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Published Online
on May 10, 2002
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The Journal of Neuroscience, 2002, 22:RC223:1-5
RAPID COMMUNICATION
Receptors with Different Affinities Mediate Phasic and Tonic GABAA Conductances in Hippocampal NeuronsBrandon M. Stell and Istvan Mody Interdepartmental Program in Molecular, Cellular, and Integrative Physiology and Departments of Neurology and Physiology, University of California Los Angeles School of Medicine, Los Angeles, California 90095
A tonic inhibitory conductance mediated by GABAA receptors that supplements the phasic inhibition produced by IPSCs has been identified in the hippocampus and cerebellum. It is presently unknown whether tonic and phasic inhibitions are mediated by GABAA receptors with different subunit assemblies. Here we demonstrate that a low concentration (200 nM) of the highly specific competitive GABAA antagonist SR95531 (gabazine) reduces phasic inhibition in hippocampal granule cells by 71% but has no effect on tonic inhibition, whereas a high concentration (10 µM) of the antagonist blocked both conductances. These findings are consistent with tonic and phasic conductances being mediated by different GABAA receptor subtypes with different affinities for GABA.
Key words: GABA affinity; tonic inhibition; phasic inhibition; competitive GABAA antagonist; SR95531; IPSC amplitude quantification
Copyright © Society for Neuroscience 0270-6474//$05.00/0
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