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The Journal of Neuroscience, January 1, 2001, 21(1):330-339

Heterogeneity of Hippocampal GABA_A Receptors: Regulation by Corticosterone

Miles Orchinik¹, Steven S. Carroll¹, Yi-Huey Li¹, Bruce S. McEwen², and Nancy G. Weiland²

¹ Department of Biology, Arizona State University, Tempe, Arizona 85287-1501, and ² Laboratory of Neuroendocrinology Rockefeller University, New York, New York 10021

Chronic stressors produce changes in hippocampal neurochemistry, neuronal morphology, and hippocampal-dependent learning and memory processes. In rats, stress-induced changes in CA3 apical dendritic structure are mediated by corticosterone (CORT) acting, in part, on excitatory amino acid neurotransmission. CORT also alters GABA-mediated inhibitory neurotransmission, so the GABA_A receptor system may also contribute to dendritic remodeling and other stress-related changes in hippocampal function. A previous study indicated that chronic CORT treatment produces complex changes in GABA_A receptor subunit mRNA levels, so we hypothesized that CORT alters the pharmacological properties of hippocampal GABA_A receptors. To test this, adult male rats were treated with CORT or vehicle pellets for 10 d, after which we quantified [³⁵S]t-butylbicyclophosphorothionate ([³⁵S]TBPS) and [³H]flunitrazepam binding to GABA_A receptors using in vitro receptor autoradiography. Pharmacological properties of receptors were assessed by examining the allosteric regulation of binding at both sites by GABA and 5-pregnane-3,21-diol-20-one (THDOC), an endogenous anxiolytic steroid. We found striking regional differences in the modulation of [³⁵S]TBPS binding, particularly between strata radiatum and strata oriens, suggesting a functional heterogeneity among hippocampal GABA_A receptors even within the apical versus basal dendrites of pyramidal neurons. Furthermore, we found that CORT treatment decreased the negative modulation of hippocampal [³⁵S]TBPS binding by both GABA and THDOC and increased the enhancement of [³H]flunitrazepam binding by GABA and THDOC in the dentate gyrus. Together, these data suggest that prolonged exposure to stress levels of corticosteroids may alter hippocampal inhibitory tone by regulating the pharmacological properties of GABA_A receptors in discrete dendritic subfields.

Key words: chronic stress; corticosterone; hippocampus; GABA_A receptors; neurosteroid; 5-pregnane-3,21-diol-20-one; testosterone

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Copyright © 2001 Society for Neuroscience  0270-6474/01/211330-10$05.00/0

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