QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (21) Citing Articles via Google Scholar Google Scholar Articles by Staugaitis, S. M. Articles by Goldman, J. E. Search for Related Content PubMed PubMed Citation Articles by Staugaitis, S. M. Articles by Goldman, J. E. Pubmed/NCBI databases Compound via MeSH Substance via MeSH

 Previous Article  |  Next Article 

The Journal of Neuroscience, August 15, 2001, 21(16):6195-6205

Aldolase C/Zebrin II Expression in the Neonatal Rat Forebrain Reveals Cellular Heterogeneity within the Subventricular Zone and Early Astrocyte Differentiation

Susan M. Staugaitis¹, Marielba Zerlin², Richard Hawkes³, Joel M. Levine⁴, and James E. Goldman²

¹ Department of Neurosciences (NC30), The Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, ² Department of Pathology, Columbia University, College of Physicians and Surgeons, New York, New York 10032, ³ Department of Cell Biology and Anatomy, Faculty of Medicine, University of Calgary, Alberta, Canada, T2N 4N1, and ⁴ Department of Neurobiology and Behavior, State University of New York, Stony Brook, New York 11794

During late gestational and early postnatal development, proliferating cells in the subventricular zones of the lateral ventricles (SVZ) migrate into the gray and white matter of the forebrain and differentiate into astrocytes and oligodendrocytes. Because the cellular composition and structure of the neonatal SVZ is poorly understood, we performed a differential display PCR screen to identify genes preferentially expressed therein. One highly expressed gene encoded aldolase C. We used a specific monoclonal antibody, aldolase C/zebrin II (ALDC/ZII), in combination with markers of glial lineage and proliferation, to characterize the cells that express this gene. In the neonatal SVZ, ALDC/ZII-positive cells, which are generally polygonal and display several processes, have a nonuniform spatial distribution. They do not express vimentin, GFAP, or NG2. A subset of ALDC/ZII-positive cells incorporates bromodeoxyuridine, but progenitors identified by -galactosidase expression after infection with recombinant BAG virus do not show ALDC/ZII immunoreactivity. Outside of the SVZ, -galactosidase-positive/ALDC/ZII-positive cells have an astrocytic phenotype, suggesting that immunoreactivity was acquired after exit from the SVZ. These studies demonstrate that the neonatal SVZ is composed of different populations of cells that can be characterized by their antigenic phenotype, their proliferative capacity, and their spatial distributions. Nonrandom distributions of different cell types within the SVZ may permit the formation of microenvironments that stimulate the production of cells with specific potentials at appropriate points in development. Analysis of ALDC/ZII expression by astrocyte lineage cells in the neonatal cerebral cortex and white matter may reveal insights into the phenotype and behavior of undifferentiated astrocyte progenitors.

Key words: subventricular zone; astrocyte; oligodendrocyte; progenitor cells; cell lineage; aldolase C; zebrin II; NG2 chondroitin sulfate proteoglycan; vimentin

---

Copyright © 2001 Society for Neuroscience  0270-6474/01/21166195-11$05.00/0

This article has been cited by other articles:

				R. E. Ventura and J. E. Goldman Dorsal Radial Glia Generate Olfactory Bulb Interneurons in the Postnatal Murine Brain J. Neurosci., April 18, 2007; 27(16): 4297 - 4302. [Abstract] [Full Text] [PDF]

				C. A. G. Marshall, B. G. Novitch, and J. E. Goldman Olig2 Directs Astrocyte and Oligodendrocyte Formation in Postnatal Subventricular Zone Cells J. Neurosci., August 10, 2005; 25(32): 7289 - 7298. [Abstract] [Full Text] [PDF]

				R. Canete-Soler, K. S. Reddy, D. R. Tolan, and J. Zhai Aldolases A and C Are Ribonucleolytic Components of a Neuronal Complex That Regulates the Stability of the Light-Neurofilament mRNA J. Neurosci., April 27, 2005; 25(17): 4353 - 4364. [Abstract] [Full Text] [PDF]

				C. A. G. Marshall and J. E. Goldman Subpallial Dlx2-Expressing Cells Give Rise to Astrocytes and Oligodendrocytes in the Cerebral Cortex and White Matter J. Neurosci., November 15, 2002; 22(22): 9821 - 9830. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help