QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (28) Citing Articles via Google Scholar Google Scholar Articles by Lee, C. J. Articles by MacDermott, A. B. Search for Related Content PubMed PubMed Citation Articles by Lee, C. J. Articles by MacDermott, A. B. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB CALCIUM COMPOUNDS CALCIUM, ELEMENTAL

 Previous Article  |  Next Article 

The Journal of Neuroscience, July 1, 2001, 21(13):4572-4581

Kainate Receptors Expressed by a Subpopulation of Developing Nociceptors Rapidly Switch from High to Low Ca²⁺ Permeability

C. Justin Lee¹, Haeyoung Kong⁴, M. Chiara Manzini^{2, 3}, Cristóvão Albuquerque¹, Moses V. Chao⁴, and Amy B. MacDermott^{1, 3}

¹ Department of Physiology and Cellular Biophysics, ² Department of Pathology, and the ³ Center for Neurobiology and Behavior, Columbia University, New York, New York 10032, and ⁴ Molecular Neurobiology Program, Skirball Institute, New York University Medical Center, New York, New York 10016

Dorsal root ganglion (DRG) neurons first express kainate receptor subunits, predominantly GluR5, during embryonic development. In the DRG and throughout the nervous system, substantial editing of GluR5 mRNA occurs with developmental maturation (Bernard et al., 1999). The accompanying change in Ca²⁺ permeability of functional kainate receptors that is the predicted outcome of this developmental regulation of mRNA editing has not been investigated. Here we report that kainate receptors on DRG neurons from late embryonic and newborn rats are predominantly Ca²⁺ permeable but then become fully Ca²⁺ impermeable later in the first postnatal week. Using multiple markers for nociceptor subpopulations, we show that this switch in Ca²⁺ permeability is not caused by the appearance of a new subpopulation of nociceptors with different receptor properties. Instead, the change in Ca²⁺ permeability matches the time course of post-transcriptional RNA editing of GluR5 at the Q/R site within the pore of the channel, indicating that the change is probably caused by developmentally regulated RNA editing. We also report that, on the basis of the strong correlation of receptor expression with expression of the surface markers LA4, isolectin B4, and LD2, kainate receptors are present on C-fiber-type neurons projecting to lamina II of spinal cord dorsal horn. These results raise the possibility that kainate receptors in their Ca²⁺-permeable form serve a developmental role in synapse formation between this population of C-fibers and their targets in the spinal cord dorsal horn. Thereafter, the receptors may serve a new function that does not require Ca²⁺ permeability.

Key words: dorsal root ganglion; nociceptor; kainate receptor; calcium permeability; capsaicin; LD2; LA4; IB4; lamina II; C-fiber

---

Copyright © 2001 Society for Neuroscience  0270-6474/01/21134572-10$05.00/0

This article has been cited by other articles:

				S. Kesavapany, V. Patel, Y.-L. Zheng, T. K. Pareek, M. Bjelogrlic, W. Albers, N. Amin, H. Jaffe, J. S. Gutkind, M. J. Strong, et al. Inhibition of Pin1 Reduces Glutamate-induced Perikaryal Accumulation of Phosphorylated Neurofilament-H in Neurons Mol. Biol. Cell, September 1, 2007; 18(9): 3645 - 3655. [Abstract] [Full Text] [PDF]

				S. E. Lauri, M. Segerstrale, A. Vesikansa, F. Maingret, C. Mulle, G. L. Collingridge, J. T. R. Isaac, and T. Taira Endogenous Activation of Kainate Receptors Regulates Glutamate Release and Network Activity in the Developing Hippocampus J. Neurosci., May 4, 2005; 25(18): 4473 - 4484. [Abstract] [Full Text] [PDF]

				S. Xia, J. Yang, Y. Su, J. Qian, E. Ma, and G. G. Haddad Identification of new targets of Drosophila pre-mRNA adenosine deaminase Physiol Genomics, January 20, 2005; 20(2): 195 - 202. [Abstract] [Full Text] [PDF]

				D.-h. Youn and M. Randic Modulation of excitatory synaptic transmission in the spinal substantia gelatinosa of mice deficient in the kainate receptor GluR5 and/or GluR6 subunit J. Physiol., March 15, 2004; 555(3): 683 - 698. [Abstract] [Full Text] [PDF]

				F. A. Ene, P. H. M. Kullmann, D. C. Gillespie, and K. Kandler Glutamatergic Calcium Responses in the Developing Lateral Superior Olive: Receptor Types and Their Specific Activation by Synaptic Activity Patterns J Neurophysiol, October 1, 2003; 90(4): 2581 - 2591. [Abstract] [Full Text] [PDF]

				C. Schmauss and J. R. Howe RNA Editing of Neurotransmitter Receptors in the Mammalian Brain Sci. Signal., May 21, 2002; 2002(133): pe26 - pe26. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help