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The Journal of Neuroscience, January 1, 2001, 21(1):150-158

Independent Anchoring and Assembly Mechanisms of INAD Signaling Complexes in Drosophila Photoreceptors

Susan Tsunoda¹, Yumei Sun¹, Emiko Suzuki², and Charles Zuker¹

¹ Howard Hughes Medical Institute and Departments of Biology and Neurosciences, University of California at San Diego, La Jolla, California 92093-0649, and ² The Institute of Medical Science, The University of Tokyo and Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, Tokyo, Japan

In Drosophila photoreceptors the multivalent PDZ protein INAD organizes the phototransduction cascade into a macromolecular signaling complex containing the effector PLC, the light-activated TRP channels, and a regulatory PKC. Previously, we showed that the subcellular localization of INAD signaling complexes is critical for signaling. Now we have examined how INAD complexes are anchored and assembled in photoreceptor cells. We find that trp mutants, or transgenic flies expressing inaD alleles that disrupt the interaction between INAD and TRP, cause the mislocalization of the entire transduction complex. The INAD-TRP interaction is not required for targeting but rather for anchoring of complexes, because INAD and TRP can be targeted independently of each other. We also show that, in addition to its scaffold role, INAD functions to preassemble transduction complexes. Preassembly of signaling complexes helps to ensure that transduction complexes with the appropriate composition end up in the proper location. This may be a general mechanism used by cells to target different signaling machinery to the pertinent subcellular location.

Key words: INAD; signaling complex; transducisome; Drosophila; phototransduction; subcellular localization; signal transduction; anchoring; assembly

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Copyright © 2001 Society for Neuroscience  0270-6474/01/211150-09$05.00/0

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