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The Journal of Neuroscience, December 15, 2000, 20(24):9053-9058

The Light Response of ON Bipolar Neurons Requires G_o

Anuradha Dhingra¹, Arkady Lyubarsky², Meisheng Jiang³, Edward N. Pugh Jr², Lutz Birnbaumer³, Peter Sterling¹, and Noga Vardi¹

Departments of ¹ Neuroscience and ² Ophthalmology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, and ³ Department of Anesthesiology, University of California at Los Angeles, Los Angeles, California 90024

ON bipolar neurons in retina detect the glutamate released by rods and cones via metabotropic glutamate receptor 6 (mGluR6), whose cascade is unknown. The trimeric G-protein G_o might mediate this cascade because it colocalizes with mGluR6. To test this, we studied the retina in mice negative for the  subunit of G_o (G_o/). Retinal layering, key cell types, synaptic structure, and mGluR6 expression were all normal, as was the a-wave of the electroretinogram, which represents the rod and cone photocurrents. However, the b-wave of the electroretinogram, both rod- and cone-driven components, was entirely missing. Because the b-wave represents the massed response of ON bipolar cells, its loss in the G_o null mouse establishes that the light response of the ON bipolar cell requires G_o. This represents the first function to be defined in vivo for the  subunit of the most abundant G-protein of the brain .

Key words: G-protein; mGluR6; metabotropic glutamate receptor; retina; bipolar cells; electroretinogram; rod bipolar cells; second messenger cascade

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Copyright © 2000 Society for Neuroscience  0270-6474/00/20249053-06$05.00/0

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