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The Journal of Neuroscience, August 15, 2001, 21(16):6105-6114
Spine Formation and Correlated Assembly of Presynaptic and Postsynaptic Molecules
Shigeo Okabe1, 2, 4, Akiko Miwa3, 4, and Haruo Okado3, 4 1 Department of Anatomy and Cell Biology, School of Medicine, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, 113-8519, Japan, 2 Laboratory of Molecular Neurobiology, National Institute of Bioscience and Human Technology, Tsukuba, Ibaraki 305-8566, Japan, 3 Department of Neurobiology, Tokyo Metropolitan Institute for Neuroscience, Fuchu, Tokyo 183-8526, Japan, and 4 Core Research for Evolution Science and Technology, Japan Science and Technology Corporation, Kawaguchi 332-0012, Japan
Hippocampal pyramidal neurons in culture showed a developmental shift in synapse distribution from dendritic shafts to spines. Using dual wavelength time-lapse fluorescence microscopy, we analyzed the morphogenesis of three synaptic components: dendritic spines, postsynaptic densities (PSDs), and presynaptic vesicles. Local assembly of a major PSD protein, PSD-95, was spatially and temporally correlated with spine morphogenesis. Clustering of postsynaptic PSD-95 and that of a predominant synaptic vesicle protein, synaptophysin, were also correlated. In contrast, pre-existing PSD-95 clusters in dendritic shafts were preferentially eliminated without promoting spine formation. The local and stepwise assembly of synaptic components at the contact sites between dendritic protrusions and axons explains the developmental remodeling of excitatory synapses.
Key words: synaptogenesis; dendritic spines; postsynaptic density; green fluorescent protein; fluorescence microscopy; hippocampus
Copyright © 2001 Society for Neuroscience 0270-6474/01/21166105-10$05.00/0
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