QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (31) Citing Articles via Google Scholar Google Scholar Articles by Waggoner, L. E. Articles by Schafer, W. R. Search for Related Content PubMed PubMed Citation Articles by Waggoner, L. E. Articles by Schafer, W. R. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB NICOTINE NICOTINE TARTRATE

 Previous Article  |  Next Article 

The Journal of Neuroscience, December 1, 2000, 20(23):8802-8811

Long-Term Nicotine Adaptation in Caenorhabditis elegans Involves PKC-Dependent Changes in Nicotinic Receptor Abundance

Laura E. Waggoner¹, Kari A. Dickinson¹, Daniel S. Poole¹, Yo Tabuse², Johji Miwa², and William R. Schafer¹

¹ Department of Biology, University of California, San Diego, La Jolla, California 92093-0349, and ² Fundamental Research Laboratories, NEC Corporation, Tsukuba 305, Japan

Chronic exposure to nicotine leads to long-term changes in both the abundance and activity of nicotinic acetylcholine receptors, processes thought to contribute to nicotine addiction. We have found that in Caenorhabditis elegans, prolonged nicotine treatment results in a long-lasting decrease in the abundance of nicotinic receptors that control egg-laying. In naive animals, acute exposure to cholinergic agonists led to the efficient stimulation of egg-laying, a response mediated by a nicotinic receptor functionally expressed in the vulval muscle cells. Overnight exposure to nicotine led to a specific and long-lasting change in egg-laying behavior, which rendered the nicotine-adapted animals insensitive to simulation of egg-laying by the nicotinic agonist and was accompanied by a promoter-independent reduction in receptor protein levels. Mutants defective in the gene tpa-1, which encodes a homolog of protein kinase C (PKC), failed to undergo adaptation to nicotine; after chronic nicotine exposure they remained sensitive to cholinergic agonists and retained high levels of receptor protein in the vulval muscles. These results suggest that PKC-dependent signaling pathways may promote nicotine adaptation via regulation of nicotinic receptor synthesis or degradation.

Key words: nicotinic; acetylcholine; Caenorhabditis elegans; protein kinase C; adaptation; levamisole

---

Copyright © 2000 Society for Neuroscience  0270-6474/00/20238802-10$05.00/0

This article has been cited by other articles:

				A. Shteingauz, E. Cohen, Y. Biala, and M. Treinin The BTB-MATH protein BATH-42 interacts with RIC-3 to regulate maturation of nicotinic acetylcholine receptors J. Cell Sci., March 15, 2009; 122(6): 807 - 812. [Abstract] [Full Text] [PDF]

				L. Carnell, J. Illi, S. W. Hong, and S. L. McIntire The G-Protein-Coupled Serotonin Receptor SER-1 Regulates Egg Laying and Male Mating Behaviors in Caenorhabditis elegans J. Neurosci., November 16, 2005; 25(46): 10671 - 10681. [Abstract] [Full Text] [PDF]

				D. Touroutine, R. M. Fox, S. E. Von Stetina, A. Burdina, D. M. Miller III, and J. E. Richmond acr-16 Encodes an Essential Subunit of the Levamisole-resistant Nicotinic Receptor at the Caenorhabditis elegans Neuromuscular Junction J. Biol. Chem., July 22, 2005; 280(29): 27013 - 27021. [Abstract] [Full Text] [PDF]

				S. D. Buckingham, B. Esmaeili, M. Wood, and D. B. Sattelle RNA interference: from model organisms towards therapy for neural and neuromuscular disorders Hum. Mol. Genet., October 1, 2004; 13(suppl_2): R275 - R288. [Abstract] [Full Text] [PDF]

				C. A. Bastiani, S. Gharib, M. I. Simon, and P. W. Sternberg Caenorhabditis elegans G{alpha}q Regulates Egg-Laying Behavior via a PLC{beta}-Independent and Serotonin-Dependent Signaling Pathway and Likely Functions Both in the Nervous System and in Muscle Genetics, December 1, 2003; 165(4): 1805 - 1822. [Abstract] [Full Text] [PDF]

				I. A. Bany, M.-Q. Dong, and M. R. Koelle Genetic and Cellular Basis for Acetylcholine Inhibition of Caenorhabditis elegans Egg-Laying Behavior J. Neurosci., September 3, 2003; 23(22): 8060 - 8069. [Abstract] [Full Text] [PDF]

				B. D. Ackley, S. H. Kang, J. R. Crew, C. Suh, Y. Jin, and J. M. Kramer The Basement Membrane Components Nidogen and Type XVIII Collagen Regulate Organization of Neuromuscular Junctions in Caenorhabditis elegans J. Neurosci., May 1, 2003; 23(9): 3577 - 3587. [Abstract] [Full Text] [PDF]

				S. M. Trailovic, A. P. Robertson, C. L. Clark, and R. J. Martin Levamisole receptor phosphorylation: effects of kinase antagonists on membrane potential responses in Ascaris suum suggest that CaM kinase and tyrosine kinase regulate sensitivity to levamisole J. Exp. Biol., December 15, 2002; 205(24): 3979 - 3988. [Abstract] [Full Text] [PDF]

				W. M. Nuttley, S. Harbinder, and D. van der Kooy Regulation of Distinct Attractive and Aversive Mechanisms Mediating Benzaldehyde Chemotaxis in Caenorhabditis elegans Learn. Mem., May 1, 2001; 8(3): 170 - 181. [Abstract] [Full Text] [PDF]

				J. Kim, D. S. Poole, L. E. Waggoner, A. Kempf, D. S. Ramirez, P. A. Treschow, and W. R. Schafer Genes Affecting the Activity of Nicotinic Receptors Involved in Caenorhabditis elegans Egg-Laying Behavior Genetics, April 1, 2001; 157(4): 1599 - 1610. [Abstract] [Full Text]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help