QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (72) Citing Articles via Google Scholar Google Scholar Articles by Wilson, S. M. Articles by Miller, R. J. Search for Related Content PubMed PubMed Citation Articles by Wilson, S. M. Articles by Miller, R. J.

 Previous Article  |  Next Article 

The Journal of Neuroscience, December 1, 2000, 20(23):8566-8571

The Status of Voltage-Dependent Calcium Channels in _1E Knock-Out Mice

Scott M. Wilson¹, Peter T. Toth², Seog Bae Oh², Samantha E. Gillard³, Steven Volsen⁴, Dongjun Ren², Louis H. Philipson², E. Chiang Lee¹, Colin F. Fletcher¹, Lino Tessarollo¹, Neal G. Copeland¹, Nancy A. Jenkins¹, and Richard J. Miller²

¹ Mouse Cancer Genetics Program, National Cancer Institute-Frederick Cancer Research and Development Center, Frederick, Maryland 21702, ² Department of Neurobiology, Pharmacology, and Physiology, The University of Chicago, Chicago, Illinois 60637, ³ Eli Lilly and Company, Lilly Corporate Center, Indianapolis, Indiana 46285, and ⁴ Eli Lilly and Company, Erl Wood, Manor, Windlesham, Surrey GU20 6PH, United Kingdom

It has been hypothesized that R-type Ca currents result from the expression of the _1E gene. To test this hypothesis we examined the properties of voltage-dependent Ca channels in mice in which the _1E Ca channel subunit had been deleted. Application of -conotoxin GVIA, -agatoxin IVA, and nimodipine to cultured cerebellar granule neurons from wild-type mice inhibited components of the whole-cell Ba current, leaving a "residual" R current with an amplitude of ~30% of the total Ba current. A minor portion of this R current was inhibited by the _1E-selective toxin SNX-482, indicating that it resulted from the expression of _1E. However, the majority of the R current was not inhibited by SNX-482. The SNX-482-sensitive portion of the granule cell R current was absent from _1E knock-out mice. We also identified a subpopulation of dorsal root ganglion (DRG) neurons from wild-type mice that expressed an SNX-482-sensitive component of the R current. However as with granule cells, most of the DRG R current was not blocked by SNX-482. We conclude that there exists a component of the R current that results from the expression of the _1E Ca channel subunit but that the majority of R currents must result from the expression of other Ca channel  subunits.

Key words: dorsal root ganglia; cerebellar granule cells; pain; synaptic transmission; voltage-dependent calcium channels; _1E knock-out mice

---

Copyright © 2000 Society for Neuroscience  0270-6474/00/20238566-06$05.00/0

This article has been cited by other articles:

				G. Chung, J.N. Rhee, S.J. Jung, J.S. Kim, and S.B. Oh Modulation of CaV2.3 Calcium Channel Currents by Eugenol J. Dent. Res., February 1, 2008; 87(2): 137 - 141. [Abstract] [Full Text] [PDF]

				Y. Yaari, C. Yue, and H. Su Recruitment of apical dendritic T-type Ca2+ channels by backpropagating spikes underlies de novo intrinsic bursting in hippocampal epileptogenesis J. Physiol., April 15, 2007; 580(2): 435 - 450. [Abstract] [Full Text] [PDF]

				Z. Fang, C.-K. Park, H. Y. Li, H. Y. Kim, S.-H. Park, S. J. Jung, J. S. Kim, A. Monteil, S. B. Oh, and R. J. Miller Molecular Basis of Cav2.3 Calcium Channels in Rat Nociceptive Neurons J. Biol. Chem., February 16, 2007; 282(7): 4757 - 4764. [Abstract] [Full Text] [PDF]

				C. Tai, J. B. Kuzmiski, and B. A. MacVicar Muscarinic enhancement of R-type calcium currents in hippocampal CA1 pyramidal neurons. J. Neurosci., June 7, 2006; 26(23): 6249 - 6258. [Abstract] [Full Text] [PDF]

				S. Kaja, R. C. G. Van de Ven, M. D. Ferrari, R. R. Frants, A. M.J.M. Van den Maagdenberg, and J. J. Plomp Compensatory Contribution of Cav2.3 Channels to Acetylcholine Release at the Neuromuscular Junction of Tottering Mice J Neurophysiol, April 1, 2006; 95(4): 2698 - 2704. [Abstract] [Full Text] [PDF]

				M.H. Lee, K.-Y. Yeon, C.-K. Park, H.-Y. Li, Z. Fang, M.S. Kim, S.-Y. Choi, S.J. Lee, S. Lee, K. Park, et al. Eugenol Inhibits Calcium Currents in Dental Afferent Neurons J. Dent. Res., September 1, 2005; 84(9): 848 - 851. [Abstract] [Full Text] [PDF]

				A. E. Metz, T. Jarsky, M. Martina, and N. Spruston R-Type Calcium Channels Contribute to Afterdepolarization and Bursting in Hippocampal CA1 Pyramidal Neurons J. Neurosci., June 15, 2005; 25(24): 5763 - 5773. [Abstract] [Full Text] [PDF]

				M. J. Tyska, A. T. Mackey, J.-D. Huang, N. G. Copeland, N. A. Jenkins, and M. S. Mooseker Myosin-1a Is Critical for Normal Brush Border Structure and Composition Mol. Biol. Cell, May 1, 2005; 16(5): 2443 - 2457. [Abstract] [Full Text] [PDF]

				P. A. Olson, T. Tkatch, S. Hernandez-Lopez, S. Ulrich, E. Ilijic, E. Mugnaini, H. Zhang, I. Bezprozvanny, and D. J. Surmeier G-Protein-Coupled Receptor Modulation of Striatal CaV1.3 L-Type Ca2+ Channels Is Dependent on a Shank-Binding Domain J. Neurosci., February 2, 2005; 25(5): 1050 - 1062. [Abstract] [Full Text] [PDF]

				A. Rocher, E. Geijo-Barrientos, A. I. Caceres, R. Rigual, C. Gonzalez, and L. Almaraz Role of voltage-dependent calcium channels in stimulus-secretion coupling in rabbit carotid body chemoreceptor cells J. Physiol., January 15, 2005; 562(2): 407 - 420. [Abstract] [Full Text] [PDF]

				P. Isope and T. H. Murphy Low threshold calcium currents in rat cerebellar Purkinje cell dendritic spines are mediated by T-type calcium channels J. Physiol., January 1, 2005; 562(1): 257 - 269. [Abstract] [Full Text] [PDF]

				D. Lipscombe, T. D. Helton, and W. Xu L-Type Calcium Channels: The Low Down J Neurophysiol, November 1, 2004; 92(5): 2633 - 2641. [Abstract] [Full Text] [PDF]

				R. A. Bannister, K. Melliti, and B. A. Adams Differential Modulation of CaV2.3 Ca2+ Channels by G{alpha}q/11-Coupled Muscarinic Receptors Mol. Pharmacol., February 1, 2004; 65(2): 381 - 388. [Abstract] [Full Text] [PDF]

				J. Breustedt, K. E. Vogt, R. J. Miller, R. A. Nicoll, and D. Schmitz {alpha}1E-Containing Ca2+ channels are involved in synaptic plasticity PNAS, October 14, 2003; 100(21): 12450 - 12455. [Abstract] [Full Text] [PDF]

				N. T. Blair and B. P. Bean Roles of Tetrodotoxin (TTX)-Sensitive Na+ Current, TTX-Resistant Na+ Current, and Ca2+ Current in the Action Potentials of Nociceptive Sensory Neurons J. Neurosci., December 1, 2002; 22(23): 10277 - 10290. [Abstract] [Full Text] [PDF]

				A. Pereverzev, M. Mikhna, R. Vajna, C. Gissel, M. Henry, M. Weiergraber, J. Hescheler, N. Smyth, and T. Schneider Disturbances in Glucose-Tolerance, Insulin-Release, and Stress-Induced Hyperglycemia upon Disruption of the Cav2.3 ({alpha}1E) Subunit of Voltage-Gated Ca2+ Channels Mol. Endocrinol., April 1, 2002; 16(4): 884 - 895. [Abstract] [Full Text] [PDF]

				S.-C. Lee, S. Choi, T. Lee, H.-L. Kim, H. Chin, and H.-S. Shin Molecular basis of R-type calcium channels in central amygdala neurons of the mouse PNAS, February 14, 2002; (2002) 52697799. [Abstract] [Full Text] [PDF]

				S. Gasparini, A. M. Kasyanov, D. Pietrobon, L. L. Voronin, and E. Cherubini Presynaptic R-Type Calcium Channels Contribute to Fast Excitatory Synaptic Transmission in the Rat Hippocampus J. Neurosci., November 15, 2001; 21(22): 8715 - 8721. [Abstract] [Full Text] [PDF]

				H. Liang and K. S. Elmslie Ef-Current Contributes to Whole-Cell Calcium Current in Low Calcium in Frog Sympathetic Neurons J Neurophysiol, September 1, 2001; 86(3): 1156 - 1163. [Abstract] [Full Text] [PDF]

				W. Xu and D. Lipscombe Neuronal CaV1.3{alpha}1 L-Type Channels Activate at Relatively Hyperpolarized Membrane Potentials and Are Incompletely Inhibited by Dihydropyridines J. Neurosci., August 15, 2001; 21(16): 5944 - 5951. [Abstract] [Full Text] [PDF]

				W. Li, C. Thaler, and P. Brehm Calcium Channels in Xenopus Spinal Neurons Differ in Somas and Presynaptic Terminals J Neurophysiol, July 1, 2001; 86(1): 269 - 279. [Abstract] [Full Text] [PDF]

				S.-C. Lee, S. Choi, T. Lee, H.-L. Kim, H. Chin, and H.-S. Shin Molecular basis of R-type calcium channels in central amygdala neurons of the mouse PNAS, March 5, 2002; 99(5): 3276 - 3281. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help