QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (39) Citing Articles via Google Scholar Google Scholar Articles by Xiong, Z.-Q. Articles by Stringer, J. L. Search for Related Content PubMed PubMed Citation Articles by Xiong, Z.-Q. Articles by Stringer, J. L.

 Previous Article  |  Next Article 

The Journal of Neuroscience, February 15, 2000, 20(4):1290-1296

Activity-Dependent Intracellular Acidification Correlates with the Duration of Seizure Activity

Zhi-Qi Xiong¹, Peter Saggau², and Janet L. Stringer^{1, 2}

¹ Department of Pharmacology and ² Division of Neuroscience, Baylor College of Medicine, Houston, Texas 77030

Synchronized neuronal activity (seizures) can appear in the presence or absence of synaptic transmission. Mechanisms of seizure initiation in each of these conditions have been studied, but relatively few studies have addressed seizure termination. In particular, how are seizures terminated in the absence of synaptic activity where there is no loss of excitatory drive or augmentation of inhibitory inputs? We have studied dynamic activity-dependent changes of intracellular pH in the absence of synaptic transmission using the fluorescent pH indicator carboxylseminaphthorhodafluo-1. During epileptiform activity we observed intracellular acidification, whereas between seizures the intracellular pH recovered. Experimental conditions that shortened the epileptiform discharge correlated with more rapid intracellular acidification. On the other hand, experimental manipulation of intracellular pH altered the duration of the seizure discharge, with acidification resulting in early termination of the epileptiform activity. These data show a direct relationship between the level of intracellular acidification and the duration of the seizures, suggesting that an intracellular pH-dependent process can terminate nonsynaptic neuronal synchronization.

Key words: intracellular pH; seizure termination; dentate gyrus; SNARF-1; hippocampus; neuronal synchronization

---

Copyright © 2000 Society for Neuroscience  0270-6474/00/2041290-07$05.00/0

This article has been cited by other articles:

				S. Jacobs, E. Ruusuvuori, S. T. Sipila, A. Haapanen, H. H. Damkier, I. Kurth, M. Hentschke, M. Schweizer, Y. Rudhard, L. M. Laatikainen, et al. Mice with targeted Slc4a10 gene disruption have small brain ventricles and show reduced neuronal excitability PNAS, January 8, 2008; 105(1): 311 - 316. [Abstract] [Full Text] [PDF]

				B. Lasztoczi and J. Kardos Cyclothiazide Prolongs Low [Mg2+]-Induced Seizure-Like Events J Neurophysiol, December 1, 2006; 96(6): 3538 - 3544. [Abstract] [Full Text] [PDF]

				M. Hentschke, M. Wiemann, S. Hentschke, I. Kurth, I. Hermans-Borgmeyer, T. Seidenbecher, T. J. Jentsch, A. Gal, and C. A. Hubner Mice with a Targeted Disruption of the Cl-/HCO3- Exchanger AE3 Display a Reduced Seizure Threshold Mol. Cell. Biol., January 1, 2006; 26(1): 182 - 191. [Abstract] [Full Text] [PDF]

				D. J. Pinto, S. L. Patrick, W. C. Huang, and B. W. Connors Initiation, Propagation, and Termination of Epileptiform Activity in Rodent Neocortex In Vitro Involve Distinct Mechanisms J. Neurosci., September 7, 2005; 25(36): 8131 - 8140. [Abstract] [Full Text] [PDF]

				M. CHESLER Regulation and Modulation of pH in the Brain Physiol Rev, October 1, 2003; 83(4): 1183 - 1221. [Abstract] [Full Text] [PDF]

				J. Mao, J. Wu, F. Chen, X. Wang, and C. Jiang Inhibition of G-protein-coupled Inward Rectifying K+ Channels by Intracellular Acidosis J. Biol. Chem., February 21, 2003; 278(9): 7091 - 7098. [Abstract] [Full Text] [PDF]

				D. Yu, G. S. Baird, R. Y. Tsien, and R. L. Davis Detection of Calcium Transients in Drosophila Mushroom Body Neurons with Camgaroo Reporters J. Neurosci., January 1, 2003; 23(1): 64 - 72. [Abstract] [Full Text] [PDF]

				S. S. Jahromi, K. Wentlandt, S. Piran, and P. L. Carlen Anticonvulsant Actions of Gap Junctional Blockers in an In Vitro Seizure Model J Neurophysiol, October 1, 2002; 88(4): 1893 - 1902. [Abstract] [Full Text] [PDF]

				J. Tabak, J. Rinzel, and M. J. O'Donovan The Role of Activity-Dependent Network Depression in the Expression and Self-Regulation of Spontaneous Activity in the Developing Spinal Cord J. Neurosci., November 15, 2001; 21(22): 8966 - 8978. [Abstract] [Full Text] [PDF]

				K. D. Micheva, R. W. Holz, and S. J. Smith Regulation of presynaptic phosphatidylinositol 4,5-biphosphate by neuronal activity J. Cell Biol., July 23, 2001; 154(2): 355 - 368. [Abstract] [Full Text] [PDF]

				M. R. Pelletier, P. A. Pahapill, P. S. Pennefather, and P. L. Carlen Analysis of Single KATP Channels in Mammalian Dentate Gyrus Granule Cells J Neurophysiol, November 1, 2000; 84(5): 2291 - 2301. [Abstract] [Full Text] [PDF]

				J. S. Schweitzer, H. Wang, Z.-Q. Xiong, and J. L. Stringer pH Sensitivity of Non-Synaptic Field Bursts in the Dentate Gyrus J Neurophysiol, August 1, 2000; 84(2): 927 - 933. [Abstract] [Full Text] [PDF]

				Z.-Q. Xiong and J. L. Stringer Extracellular pH Responses in CA1 and the Dentate Gyrus During Electrical Stimulation, Seizure Discharges, and Spreading Depression J Neurophysiol, June 1, 2000; 83(6): 3519 - 3524. [Abstract] [Full Text] [PDF]

				I. I Grichtchenko, I. Choi, X. Zhong, P. Bray-Ward, J. M. Russell, and W. F. Boron Cloning, Characterization, and Chromosomal Mapping of a Human Electroneutral Na+-driven Cl-HCO3 Exchanger J. Biol. Chem., March 9, 2001; 276(11): 8358 - 8363. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help