QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (35) Citing Articles via Google Scholar Google Scholar Articles by Jang, I.-S. Articles by Akaike, N. Search for Related Content PubMed PubMed Citation Articles by Jang, I.-S. Articles by Akaike, N.

 Previous Article  |  Next Article 

The Journal of Neuroscience, August 15, 2001, 21(16):5962-5972

Contribution of the Na-K-Cl Cotransporter on GABA_A Receptor-Mediated Presynaptic Depolarization in Excitatory Nerve Terminals

Il-Sung Jang, Hyo-Jin Jeong, and Norio Akaike

Cellular and System Physiology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan

GABA_A receptor-mediated responses manifest as either hyperpolarization or depolarization according to the intracellular Cl concentration ([Cl]_i). Here, we report a novel functional interaction between the Na-K-Cl cotransporter (NKCC) and GABA_A receptor actions on glutamatergic presynaptic nerve terminals projecting to ventromedial hypothalamic (VMH) neurons. The activation of presynaptic GABA_A receptors depolarizes the presynaptic nerve terminals and facilitates spontaneous glutamate release by activating TTX-sensitive Na⁺ channels and high-threshold Ca²⁺ channels. This depolarizing action of GABA was caused by an outwardly directed Cl driving force for GABA_A receptors; that is, the [Cl]_i of glutamatergic nerve terminals was higher than that predicted for a passive distribution. The higher [Cl]_i was generated by bumetanide-sensitive NKCCs and was responsible for the GABA-induced presynaptic depolarization. Thus, GABA_A receptor-mediated modulation of spontaneous glutamatergic transmission may contribute to the development and regulation of VMH function as well as to the excitability of VMH neurons themselves.

Key words: sEPSCs; presynaptic GABA_A receptors; intraterminal Cl concentration; NKCC; mechanical dissociation; VMH; GABA-induced depolarization

---

Copyright © 2001 Society for Neuroscience  0270-6474/01/21165962-11$05.00/0

This article has been cited by other articles:

				U. Kim and L.-y. Chung Dual GABAergic Synaptic Response of Fast Excitation and Slow Inhibition in the Medial Habenula of Rat Epithalamus J Neurophysiol, September 1, 2007; 98(3): 1323 - 1332. [Abstract] [Full Text] [PDF]

				H. Alle and J. R. P. Geiger GABAergic Spill-Over Transmission onto Hippocampal Mossy Fiber Boutons J. Neurosci., January 24, 2007; 27(4): 942 - 950. [Abstract] [Full Text] [PDF]

				G. D. Price and L. O. Trussell Estimate of the chloride concentration in a central glutamatergic terminal: a gramicidin perforated-patch study on the calyx of held. J. Neurosci., November 1, 2006; 26(44): 11432 - 11436. [Abstract] [Full Text] [PDF]

				B. B. Pond, K. Berglund, T. Kuner, G. Feng, G. J. Augustine, and R. D. Schwartz-Bloom The Chloride Transporter Na+-K+-Cl- Cotransporter Isoform-1 Contributes to Intracellular Chloride Increases after In Vitro Ischemia J. Neurosci., February 1, 2006; 26(5): 1396 - 1406. [Abstract] [Full Text] [PDF]

				J. Beck, B. Lenart, D. B. Kintner, and D. Sun Na-K-Cl Cotransporter Contributes to Glutamate-Mediated Excitotoxicity J. Neurosci., June 15, 2003; 23(12): 5061 - 5068. [Abstract] [Full Text] [PDF]

				V. Balakrishnan, M. Becker, S. Lohrke, H. G. Nothwang, E. Guresir, and E. Friauf Expression and Function of Chloride Transporters during Development of Inhibitory Neurotransmission in the Auditory Brainstem J. Neurosci., May 15, 2003; 23(10): 4134 - 4145. [Abstract] [Full Text] [PDF]

				S. Uchida, E. Noda, Y. Kakazu, Y. Mizoguchi, N. Akaike, and J. Nabekura Allopregnanolone enhancement of GABAergic transmission in rat medial preoptic area neurons Am J Physiol Endocrinol Metab, December 1, 2002; 283(6): E1257 - E1265. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help