8322

Two Independent Pathways Mediated by cAMP and Protein Kinase A Enhance Spontaneous Transmitter Release at Drosophila Neuromuscular Junctions

Motojiro Yoshihara, Kazuhiro Suzuki, and Yoshiaki Kidokoro

Institute for Behavioral Sciences, Gunma University School of Medicine, Maebashi, 371-8511 Japan

cAMP is thought to be involved in learning process and known to enhance transmitter release in various systems. Previously we reported that cAMP enhances spontaneous transmitter release in the absence of extracellular Ca2+ and that the synaptic vesicle protein neuronal-synaptobrevin (n-syb), is required in this enhancement (n-syb-dependent; Yoshihara et al., 1999). In the present study, we examined the cAMP-induced enhancement of transmitter release in the presence of external Ca2+. We raised the intracellular concentration of cAMP by application of either forskolin, an activator of adenylyl cyclase, or by 4-chlorophenylthio-(CPT)-cAMP, a membrane-permeable analog of cAMP, in the presence of external Ca2+, while recording miniature synaptic currents (mSCs) at the neuromuscular junction in n-syb null mutant embryos. The frequency of mSCs increased in response to elevation of cAMP, and this effect of cAMP was completely blocked by Co2+ (n-syb-independent pathway). In contrast, in wild-type embryos the cAMP-induced mSC frequency increase was partially blocked by Co2+. In a mutant, DC0, defective in protein kinase A (PKA), nerve-evoked synaptic currents were indistinguishable from the control, but mSCs were less frequent. In this mutant the enhancement by cAMP of both nerve-evoked and spontaneous transmitter release was completely absent, even in the presence of external Ca2+. Taken together, these results suggest that cAMP enhances spontaneous transmitter release by increasing Ca2+ influx (n-syb-independent) as well as by modulating the release mechanism without Ca2+ influx (n-syb-dependent) in wild-type embryos, and these two effects are mediated by PKA encoded by the DC0 gene.

Key words: cAMP; spontaneous synaptic currents; neuronal-synaptobrevin; DC0; PKA; forskolin; neuromuscular junction; Drosophila; myosin heavy chain mutant

Copyright © 2000 Society for Neuroscience  0270-6474/00/20228315-08$05.00/0


This article has been cited by other articles:


Home page
 J. Neurophysiol.Home page
K. B. Grey and B. D. Burrell
Forskolin Induces NMDA Receptor-Dependent Potentiation at a Central Synapse in the Leech
J Neurophysiol, May 1, 2008; 99(5): 2719 - 2724.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
A. A. Long, E. Kim, H.-T. Leung, E. Woodruff III, L. An, R. W. Doerge, W. L. Pak, and K. Broadie
Presynaptic Calcium Channel Localization and Calcium-Dependent Synaptic Vesicle Exocytosis Regulated by the Fuseless Protein
J. Neurosci., April 2, 2008; 28(14): 3668 - 3682.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
A. Minami, Y.-f. Xia, and R. S. Zucker
Increased Ca2+ influx through Na+/Ca2+ exchanger during long-term facilitation at crayfish neuromuscular junctions
J. Physiol., December 1, 2007; 585(2): 413 - 427.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
Y.-C. Kim, H.-G. Lee, and K.-A. Han
D1 Dopamine Receptor dDA1 Is Required in the Mushroom Body Neurons for Aversive and Appetitive Learning in Drosophila
J. Neurosci., July 18, 2007; 27(29): 7640 - 7647.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
A. Maximov, O.-H. Shin, X. Liu, and T. C. Sudhof
Synaptotagmin-12, a synaptic vesicle phosphoprotein that modulates spontaneous neurotransmitter release
J. Cell Biol., January 1, 2007; 176(1): 113 - 124.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
M. Yoshihara, B. Adolfsen, K. T. Galle, and J. T. Littleton
Retrograde Signaling by Syt 4 Induces Presynaptic Release and Synapse-Specific Growth
Science, November 4, 2005; 310(5749): 858 - 863.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
S. Seino and T. Shibasaki
PKA-Dependent and PKA-Independent Pathways for cAMP-Regulated Exocytosis
Physiol Rev, October 1, 2005; 85(4): 1303 - 1342.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
T. Okamoto, T. Tamura, K. Suzuki, and Y. Kidokoro
External Ca2+ Dependency of Synaptic Transmission in Drosophila synaptotagmin I Mutants
J Neurophysiol, August 1, 2005; 94(2): 1574 - 1586.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. J. Mosca, R. A. Carrillo, B. H. White, and H. Keshishian
Dissection of synaptic excitability phenotypes by using a dominant-negative Shaker K+ channel subunit
PNAS, March 1, 2005; 102(9): 3477 - 3482.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. Rohrbough, E. Rushton, L. Palanker, E. Woodruff, H. J. G. Matthies, U. Acharya, J. K. Acharya, and K. Broadie
Ceramidase Regulates Synaptic Vesicle Exocytosis and Trafficking
J. Neurosci., September 8, 2004; 24(36): 7789 - 7803.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
A. W. Hendricson, J. R. Sibbald, and R. A. Morrisett
Ethanol Alters the Frequency, Amplitude, and Decay Kinetics of Sr2+-Supported, Asynchronous NMDAR mEPSCs in Rat Hippocampal Slices
J Neurophysiol, June 1, 2004; 91(6): 2568 - 2577.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
D. Hou, K. Suzuki, W. J. Wolfgang, C. Clay, M. Forte, and Y. Kidokoro
Presynaptic Impairment of Synaptic Transmission in Drosophila Embryos Lacking Gs{alpha}
J. Neurosci., July 2, 2003; 23(13): 5897 - 5905.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
D. W. Munno, D. J. Prince, and N. I. Syed
Synapse Number and Synaptic Efficacy Are Regulated by Presynaptic cAMP and Protein Kinase A
J. Neurosci., May 15, 2003; 23(10): 4146 - 4155.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
H.-X. Chen and S. N. Roper
PKA and PKC Enhance Excitatory Synaptic Transmission in Human Dentate Gyrus
J Neurophysiol, May 1, 2003; 89(5): 2482 - 2488.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
R. B. Renden and K. Broadie
Mutation and Activation of Galpha s Similarly Alters Pre- and Postsynaptic Mechanisms Modulating Neurotransmission
J Neurophysiol, May 1, 2003; 89(5): 2620 - 2638.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Bhattacharya, B. A. Stewart, B. A. Niemeyer, R. W. Burgess, B. D. McCabe, P. Lin, G. Boulianne, C. J. O'Kane, and T. L. Schwarz
From the Cover: Members of the synaptobrevin/vesicle-associated membrane protein (VAMP) family in Drosophila are functionally interchangeable in vivo for neurotransmitter release and cell viability
PNAS, October 15, 2002; 99(21): 13867 - 13872.
[Abstract] [Full Text] [PDF]


-

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help
-
Copyright 2008 by Society for Neuroscience ONLINE ISSN: 1529-2401
-