 |
||||
|
||||
|
||||
|
||||
|
||||
Previous Article | Next Article
The Journal of Neuroscience, July 1, 2001, 21(13):4609-4624
Differential Expression of Genes Encoding Subthreshold-Operating Voltage-Gated K+ Channels in Brain
M. J. Saganich, E. Machado, and B. Rudy Department of Physiology and Neuroscience and Department of Biochemistry, New York University School of Medicine, New York, New York 10016
The members of the three subfamilies (eag, erg, and elk) of the ether-a-go-go (EAG) family of potassium channel pore-forming subunits express currents that, like the M-current (IM), could have considerable influence on the subthreshold properties of neuronal membranes, and hence the control of excitability. A nonradioactive in situ hybridization (NR-ISH) study of the distribution of the transcripts encoding the eight known EAG family subunits in rat brain was performed to identify neuronal populations in which the physiological roles of EAG channels could be studied. These distributions were compared with those of the mRNAs encoding the components of the classical M-current (Kcnq2 and Kcnq3). NR-ISH was combined with immunohistochemistry to specific neuronal markers to help identify expressing neurons. The results show that each EAG subunit has a specific pattern of expression in rat brain. EAG and Kcnq transcripts are prominent in several types of excitatory neurons in the cortex and hippocampus; however, only one of these channel components (erg1) was consistently expressed in inhibitory interneurons in these areas. Some neuronal populations express more than one product of the same subfamily, suggesting that the subunits may form heteromeric channels in these neurons. Many neurons expressed multiple EAG family and Kcnq transcripts, such as CA1 pyramidal neurons, which contained Kcnq2, Kcnq3, eag1, erg1, erg3, elk2, and elk3. This indicates that the subthreshold current in many neurons may be complex, containing different components mediated by a number of channels with distinct properties and neuromodulatory responses.
Key words: EAG; ERG; ELK; Kcnq; potassium channels; M-currents; mRNA; nonradioactive in situ hybridization; immunohistochemistry; GABAergic interneurons; parvalbumin; brain
Copyright © 2001 Society for Neuroscience 0270-6474/01/21134609-16$05.00/0
This article has been cited by other articles:
M. Pessia, I. Servettini, R. Panichi, L. Guasti, S. Grassi, A. Arcangeli, E. Wanke, and V. E. Pettorossi
ERG voltage-gated K+ channels regulate excitability and discharge dynamics of the medial vestibular nucleus neurones
J. Physiol., October 15, 2008; 586(20): 4877 - 4890.
[Abstract] [Full Text] [PDF]
H. Vacher, D. P. Mohapatra, and J. S. Trimmer
Localization and Targeting of Voltage-Dependent Ion Channels in Mammalian Central Neurons
Physiol Rev, October 1, 2008; 88(4): 1407 - 1447.
[Abstract] [Full Text] [PDF]
C. C. Canavier, S. A. Oprisan, J. C. Callaway, H. Ji, and P. D. Shepard
Computational Model Predicts a Role for ERG Current in Repolarizing Plateau Potentials in Dopamine Neurons: Implications for Modulation of Neuronal Activity
J Neurophysiol, November 1, 2007; 98(5): 3006 - 3022.
[Abstract] [Full Text] [PDF]
P. D. Shepard, C. C. Canavier, and E. S. Levitan
Ether-a-go-go Related Gene Potassium Channels: What's All the Buzz About?
Schizophr Bull, November 1, 2007; 33(6): 1263 - 1269.
[Abstract] [Full Text] [PDF]
M. R. Kasten, B. Rudy, and M. P. Anderson
Differential regulation of action potential firing in adult murine thalamocortical neurons by Kv3.2, Kv1, and SK potassium and N-type calcium channels
J. Physiol., October 15, 2007; 584(2): 565 - 582.
[Abstract] [Full Text] [PDF]
R. Balu and B. W. Strowbridge
Opposing Inward and Outward Conductances Regulate Rebound Discharges in Olfactory Mitral Cells
J Neurophysiol, March 1, 2007; 97(3): 1959 - 1968.
[Abstract] [Full Text] [PDF]
K. M. Hurley, S. Gaboyard, M. Zhong, S. D. Price, J. R. A. Wooltorton, A. Lysakowski, and R. A. Eatock
M-Like K+ Currents in Type I Hair Cells and Calyx Afferent Endings of the Developing Rat Utricle
J. Neurosci., October 4, 2006; 26(40): 10253 - 10269.
[Abstract] [Full Text] [PDF]
L. Forti, E. Cesana, J. Mapelli, and E. D'Angelo
Ionic mechanisms of autorhythmic firing in rat cerebellar Golgi cells
J. Physiol., August 1, 2006; 574(3): 711 - 729.
[Abstract] [Full Text] [PDF]
C. Weber, F. M. de Queiroz, B. R. Downie, A. Suckow, W. Stuhmer, and L. A. Pardo
Silencing the Activity and Proliferative Properties of the Human EagI Potassium Channel by RNA Interference
J. Biol. Chem., May 12, 2006; 281(19): 13030 - 13037.
[Abstract] [Full Text] [PDF]
R. Restano-Cassulini, Y. V. Korolkova, S. Diochot, G. Gurrola, L. Guasti, L. D. Possani, M. Lazdunski, E. V. Grishin, A. Arcangeli, and E. Wanke
Species Diversity and Peptide Toxins Blocking Selectivity of Ether-a-go-go-Related Gene Subfamily K+ Channels in the Central Nervous System
Mol. Pharmacol., May 1, 2006; 69(5): 1673 - 1683.
[Abstract] [Full Text] [PDF]
Z. Pan, T. Kao, Z. Horvath, J. Lemos, J.-Y. Sul, S. D. Cranstoun, V. Bennett, S. S. Scherer, and E. C. Cooper
A common ankyrin-G-based mechanism retains KCNQ and NaV channels at electrically active domains of the axon.
J. Neurosci., March 8, 2006; 26(10): 2599 - 2613.
[Abstract] [Full Text] [PDF]
A. P. Hegle, D. D. Marble, and G. F. Wilson
A voltage-driven switch for ion-independent signaling by ether-a-go-go K+ channels
PNAS, February 21, 2006; 103(8): 2886 - 2891.
[Abstract] [Full Text] [PDF]
N. M. Kirchberger, I. Wulfsen, J. R. Schwarz, and C. K. Bauer
Effects of TRH on heteromeric rat erg1a/1b K+ channels are dominated by the rerg1b subunit
J. Physiol., February 15, 2006; 571(1): 27 - 42.
[Abstract] [Full Text] [PDF]
J. P. A. Bannister, B. Chanda, F. Bezanilla, and D. M. Papazian
Optical detection of rate-determining ion-modulated conformational changes of the ether-a-go-go K+ channel voltage sensor
PNAS, December 20, 2005; 102(51): 18718 - 18723.
[Abstract] [Full Text] [PDF]
W. Shen, S. E. Hamilton, N. M. Nathanson, and D. J. Surmeier
Cholinergic Suppression of KCNQ Channel Currents Enhances Excitability of Striatal Medium Spiny Neurons
J. Neurosci., August 10, 2005; 25(32): 7449 - 7458.
[Abstract] [Full Text] [PDF]
M. P. G. Korsgaard, B. P. Hartz, W. D. Brown, P. K. Ahring, D. Strobaek, and N. R. Mirza
Anxiolytic Effects of Maxipost (BMS-204352) and Retigabine via Activation of Neuronal Kv7 Channels
J. Pharmacol. Exp. Ther., July 1, 2005; 314(1): 282 - 292.
[Abstract] [Full Text] [PDF]
E. Zagha, A. Ozaita, S. Y. Chang, M. S. Nadal, U. Lin, M. J. Saganich, T. McCormack, K. O. Akinsanya, S. Y. Qi, and B. Rudy
DPP10 Modulates Kv4-mediated A-type Potassium Channels
J. Biol. Chem., May 13, 2005; 280(19): 18853 - 18861.
[Abstract] [Full Text] [PDF]
P. Sturm, S. Wimmers, J. R Schwarz, and C. K Bauer
Extracellular potassium effects are conserved within the rat erg K+ channel family
J. Physiol., April 15, 2005; 564(2): 329 - 345.
[Abstract] [Full Text] [PDF]
W. Hirdes, M. Schweizer, K. S Schuricht, S. S Guddat, I. Wulfsen, C. K Bauer, and J. R Schwarz
Fast erg K+ currents in rat embryonic serotonergic neurones
J. Physiol., April 1, 2005; 564(1): 33 - 49.
[Abstract] [Full Text] [PDF]
R. E. Garcia-Ferreiro, D. Kerschensteiner, F. Major, F. Monje, W. Stuhmer, and L. A. Pardo
Mechanism of Block of hEag1 K+ Channels by Imipramine and Astemizole
J. Gen. Physiol., September 27, 2004; 124(4): 301 - 317.
[Abstract] [Full Text] [PDF]
W. Hirdes, L. F. Horowitz, and B. Hille
Muscarinic modulation of erg potassium current
J. Physiol., August 15, 2004; 559(1): 67 - 84.
[Abstract] [Full Text] [PDF]
S. Schreiber, I. Erchova, U. Heinemann, and A. V. M. Herz
Subthreshold Resonance Explains the Frequency-Dependent Integration of Periodic as Well as Random Stimuli in the Entorhinal Cortex
J Neurophysiol, July 1, 2004; 92(1): 408 - 415.
[Abstract] [Full Text] [PDF]
A. Zou, Z. Lin, M. Humble, C. D. Creech, P. K. Wagoner, D. Krafte, T. J. Jegla, and A. D. Wickenden
Distribution and functional properties of human KCNH8 (Elk1) potassium channels
Am J Physiol Cell Physiol, December 1, 2003; 285(6): C1356 - C1366.
[Abstract] [Full Text] [PDF]
T. Sacco, A. Bruno, E. Wanke, and F. Tempia
Functional Roles of an ERG Current Isolated in Cerebellar Purkinje Neurons
J Neurophysiol, September 1, 2003; 90(3): 1817 - 1828.
[Abstract] [Full Text] [PDF]
M M Shah, M Mistry, S J Marsh, D A Brown, and P Delmas
Molecular correlates of the M-current in cultured rat hippocampal neurons
J. Physiol., October 1, 2002; 544(1): 29 - 37.
[Abstract] [Full Text] [PDF]
A. Pannaccione, P. Castaldo, E. Ficker, L. Annunziato, and M. Taglialatela
Histidines 578 and 587 in the S5-S6 Linker of the Human Ether-a-gogo Related Gene-1 K+ Channels Confer Sensitivity to Reactive Oxygen Species
J. Biol. Chem., March 8, 2002; 277(11): 8912 - 8919.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|