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The Journal of Neuroscience, December 15, 2000, 20(24):9135-9144
Specialized Synapse-Associated Structures within the Calyx of
Held
Kevin C.
Rowland1, 3,
Nancy K.
Irby2, 3, and
George A.
Spirou1, 2, 3
Departments of 1 Physiology and
2 Otolaryngology, and 3 Sensory Neuroscience
Research Center, West Virginia University School of Medicine,
Morgantown, West Virginia 26506-9200
The calyx of Held exhibits fast glutamatergic neurotransmission at
high rates with low temporal jitter and has adapted specialized synaptic mechanisms to support its functional demands. We report the
presence in calyces of an atypical arrangement of subcellular organelles, called the mitochondria-associated adherens complex (MAC).
We demonstrate that MACs are located adjacent to synapses and contain
membranous elements linked with coated and uncoated vesicles.
Mitochondria that form MACs have more complex geometries than other
mitochondria within the calyx and can extend between clusters of
synapses. We estimate that the calyx contains 1600 MACs, 2400 synapses,
and 6200 readily releasable vesicles. We also identify synaptic vesicle
endocytotic regions close to MACs and synapses and hypothesize that
calyces are composed of multiple activity modules, each containing
machinery for vesicle release and recycling.
Key words:
auditory brainstem; calyx of Held; mitochondria; punctum
adherens; synapse; synaptic vesicle recycling
Copyright © 2000 Society for Neuroscience 0270-6474/00/20249135-10$05.00/0
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