 |
 Previous Article | Next Article 
The Journal of Neuroscience, August 15, 2001, 21(16):6387-6394
Group I Metabotropic Glutamate Receptors Elicit Epileptiform
Discharges in the Hippocampus through PLC 1 Signaling
Shih-Chieh
Chuang1,
Riccardo
Bianchi1,
Daesoo
Kim2,
Hee-Sup
Shin2, and
Robert K. S.
Wong1
1 Department of Physiology and Pharmacology, State
University of New York-Health Science Center at Brooklyn, Brooklyn,
New York 11203, and 2 National CRI Center for Calcium and
Learning, Department of Life Science, Pohang University of Science and
Technology, Pohang, 790-784, Korea
Activation of metabotropic glutamate receptors (mGluRs) produces
multiple effects in cortical neurons, resulting in the emergence of
network activities including epileptiform discharges. The cellular mechanisms underlying such network responses are largely unknown. We
examined the properties of group I mGluR-mediated cellular responses in
CA3 neurons and attempted to determine their role in the generation of
the network activities. Group I mGluR stimulation causes depolarization
of hippocampal neurons. This depolarization is primarily mediated by
two sets of conductance change: the opening of a voltage-dependent
cationic conductance (mediating ImGluR(V)) and the closing of a voltage-independent (background)
K+ conductance. ImGluR(V)
was no longer elicited by group I mGluR agonists in the presence of
U73122, a phospholipase C (PLC) blocker. Also, the current could not be
activated in hippocampal CA3 neurons from PLC 1 knock-out mice. In
contrast, suppression of PLC signaling did not affect the group I
mGluR-mediated suppression of background K+
conductance. Thus, the suppression of the background
K+ conductance occurred upstream to PLC activation,
whereas the generation of ImGluR(V) occurred
downstream to PLC activation. Group I mGluR agonists normally elicited
rhythmic single cell and population burst responses in the CA3 neurons.
In the absence of an ImGluR(V) response, CA3
neurons in slices prepared from PLC1 / mutant mice could no
longer generate these responses. The results suggest that
ImGluR(V) expression in CA3 hippocampal neuron is PLC1-dependent and that
ImGluR(V) plays a necessary role in the
generation of rhythmic single cell bursts and synchronized epileptiform
discharges in the CA3 region of the hippocampus.
Key words:
PLC1; signal transduction; epileptiform discharges; mGluRs; hippocampus; CA3 population oscillations
Copyright © 2001 Society for Neuroscience 0270-6474/01/21166387-08$05.00/0
This article has been cited by other articles:
|
|
|
|
 
J. Zhong, S.-C. Chuang, R. Bianchi, W. Zhao, H. Lee, A. A. Fenton, R. K. S. Wong, and H. Tiedge
BC1 Regulation of Metabotropic Glutamate Receptor-Mediated Neuronal Excitability
J. Neurosci.,
August 12, 2009;
29(32):
9977 - 9986.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Bianchi, S.-C. Chuang, W. Zhao, S. R. Young, and R. K. S. Wong
Cellular Plasticity for Group I mGluR-Mediated Epileptogenesis
J. Neurosci.,
March 18, 2009;
29(11):
3497 - 3507.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Ferraguti, L. Crepaldi, and F. Nicoletti
Metabotropic Glutamate 1 Receptor: Current Concepts and Perspectives
Pharmacol. Rev.,
December 1, 2008;
60(4):
536 - 581.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. R. Young, R. Bianchi, and R. K. S. Wong
Signaling Mechanisms Underlying Group I mGluR-Induced Persistent AHP Suppression in CA3 Hippocampal Neurons
J Neurophysiol,
March 1, 2008;
99(3):
1105 - 1118.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Schwendt and J. F. McGinty
Regulator of G-Protein Signaling 4 Interacts with Metabotropic Glutamate Receptor Subtype 5 in Rat Striatum: Relevance to Amphetamine Behavioral Sensitization
J. Pharmacol. Exp. Ther.,
November 1, 2007;
323(2):
650 - 657.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-W. Sohn, D. Lee, H. Cho, W. Lim, H.-S. Shin, S.-H. Lee, and W.-K. Ho
Receptor-specific inhibition of GABAB-activated K+ currents by muscarinic and metabotropic glutamate receptors in immature rat hippocampus
J. Physiol.,
April 15, 2007;
580(2):
411 - 422.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. O. McNamara, Y. Z. Huang, and A. S. Leonard
Molecular Signaling Mechanisms Underlying Epileptogenesis
Sci. Signal.,
October 10, 2006;
2006(356):
re12 - re12.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Fellin, M. Gomez-Gonzalo, S. Gobbo, G. Carmignoto, and P. G. Haydon
Astrocytic Glutamate Is Not Necessary for the Generation of Epileptiform Neuronal Activity in Hippocampal Slices
J. Neurosci.,
September 6, 2006;
26(36):
9312 - 9322.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Bianchi, S.-C. Chuang, and R. K. S. Wong
Pharmacology of a Slowly Inactivating Outward Current in Hippocampal CA3 Pyramidal Neurons
J Neurophysiol,
September 1, 2006;
96(3):
1116 - 1123.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Wettschureck, M. van der Stelt, H. Tsubokawa, H. Krestel, A. Moers, S. Petrosino, G. Schutz, V. Di Marzo, and S. Offermanns
Forebrain-Specific Inactivation of Gq/G11 Family G Proteins Results in Age-Dependent Epilepsy and Impaired Endocannabinoid Formation
Mol. Cell. Biol.,
August 1, 2006;
26(15):
5888 - 5894.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Shin, D. Kim, R. Bianchi, R. K. S. Wong, and H.-S. Shin
Genetic dissection of theta rhythm heterogeneity in mice
PNAS,
December 13, 2005;
102(50):
18165 - 18170.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. C. Cuellar, E. L. Griffith, and L. R. Merlin
Contrasting Roles of Protein Kinase C in Induction Versus Suppression of Group I mGluR-Mediated Epileptogenesis In Vitro
J Neurophysiol,
November 1, 2005;
94(5):
3643 - 3647.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. R. Young, S.-C. Chuang, and R. K. S. Wong
Modulation of afterpotentials and firing pattern in guinea pig CA3 neurones by group I metabotropic glutamate receptors
J. Physiol.,
January 15, 2004;
554(2):
371 - 385.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Zhao, R. Bianchi, M. Wang, and R. K. S. Wong
Extracellular Signal-Regulated Kinase 1/2 Is Required for the Induction of Group I Metabotropic Glutamate Receptor-Mediated Epileptiform Discharges
J. Neurosci.,
January 7, 2004;
24(1):
76 - 84.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Stoop, F. Conquet, B. Zuber, L. L. Voronin, and E. Pralong
Activation of Metabotropic Glutamate 5 and NMDA Receptors Underlies the Induction of Persistent Bursting and Associated Long-Lasting Changes in CA3 Recurrent Connections
J. Neurosci.,
July 2, 2003;
23(13):
5634 - 5644.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Burgo, G. Carmignoto, P. Pizzo, T. Pozzan, and C. Fasolato
Paradoxical Ca2+ rises induced by low external Ca2+ in rat hippocampal neurones
J. Physiol.,
June 1, 2003;
549(2):
537 - 552.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W.-M. Zho, J.-L. You, C.-C. Huang, and K.-S. Hsu
The Group I Metabotropic Glutamate Receptor Agonist (S)-3,5-Dihydroxyphenylglycine Induces a Novel Form of Depotentiation in the CA1 Region of the Hippocampus
J. Neurosci.,
October 15, 2002;
22(20):
8838 - 8849.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. C. Lee, R. K. S. Wong, S.-C. Chuang, H.-S. Shin, and R. Bianchi
Role of Synaptic Metabotropic Glutamate Receptors in Epileptiform Discharges in Hippocampal Slices
J Neurophysiol,
October 1, 2002;
88(4):
1625 - 1633.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-C. Chuang, W. Zhao, S. R Young, F. Conquet, R. Bianchi, and R. K S Wong
Activation of group I mGluRs elicits different responses in murine CA1 and CA3 pyramidal cells
J. Physiol.,
May 15, 2002;
541(1):
113 - 121.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
