 |
||||
|
||||
|
||||
|
||||
Previous Article | Next Article
The Journal of Neuroscience, November 15, 2000, 20(22):8390-8400
Cryptic Peripheral Ribosomal Domains Distributed Intermittently along Mammalian Myelinated Axons
Edward Koenig1, Rainer Martin2, Margaret Titmus1, and José R. Sotelo-Silveira3 1 Department of Physiology and Biophysics, University at Buffalo School of Medicine, Buffalo, New York 14214, 2 Universität Ulm, Sektion Elektronenmikroskopie, D-89081 Ulm, Germany, and 3 Biofísica, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay
A growing body of metabolic and molecular evidence of an endogenous protein-synthesizing machinery in the mature axon is a challenge to the prevailing dogma that the latter is dependent exclusively on slow axoplasmic transport to maintain protein mass in a steady state. However, evidence for a systematic occurrence of ribosomes in mature vertebrate axons has been lacking until recently, when restricted ribosomal domains, called "periaxoplasmic plaques," were described in goldfish CNS myelinated axons. Comparable restricted RNA/ribosomal "plaque" domains now have been identified in myelinated axons of lumbar spinal nerve roots in rabbit and rat on the basis of RNase sensitivity of YOYO-1-binding fluorescence, immunofluorescence of ribosome-specific antibodies, and ribosome phosphorus mapping by electron spectroscopic imaging (ESI). The findings were derived from examination of the axoplasm isolated from myelinated fibers as axoplasmic whole mounts and delipidated spinal nerve roots. Ribosomal periaxoplasmic plaque domains in rabbit axons were typically narrow (~2 µm), elongated (~10 µm) sites that frequently were marked by a protruding structure. The domain complexity included an apparent ribosome-binding matrix. The small size, random distribution, and variable intermittent axial spacing of plaques around the periphery of axoplasm near the axon-myelin border are likely reasons why their systematic occurrence has remained undetected in ensheathed axons. The periodic but regular incidence of ribosomal domains provides a structural basis for previous metabolic evidence of protein synthesis in myelinated axons.
Key words: axoplasm; myelinated axons; ribosomes; RNA; YOYO-1; electron spectroscopic imaging; ESI; spinal nerves
Copyright © 2000 Society for Neuroscience 0270-6474/00/20228390-11$05.00/0
This article has been cited by other articles:
F. A. Court, W. T. J. Hendriks, H. D. MacGillavry, J. Alvarez, and J. van Minnen
Schwann Cell to Axon Transfer of Ribosomes: Toward a Novel Understanding of the Role of Glia in the Nervous System
J. Neurosci., October 22, 2008; 28(43): 11024 - 11029.
[Abstract] [Full Text] [PDF]
A. Giuditta, J. Tai Chun, M. Eyman, C. Cefaliello, A. P. Bruno, and M. Crispino
Local Gene Expression in Axons and Nerve Endings: The Glia-Neuron Unit
Physiol Rev, April 1, 2008; 88(2): 515 - 555.
[Abstract] [Full Text] [PDF]
A. Sexton, M. McDonald, C. Cayla, C. Thiemermann, and A. Ahluwalia
12-Lipoxygenase-derived eicosanoids protect against myocardial ischemia/reperfusion injury via activation of neuronal TRPV1
FASEB J, September 1, 2007; 21(11): 2695 - 2703.
[Abstract] [Full Text] [PDF]
D. Willis, K. W. Li, J.-Q. Zheng, J. H. Chang, A. Smit, T. Kelly, T. T. Merianda, J. Sylvester, J. van Minnen, and J. L. Twiss
Differential Transport and Local Translation of Cytoskeletal, Injury-Response, and Neurodegeneration Protein mRNAs in Axons
J. Neurosci., January 26, 2005; 25(4): 778 - 791.
[Abstract] [Full Text] [PDF]
C. Li, Y. Sasaki, K. Takei, H. Yamamoto, M. Shouji, Y. Sugiyama, T. Kawakami, F. Nakamura, T. Yagi, T. Ohshima, et al.
Correlation between Semaphorin3A-Induced Facilitation of Axonal Transport and Local Activation of a Translation Initiation Factor Eukaryotic Translation Initiation Factor 4E
J. Neurosci., July 7, 2004; 24(27): 6161 - 6170.
[Abstract] [Full Text] [PDF]
S.-K. Lee and P. J. Hollenbeck
Organization and translation of mRNA in sympathetic axons
J. Cell Sci., November 1, 2003; 116(21): 4467 - 4478.
[Abstract] [Full Text] [PDF]
J. L. Goldberg
How does an axon grow?
Genes & Dev., April 15, 2003; 17(8): 941 - 958.
[Full Text] [PDF]
I. A. Muslimov, M. Titmus, E. Koenig, and H. Tiedge
Transport of Neuronal BC1 RNA in Mauthner Axons
J. Neurosci., June 1, 2002; 22(11): 4293 - 4301.
[Abstract] [Full Text] [PDF]
M. M. Rolls, D. H. Hall, M. Victor, E. H. K. Stelzer, and T. A. Rapoport
Targeting of Rough Endoplasmic Reticulum Membrane Proteins and Ribosomes in Invertebrate Neurons
Mol. Biol. Cell, May 1, 2002; 13(5): 1778 - 1791.
[Abstract] [Full Text] [PDF]
S. Aronov, G. Aranda, L. Behar, and I. Ginzburg
Visualization of translated tau protein in the axons of neuronal P19 cells and characterization of tau RNP granules
J. Cell Sci., January 10, 2002; 115(19): 3817 - 3827.
[Abstract] [Full Text] [PDF]
J.-Q. Zheng, T. K. Kelly, B. Chang, S. Ryazantsev, A. K. Rajasekaran, K. C. Martin, and J. L. Twiss
A Functional Role for Intra-Axonal Protein Synthesis during Axonal Regeneration from Adult Sensory Neurons
J. Neurosci., December 1, 2001; 21(23): 9291 - 9303.
[Abstract] [Full Text] [PDF]
Y.-N. Jan and L. Y. Jan
Dendrites
Genes & Dev., October 15, 2001; 15(20): 2627 - 2641.
[Full Text] [PDF]
S. Aronov, G. Aranda, L. Behar, and I. Ginzburg
Axonal Tau mRNA Localization Coincides with Tau Protein in Living Neuronal Cells and Depends on Axonal Targeting Signal
J. Neurosci., September 1, 2001; 21(17): 6577 - 6587.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|