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The Journal of Neuroscience, August 15, 2001, 21(16):6000-6007

A High-Efficiency Protein Transduction System Demonstrating the Role of PKA in Long-Lasting Long-Term Potentiation

Masayuki Matsushita1, Kazuhito Tomizawa1, Akiyoshi Moriwaki1, Sheng-Tian Li1, Hiroaki Terada1, 2, and Hideki Matsui1

1 First Department of Physiology and 2 Department of Neurological Surgery, Okayama University Medical School, Okayama 700-8558, Japan

Proteins and peptides have been demonstrated to penetrate across the plasma membrane of eukaryotic cells by protein transduction domains. We show that protein transduction by 11 arginine (11R) is an efficient method of delivering proteins into the neurons of brain slices. Here, we demonstrate that PKA inhibitory peptide, fused with 11R and nuclear localization signal, delivers the peptide exclusively into the nuclear compartment of neurons in brain slices. This inhibitory peptide blocked both cAMP responsive element-binding protein phosphorylation and long-lasting long-term potentiation (LTP) induction, but not early LTP. These results highlight transduction of proteins and peptides into specific neuronal subcellular compartments in brain slices as a powerful tool for studying neuronal plasticity.

Key words: protein transduction; poly arginine; PKA; CREB; LTP; brain slice

Copyright © 2001 Society for Neuroscience  0270-6474/01/21166000-08$05.00/0


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