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The Journal of Neuroscience, April 15, 2000, 20(8):2766-2773

NMDA-Dependent Modulation of Hippocampal Kainate Receptors by Calcineurin and Ca²⁺/Calmodulin-Dependent Protein Kinase

Andrea Ghetti and Stephen F. Heinemann

Molecular Neurobiology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037

Neurotransmitter receptor function can be influenced by the phosphorylation state of the receptor or of associated proteins. Here we show that kainate receptors expressed in cultured hippocampal neurons can be modulated by Ca²⁺/calmodulin-dependent phosphatase (calcineurin) and Ca²⁺/calmodulin-dependent kinase (CaMK). Ca²⁺ influx through NMDA receptor or voltage-sensitive calcium channels resulted in a transient depression of the kainate receptor current. This calcium-induced depression of the kainate receptor current depended on the activation of the phosphatase calcineurin. The amplitude of the kainate receptor currents returned to the baseline level in ~9 sec ( = 3.6 sec), and the recovery of the current amplitude depended on CaMK activity. The effect on kainate receptor currents was dependent on the frequency of NMDA receptor activation. Although low-frequency (0.1 Hz) NMDA application induced depression followed by recovery of the kainate receptor currents, higher frequency (1 Hz) NMDA applications induced a more prolonged depression. Kainate receptors have been shown to modulate synaptic transmission by both presynaptic and postsynaptic mechanisms. Our results suggest that synaptic activity mediated by NMDA receptors, or other routes of Ca²⁺ influx, may, in turn, modulate the function of kainate receptors.

Key words: hippocampal neuron; kainate receptor; NMDA receptor; calcineurin; CaMK; receptor modulation; calcium imaging

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Copyright © 2000 Society for Neuroscience  0270-6474/00/2082766-08$05.00/0

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