QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (53) Citing Articles via Google Scholar Google Scholar Articles by Albillos, A. Articles by Moser, T. Search for Related Content PubMed PubMed Citation Articles by Albillos, A. Articles by Moser, T.

 Previous Article  |  Next Article 

The Journal of Neuroscience, November 15, 2000, 20(22):8323-8330

R-Type Ca²⁺ Channels Are Coupled to the Rapid Component of Secretion in Mouse Adrenal Slice Chromaffin Cells

Almudena Albillos^{1, 2}, Erwin Neher¹, and Tobias Moser^{1, 3}

¹ Department of Membrane Biophysics, Max-Planck Institute for Biophysical Chemistry, 37077 Goettingen, Germany, ² Instituto de Farmacología Teófilo Hernando, Departamento de Farmacología y Terapeútica, Facultad de Medicina, Universidad Autónoma de Madrid, 28029 Madrid, Spain, and ³ Department of Otolaryngology, Goettingen University Medical School, 37073 Goettingen, Germany

Patch-clamp measurements of Ca²⁺ currents and membrane capacitance were performed on slices of mouse adrenal glands, using the perforated-patch configuration of the patch-clamp technique. These recording conditions are much closer to the in vivo situation than those used so far in most electrophysiological studies in adrenal chromaffin cells (isolated cells maintained in culture and whole-cell configuration). We observed profound discrepancies in the quantities of Ca²⁺ channel subtypes (P-, Q-, N-, and L-type Ca²⁺ channels) described for isolated mouse chromaffin cells maintained in culture. Differences with respect to previous studies may be attributable not only to culture conditions, but also to the patch-clamp configuration used. Our experiments revealed the presence of a Ca²⁺ channel subtype never before described in chromaffin cells, a toxin and dihydropyridine-resistant Ca²⁺ channel with fast inactivation kinetics, similar to the R-type Ca²⁺ channel described in neurons. This channel contributes 22% to the total Ca²⁺ current and controls 55% of the rapid secretory response evoked by short depolarizing pulses. Our results indicate that R-type Ca²⁺ channels are in close proximity with the exocytotic machinery to rapidly regulate the secretory process.

Key words: calcium channels; exocytosis; membrane capacitance measurements; adrenal slice; chromaffin cell; calcium-secretion coupling

---

Copyright © 2000 Society for Neuroscience  0270-6474/00/20228323-08$05.00/0

This article has been cited by other articles:

				A. G. Garcia, A. M. Garcia-De-Diego, L. Gandia, R. Borges, and J. Garcia-Sancho Calcium signaling and exocytosis in adrenal chromaffin cells. Physiol Rev, October 1, 2006; 86(4): 1093 - 1131. [Abstract] [Full Text] [PDF]

				G. Arroyo, J. Fuentealba, N. Sevane-Fernandez, M. Aldea, A. G. Garcia, and A. Albillos Amperometric Study of the Kinetics of Exocytosis in Mouse Adrenal Slice Chromaffin Cells: Physiological and Methodological Insights J Neurophysiol, September 1, 2006; 96(3): 1196 - 1202. [Abstract] [Full Text] [PDF]

				A. Giancippoli, M. Novara, A. de Luca, P. Baldelli, A. Marcantoni, E. Carbone, and V. Carabelli Low-Threshold Exocytosis Induced by cAMP-Recruited CaV3.2 ({alpha}1H) Channels in Rat Chromaffin Cells Biophys. J., March 1, 2006; 90(5): 1830 - 1841. [Abstract] [Full Text] [PDF]

				J. Xu, K. S. Tang, V. B. Lu, C. P. Weerasinghe, A. Tse, and F. W. Tse Maintenance of quantal size and immediately releasable granules in rat chromaffin cells by glucocorticoid Am J Physiol Cell Physiol, November 1, 2005; 289(5): C1122 - C1133. [Abstract] [Full Text] [PDF]

				A. Rocher, E. Geijo-Barrientos, A. I. Caceres, R. Rigual, C. Gonzalez, and L. Almaraz Role of voltage-dependent calcium channels in stimulus-secretion coupling in rabbit carotid body chemoreceptor cells J. Physiol., January 15, 2005; 562(2): 407 - 420. [Abstract] [Full Text] [PDF]

				K. Haneda and Y. Oka Selective Modulation of Voltage-Gated Calcium Channels in the Terminal Nerve Gonadotropin-Releasing Hormone Neurons of a Teleost, the Dwarf Gourami (Colisa lalia) Endocrinology, October 1, 2004; 145(10): 4489 - 4499. [Abstract] [Full Text] [PDF]

				M. Novara, P. Baldelli, D. Cavallari, V. Carabelli, A. Giancippoli, and E. Carbone Exposure to cAMP and {beta}-adrenergic stimulation recruits CaV3 T-type channels in rat chromaffin cells through Epac cAMP-receptor proteins J. Physiol., July 15, 2004; 558(2): 433 - 449. [Abstract] [Full Text] [PDF]

				M. Watanabe, Y. Sakuma, and M. Kato High Expression of the R-Type Voltage-Gated Ca2+ Channel and Its Involvement in Ca2+-Dependent Gonadotropin-Releasing Hormone Release in GT1-7 Cells Endocrinology, May 1, 2004; 145(5): 2375 - 2383. [Abstract] [Full Text] [PDF]

				R. A. Bannister, K. Melliti, and B. A. Adams Differential Modulation of CaV2.3 Ca2+ Channels by G{alpha}q/11-Coupled Muscarinic Receptors Mol. Pharmacol., February 1, 2004; 65(2): 381 - 388. [Abstract] [Full Text] [PDF]

				M. Kato, K. Ui-Tei, M. Watanabe, and Y. Sakuma Characterization of Voltage-Gated Calcium Currents in Gonadotropin-Releasing Hormone Neurons Tagged with Green Fluorescent Protein in Rats Endocrinology, November 1, 2003; 144(11): 5118 - 5125. [Abstract] [Full Text] [PDF]

				A. Pereverzev, M. Mikhna, R. Vajna, C. Gissel, M. Henry, M. Weiergraber, J. Hescheler, N. Smyth, and T. Schneider Disturbances in Glucose-Tolerance, Insulin-Release, and Stress-Induced Hyperglycemia upon Disruption of the Cav2.3 ({alpha}1E) Subunit of Voltage-Gated Ca2+ Channels Mol. Endocrinol., April 1, 2002; 16(4): 884 - 895. [Abstract] [Full Text] [PDF]

				O. Wiser, R. Cohen, and D. Atlas Ionic dependence of Ca2+ channel modulation by syntaxin 1A PNAS, March 19, 2002; 99(6): 3968 - 3973. [Abstract] [Full Text] [PDF]

				S.-C. Lee, S. Choi, T. Lee, H.-L. Kim, H. Chin, and H.-S. Shin Molecular basis of R-type calcium channels in central amygdala neurons of the mouse PNAS, February 14, 2002; (2002) 52697799. [Abstract] [Full Text] [PDF]

				S. Gasparini, A. M. Kasyanov, D. Pietrobon, L. L. Voronin, and E. Cherubini Presynaptic R-Type Calcium Channels Contribute to Fast Excitatory Synaptic Transmission in the Rat Hippocampus J. Neurosci., November 15, 2001; 21(22): 8715 - 8721. [Abstract] [Full Text] [PDF]

				M. F. Cano-Abad, M. Villarroya, A. G. Garcia, N. H. Gabilan, and M. G. Lopez Calcium Entry through L-type Calcium Channels Causes Mitochondrial Disruption and Chromaffin Cell Death J. Biol. Chem., October 19, 2001; 276(43): 39695 - 39704. [Abstract] [Full Text] [PDF]

				A. O. Martin, M.-N. Mathieu, C. Chevillard, and N. C. Guerineau Gap Junctions Mediate Electrical Signaling and Ensuing Cytosolic Ca2+ Increases between Chromaffin Cells in Adrenal Slices: A Role in Catecholamine Release J. Neurosci., August 1, 2001; 21(15): 5397 - 5405. [Abstract] [Full Text] [PDF]

				S.-C. Lee, S. Choi, T. Lee, H.-L. Kim, H. Chin, and H.-S. Shin Molecular basis of R-type calcium channels in central amygdala neurons of the mouse PNAS, March 5, 2002; 99(5): 3276 - 3281. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help