 |
||||
|
||||
|
||||
|
||||
|
||||
Previous Article | Next Article
The Journal of Neuroscience, February 1, 2000, 20(3):958-968
Altered Entrainment and Feedback Loop Function Effected by a Mutant Period Protein
Peter Schotland, Melissa Hunter-Ensor, Todd Lawrence, and Amita Sehgal Howard Hughes Medical Institute, Department of Neuroscience, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104
The period (per) and timeless (tim) genes encode interacting components of the circadian clock. Levels and phosphorylation states of both proteins cycle with a circadian rhythm, and the proteins drive cyclic expression of their RNAs through a feedback mechanism that is, at least in part, negative. We report here that a hypophosphorylated mutant PER protein, produced by creating a small internal deletion, displays increased stability and low-amplitude oscillations, consistent with previous reports that phosphorylation is required for protein turnover. In addition, this protein appears to be defective in feedback repression because it is associated with relatively high levels of RNA and high levels of TIM. Transgenic flies carrying the mutant PER protein display a temperature-dependent shortening of circadian period and are impaired in their response to light, particularly to pulses of light in the late night that normally advance the phase of the rhythm. Interestingly, per RNA is induced by light in these flies, most likely because of the removal of the light-sensitive TIM protein, thus implicating a more direct role for TIM in transcriptional inhibition. These data have relevance for mechanisms of feedback repression, and they also address existing models for the differential behavioral response to light at different times of the night.
Key words: circadian rhythms; Drosophila; per; tim; entrainment to light; feedback
Copyright © 2000 Society for Neuroscience 0270-6474/00/203958-11$05.00/0
This article has been cited by other articles:
W.-F. Chen, J. Majercak, and I. Edery
Clock-Gated Photic Stimulation of Timeless Expression at Cold Temperatures and Seasonal Adaptation in Drosophila
J Biol Rhythms, August 1, 2006; 21(4): 256 - 271.
[Abstract] [PDF]
F. Preuss, J.-Y. Fan, M. Kalive, S. Bao, E. Schuenemann, E. S. Bjes, and J. L. Price
Drosophila doubletime Mutations Which either Shorten or Lengthen the Period of Circadian Rhythms Decrease the Protein Kinase Activity of Casein Kinase I
Mol. Cell. Biol., January 15, 2004; 24(2): 886 - 898.
[Abstract] [Full Text] [PDF]
L. J. Ashmore, S. Sathyanarayanan, D. W. Silvestre, M. M. Emerson, P. Schotland, and A. Sehgal
Novel Insights into the Regulation of the Timeless Protein
J. Neurosci., August 27, 2003; 23(21): 7810 - 7819.
[Abstract] [Full Text] [PDF]
C. M. Constance, C. B. Green, H. Tei, and G. D. Block
Bulla gouldiana period exhibits unique regulation at the mrnA and Protein Levels
J Biol Rhythms, October 1, 2002; 17(5): 413 - 427.
[Abstract] [PDF]
O. T. Shafer, M. Rosbash, and J. W. Truman
Sequential Nuclear Accumulation of the Clock Proteins Period and Timeless in the Pacemaker Neurons of Drosophila melanogaster
J. Neurosci., July 15, 2002; 22(14): 5946 - 5954.
[Abstract] [Full Text] [PDF]
G. Bloch, D. P. Toma, and G. E. Robinson
Behavioral Rhythmicity, Age, Division of Labor and period Expression in the Honey Bee Brain
J Biol Rhythms, October 1, 2001; 16(5): 444 - 456.
[Abstract] [PDF]
S. Bao, J. Rihel, E. Bjes, J.-Y. Fan, and J. L. Price
The Drosophila double-timeS Mutation Delays the Nuclear Accumulation of period Protein and Affects the Feedback Regulation of period mRNA
J. Neurosci., September 15, 2001; 21(18): 7117 - 7126.
[Abstract] [Full Text] [PDF]
M. Ivanchenko, R. Stanewsky, and J. M. Giebultowicz
Circadian Photoreception in Drosophila: Functions of Cryptochrome in Peripheral and Central Clocks
J Biol Rhythms, June 1, 2001; 16(3): 205 - 215.
[Abstract] [PDF]
|
|
|
|
 |
|