QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) A correction has been published Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (70) Citing Articles via Google Scholar Google Scholar Articles by Persico, A. M. Articles by Keller, F. Search for Related Content PubMed PubMed Citation Articles by Persico, A. M. Articles by Keller, F. Pubmed/NCBI databases Gene GEO Profiles HomoloGene UniGene Compound via MeSH Substance via MeSH

 Previous Article  |  Next Article 

The Journal of Neuroscience, September 1, 2001, 21(17):6862-6873

Barrel Pattern Formation Requires Serotonin Uptake by Thalamocortical Afferents, and Not Vesicular Monoamine Release

Antonio M. Persico¹, Elisa Mengual², Rainald Moessner³, Scott F. Hall⁴, Randal S. Revay⁴, Ichiro Sora⁴, Jon Arellano⁵, Javier DeFelipe⁵, José Manuel Giménez-Amaya², Monica Conciatori¹, Ramona Marino¹, Alfonso Baldi¹, Simona Cabib⁶, Tiziana Pascucci⁶, George R. Uhl⁴, Dennis L. Murphy⁷, K. Peter Lesch³, and Flavio Keller¹

¹ Laboratory of Neuroscience, Università "Campus Bio-Medico," 00155 Rome, Italy, ² Departamento de Anatomia, Facultad de Medicina, Universidad de Navarra, 31008 Pamplona, Spain, ³ Department of Psychiatry, University of Wuerzburg, 97080 Wuerzburg, Germany, ⁴ Molecular Neurobiology Branch, Intramural Research Program/National Institute on Drug Abuse/National Institutes of Health, Baltimore, Maryland 21224, ⁵ Instituto Cajal, Consejo Superior de Investigación Cientifica, 28029 Madrid, Spain, ⁶ Department of Psychology, Università "La Sapienza," 00185 Rome, Italy, and ⁷ Laboratory of Clinical Science, National Institute of Mental Health/National Institutes of Health, Bethesda, Maryland 20892

Thalamocortical neurons innervating the barrel cortex in neonatal rodents transiently store serotonin (5-HT) in synaptic vesicles by expressing the plasma membrane serotonin transporter (5-HTT) and the vesicular monoamine transporter (VMAT2). 5-HTT knock-out (ko) mice reveal a nearly complete absence of 5-HT in the cerebral cortex by immunohistochemistry, and of barrels, both at P7 and adulthood. Quantitative electron microscopy reveals that 5-HTT ko affects neither the density of synapses nor the length of synaptic contacts in layer IV. VMAT2 ko mice, completely lacking activity-dependent vesicular release of monoamines including 5-HT, also show a complete lack of 5-HT in the cortex but display largely normal barrel fields, despite sometimes markedly reduced postnatal growth. Transient 5-HTT expression is thus required for barrel pattern formation, whereas activity-dependent vesicular 5-HT release is not.

Key words: barrel; homologous recombination; knock-out; monoamine; p-chlorophenylalanine; serotonin; serotonin transporter; vesicular monoamine transporter; GABA transporter; whisker

---

Copyright © 2001 Society for Neuroscience  0270-6474/01/21176862-12$05.00/0

This article has been cited by other articles:

				M. Minlebaev, Y. Ben-Ari, and R. Khazipov NMDA Receptors Pattern Early Activity in the Developing Barrel Cortex In Vivo Cereb Cortex, March 1, 2009; 19(3): 688 - 696. [Abstract] [Full Text] [PDF]

				K. A. Young, W. L. Bonkale, L. A. Holcomb, P. B. Hicks, and D. C. German Major depression, 5HTTLPR genotype, suicide and antidepressant influences on thalamic volume The British Journal of Psychiatry, April 1, 2008; 192(4): 285 - 289. [Abstract] [Full Text] [PDF]

				M. F. Casanova and C. R. Tillquist Encephalization, Emergent Properties, and Psychiatry: A Minicolumnar Perspective Neuroscientist, February 1, 2008; 14(1): 101 - 118. [Abstract] [PDF]

				A. Takeuchi, T. Hamasaki, E. D. Litwack, and D. D.M. O'Leary Novel IgCAM, MDGA1, Expressed in Unique Cortical Area- and Layer-Specific Patterns and Transiently by Distinct Forebrain Populations of Cajal-Retzius Neurons Cereb Cortex, July 1, 2007; 17(7): 1531 - 1541. [Abstract] [Full Text] [PDF]

				C Altamura, M. Dell'Acqua, R Moessner, D. Murphy, K. Lesch, and A. M. Persico Altered Neocortical Cell Density and Layer Thickness in Serotonin Transporter Knockout Mice: A Quantitation Study Cereb Cortex, June 1, 2007; 17(6): 1394 - 1401. [Abstract] [Full Text] [PDF]

				M. Inan and M. C. Crair Development of Cortical Maps: Perspectives From the Barrel Cortex Neuroscientist, February 1, 2007; 13(1): 49 - 61. [Abstract] [PDF]

				H.-C. Lu, D. A. Butts, P. S. Kaeser, W.-C. She, R. Janz, and M. C. Crair Role of efficient neurotransmitter release in barrel map development. J. Neurosci., March 8, 2006; 26(10): 2692 - 2703. [Abstract] [Full Text] [PDF]

				H. C. Prasad, C.-B. Zhu, J. L. McCauley, D. J. Samuvel, S. Ramamoorthy, R. C. Shelton, W. A. Hewlett, J. S. Sutcliffe, and R. D. Blakely Human serotonin transporter variants display altered sensitivity to protein kinase G and p38 mitogen-activated protein kinase PNAS, August 9, 2005; 102(32): 11545 - 11550. [Abstract] [Full Text] [PDF]

				T. Esaki, M. Cook, K. Shimoji, D. L. Murphy, L. Sokoloff, and A. Holmes Developmental disruption of serotonin transporter function impairs cerebral responses to whisker stimulation in mice PNAS, April 12, 2005; 102(15): 5582 - 5587. [Abstract] [Full Text] [PDF]

				D. L. Murphy, A. Lerner, G. Rudnick, and K.-P. Lesch Serotonin Transporter: Gene, Genetic Disorders, and Pharmacogenetics Mol. Interv., April 1, 2004; 4(2): 109 - 123. [Abstract] [Full Text] [PDF]

				M.-T. Liu, S. Rayport, Y. Jiang, D. L. Murphy, and M. D. Gershon Expression and function of 5-HT3 receptors in the enteric neurons of mice lacking the serotonin transporter Am J Physiol Gastrointest Liver Physiol, December 1, 2002; 283(6): G1398 - G1411. [Abstract] [Full Text] [PDF]

				S. L. Donovan, L. A. Mamounas, A. M. Andrews, M. E. Blue, and J. S. McCasland GAP-43 Is Critical for Normal Development of the Serotonergic Innervation in Forebrain J. Neurosci., May 1, 2002; 22(9): 3543 - 3552. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help