 |
||||
|
||||
|
||||
|
||||
Previous Article | Next Article
The Journal of Neuroscience, February 15, 2000, 20(4):1424-1434
Molecular Cloning and Functional Expression of the Potassium-Dependent Sodium-Calcium Exchanger from Human and Chicken Retinal Cone Photoreceptors
Clemens F. M. Prinsen, Robert T. Szerencsei, and Paul P. M. Schnetkamp Department of Physiology and Biophysics and the Medical Research Council Group on Ion Channels and Transporters, University of Calgary, Faculty of Medicine, Calgary, Alberta, Canada T2N 4N1
Light causes a rapid lowering of cytosolic free calcium in the outer segments of both retinal rod and cone photoreceptors. This light-induced lowering of calcium is caused by extrusion via a Na-Ca exchanger located in the rod and cone outer segment plasma membrane and plays a key role in the process of light adaptation. The Na-Ca exchanger in retinal rod outer segment was shown earlier to be a novel Na-Ca+K exchanger (NCKX), and its cDNA was obtained by molecular cloning from several mammalian species. On the other hand, the proper identity of the retinal cone Na-Ca exchanger, in terms of both functional characteristics (e.g., requirement for and transport of potassium) and molecular identity, has not yet been elucidated. Here, we report the molecular cloning, intraretinal localization by in situ hybridization, and initial functional characterization of the chicken and human cone-specific Na-Ca exchangers. In addition we report the chicken rod-specific NCKX. We identified NCKX transcripts in both human and chicken cones and observed strong potassium-dependent Na-Ca exchange activity after heterologous expression of human and chicken cone NCKX cDNAs in cultured insect cells. In situ hybridization in chicken retina showed abundant rod NCKX transcripts only in rod photoreceptors, whereas abundant cone NCKX transcripts were found in most, if not all, cone photoreceptors and also in a subpopulation of retinal ganglion cells. A detailed comparison with the previously described retinal rod and brain NCKX cDNAs is presented.
Key words: sodium-calcium exchange; retina; photoreceptor cells; ganglion cells; chicken; human; calcium homeostasis; in situ hybridization
Copyright © 2000 Society for Neuroscience 0270-6474/00/2041424-11$05.00/0
This article has been cited by other articles:
D.-G. Luo, T. Xue, and K.-W. Yau
How vision begins: An odyssey
PNAS, July 22, 2008; 105(29): 9855 - 9862.
[Abstract] [Full Text] [PDF]
R. S. Ginger, S. E. Askew, R. M. Ogborne, S. Wilson, D. Ferdinando, T. Dadd, A. M. Smith, S. Kazi, R. T. Szerencsei, R. J. Winkfein, et al.
SLC24A5 Encodes a trans-Golgi Network Protein with Potassium-dependent Sodium-Calcium Exchange Activity That Regulates Human Epidermal Melanogenesis
J. Biol. Chem., February 29, 2008; 283(9): 5486 - 5495.
[Abstract] [Full Text] [PDF]
O. Cuomo, R. Gala, G. Pignataro, F. Boscia, A. Secondo, A. Scorziello, A. Pannaccione, D. Viggiano, A. Adornetto, P. Molinaro, et al.
A Critical Role for the Potassium-Dependent Sodium-Calcium Exchanger NCKX2 in Protection against Focal Ischemic Brain Damage
J. Neurosci., February 27, 2008; 28(9): 2053 - 2063.
[Abstract] [Full Text] [PDF]
J. C. Corbo, C. A. Myers, K. A. Lawrence, A. P. Jadhav, and C. L. Cepko
A typology of photoreceptor gene expression patterns in the mouse
PNAS, July 17, 2007; 104(29): 12069 - 12074.
[Abstract] [Full Text] [PDF]
F. Visser and J. Lytton
K+-Dependent Na+/Ca2+ Exchangers: Key Contributors to Ca2+ Signaling
Physiology, June 1, 2007; 22(3): 185 - 192.
[Abstract] [Full Text] [PDF]
F. Visser, V. Valsecchi, L. Annunziato, and J. Lytton
Analysis of Ion Interactions with the K+ -dependent Na+/Ca+ Exchangers NCKX2, NCKX3, and NCKX4: IDENTIFICATION OF THR-551 AS A KEY RESIDUE IN DEFINING THE APPARENT K+ AFFINITY OF NCKX2
J. Biol. Chem., February 16, 2007; 282(7): 4453 - 4462.
[Abstract] [Full Text] [PDF]
H. F. Altimimi and P. P. M. Schnetkamp
Na+-dependent Inactivation of the Retinal Cone/Brain Na+/Ca2+-K+ Exchanger NCKX2
J. Biol. Chem., February 9, 2007; 282(6): 3720 - 3729.
[Abstract] [Full Text] [PDF]
C. Paillart, R. J. Winkfein, P. P.M. Schnetkamp, and J. I. Korenbrot
Functional Characterization and Molecular Cloning of the K+-dependent Na+/Ca2+ Exchanger in Intact Retinal Cone Photoreceptors
J. Gen. Physiol., January 1, 2007; 129(1): 1 - 16.
[Abstract] [Full Text] [PDF]
X.-F. Li, L. Kiedrowski, F. Tremblay, F. R. Fernandez, M. Perizzolo, R. J. Winkfein, R. W. Turner, J. S. Bains, D. E. Rancourt, and J. Lytton
Importance of K+-dependent Na+/Ca2+-exchanger 2, NCKX2, in Motor Learning and Memory
J. Biol. Chem., March 10, 2006; 281(10): 6273 - 6282.
[Abstract] [Full Text] [PDF]
R. Rizzuto and T. Pozzan
Microdomains of Intracellular Ca2+: Molecular Determinants and Functional Consequences
Physiol Rev, January 1, 2006; 86(1): 369 - 408.
[Abstract] [Full Text] [PDF]
K.-J. Kang, T. G. Kinjo, R. T. Szerencsei, and P. P. M. Schnetkamp
Residues Contributing to the Ca2+ and K+ Binding Pocket of the NCKX2 Na+/Ca2+-K+ Exchanger
J. Biol. Chem., February 25, 2005; 280(8): 6823 - 6833.
[Abstract] [Full Text] [PDF]
K.-J. Kang, Y. Shibukawa, R. T. Szerencsei, and P. P. M. Schnetkamp
Substitution of a Single Residue, Asp575, Renders the NCKX2 K+-dependent Na+/Ca2+ Exchanger Independent of K+
J. Biol. Chem., February 25, 2005; 280(8): 6834 - 6839.
[Abstract] [Full Text] [PDF]
X. Cai and J. Lytton
The Cation/Ca2+ Exchanger Superfamily: Phylogenetic Analysis and Structural Implications
Mol. Biol. Evol., September 1, 2004; 21(9): 1692 - 1703.
[Abstract] [Full Text] [PDF]
X. Cai and J. Lytton
Molecular Cloning of a Sixth Member of the K+-dependent Na+/Ca2+ Exchanger Gene Family, NCKX6
J. Biol. Chem., February 13, 2004; 279(7): 5867 - 5876.
[Abstract] [Full Text] [PDF]
M.-H. Kim, S.-h. Lee, K. H. Park, W.-K. Ho, and S.-H. Lee
Distribution of K+-Dependent Na+/Ca2+ Exchangers in the Rat Supraoptic Magnocellular Neuron Is Polarized to Axon Terminals
J. Neurosci., December 17, 2003; 23(37): 11673 - 11680.
[Abstract] [Full Text] [PDF]
X.-F. Li, A. S. Kraev, and J. Lytton
Molecular Cloning of a Fourth Member of the Potassium-dependent Sodium-Calcium Exchanger Gene Family, NCKX4
J. Biol. Chem., December 6, 2002; 277(50): 48410 - 48417.
[Abstract] [Full Text] [PDF]
X. Cai, K. Zhang, and J. Lytton
A Novel Topology and Redox Regulation of the Rat Brain K+-dependent Na+/Ca2+ Exchanger, NCKX2
J. Biol. Chem., December 6, 2002; 277(50): 48923 - 48930.
[Abstract] [Full Text] [PDF]
S.-H. Lee, M.-H. Kim, K. H. Park, Y. E. Earm, and W.-K. Ho
K+-Dependent Na+/Ca2+ Exchange Is a Major Ca2+ Clearance Mechanism in Axon Terminals of Rat Neurohypophysis
J. Neurosci., August 15, 2002; 22(16): 6891 - 6899.
[Abstract] [Full Text] [PDF]
D. Sharon, H. Yamamoto, T. L. McGee, V. Rabe, R. T. Szerencsei, R. J. Winkfein, C. F. M. Prinsen, C. S. Barnes, S. Andreasson, G. A. Fishman, et al.
Mutated Alleles of the Rod and Cone Na-Ca+K-Exchanger Genes in Patients with Retinal Diseases
Invest. Ophthalmol. Vis. Sci., June 1, 2002; 43(6): 1971 - 1979.
[Abstract] [Full Text] [PDF]
Y.-H. Su and V. D. Vacquier
A flagellar K+-dependent Na+/Ca2+ exchanger keeps Ca2+ low in sea urchin spermatozoa
PNAS, May 14, 2002; 99(10): 6743 - 6748.
[Abstract] [Full Text] [PDF]
A. Kraev, B. D. Quednau, S. Leach, X.-F. Li, H. Dong, R. Winkfein, M. Perizzolo, X. Cai, R. Yang, K. D. Philipson, et al.
Molecular Cloning of a Third Member of the Potassium-dependent Sodium-Calcium Exchanger Gene Family, NCKX3
J. Biol. Chem., June 15, 2001; 276(25): 23161 - 23172.
[Abstract] [Full Text] [PDF]
H. Dong, P. E. Light, R. J. French, and J. Lytton
Electrophysiological Characterization and Ionic Stoichiometry of the Rat Brain K+-dependent Na+/Ca2+ Exchanger, NCKX2
J. Biol. Chem., July 6, 2001; 276(28): 25919 - 25928.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|