 |
 Previous Article | Next Article 
The Journal of Neuroscience, January 15, 2001, 21(2):741-749
Intensity-Dependent, Rapid Activation of Presynaptic Metabotropic
Glutamate Receptors at a Central Synapse
Gautam B.
Awatramani and
Malcolm M.
Slaughter
Department of Physiology and Biophysics and Department of
Ophthalmology, State University of New York, Buffalo, New York
14214
Synaptic signals from retinal bipolar cells were monitored by
measuring EPSCs in ganglion cells voltage-clamped at  70 mV. Spontaneous EPSCs were strongly suppressed by
L-2-amino-4-phosphonobutyrate (AP-4), an agonist at
group III metabotropic glutamate receptors (mGluRs). Agonists of group
I or II mGluRs were ineffective. AP-4 also suppressed ganglion cell
EPSCs evoked by bipolar cell stimulation using potassium puffs, sucrose
puffs, or zaps of current (0.5-1 µA). In addition, AP-4 suppressed
Off EPSCs evoked by dim-light stimuli. This indicates that group III
mGluRs mediate a direct suppression of bipolar cell transmitter
release. An mGluR antagonist, (RS)- -cyclopropyl-4-phosphonophenylyglycine (CPPG),
blocked the action of AP-4. When bipolar cells were weakly stimulated,
AP-4 produced a large suppression of the EPSC, but CPPG alone had
little effect. Conversely, when bipolar cells were strongly stimulated, CPPG produced an enhancement of the EPSC, but AP-4 alone had little effect. This indicates that endogenous feedback regulates bipolar cell
transmitter release and that the dynamic range of the presynaptic metabotropic autoreceptor is similar to that of the postsynaptic ionotropic receptor. Furthermore, the feedback is rapid and
intensity-dependent. Hence, concomitant activation of presynaptic and
postsynaptic glutamate receptors shapes the responses of ganglion cells.
Key words:
synaptic transmission; retina; bipolar cell; AP-4; CPPG; ganglion cell
Copyright © 2001 Society for Neuroscience 0270-6474/01/212741-09$05.00/0
This article has been cited by other articles:
|
|
|
|
 
M. Hou, L. Duan, and M. M. Slaughter
Synaptic inhibition by glycine acting at a metabotropic receptor in tiger salamander retina
J. Physiol.,
June 15, 2008;
586(12):
2913 - 2926.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. D. Field, A. Sher, J. L. Gauthier, M. Greschner, J. Shlens, A. M. Litke, and E. J. Chichilnisky
Spatial Properties and Functional Organization of Small Bistratified Ganglion Cells in Primate Retina
J. Neurosci.,
November 28, 2007;
27(48):
13261 - 13272.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. C. Renteria, N. Tian, J. Cang, S. Nakanishi, M. P. Stryker, and D. R. Copenhagen
Intrinsic ON Responses of the Retinal OFF Pathway Are Suppressed by the ON Pathway.
J. Neurosci.,
November 15, 2006;
26(46):
11857 - 11869.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. A. Rusakov
Ca2+-Dependent Mechanisms of Presynaptic Control at Central Synapses
Neuroscientist,
August 1, 2006;
12(4):
317 - 326.
[Abstract]
[PDF]
|
|
|
|
|
|
|
N. Hosoi, I. Arai, and M. Tachibana
Group III Metabotropic Glutamate Receptors and Exocytosed Protons Inhibit L-Type Calcium Currents in Cones But Not in Rods
J. Neurosci.,
April 20, 2005;
25(16):
4062 - 4072.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Y. Wong, A. R. Adolph, and J. E. Dowling
Retinal Bipolar Cell Input Mechanisms in Giant Danio. I. Electroretinographic Analysis
J Neurophysiol,
January 1, 2005;
93(1):
84 - 93.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Nawy
Desensitization of the mGluR6 transduction current in tiger salamander On bipolar cells
J. Physiol.,
July 1, 2004;
558(1):
137 - 146.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Acuna-Goycolea, Y. Li, and A. N. van den Pol
Group III Metabotropic Glutamate Receptors Maintain Tonic Inhibition of Excitatory Synaptic Input to Hypocretin/Orexin Neurons
J. Neurosci.,
March 24, 2004;
24(12):
3013 - 3022.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. J. Palmer, C. Hull, J. Vigh, and H. von Gersdorff
Synaptic Cleft Acidification and Modulation of Short-Term Depression by Exocytosed Protons in Retinal Bipolar Cells
J. Neurosci.,
December 10, 2003;
23(36):
11332 - 11341.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. A. Zaghloul, K. Boahen, and J. B. Demb
Different Circuits for ON and OFF Retinal Ganglion Cells Cause Different Contrast Sensitivities
J. Neurosci.,
April 1, 2003;
23(7):
2645 - 2654.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. K. Hoffpauir and E. L. Gleason
Activation of mGluR5 Modulates GABAA Receptor Function in Retinal Amacrine Cells
J Neurophysiol,
October 1, 2002;
88(4):
1766 - 1776.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Koulen and J. H. Brandstatter
Pre- and Postsynaptic Sites of Action of mGluR8a in the Mammalian Retina
Invest. Ophthalmol. Vis. Sci.,
June 1, 2002;
43(6):
1933 - 1940.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Mitra and M. M. Slaughter
Calcium-induced Transitions between the Spontaneous Miniature Outward and the Transient Outward Currents in Retinal Amacrine Cells
J. Gen. Physiol.,
April 2, 2002;
119(4):
373 - 388.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T Ohtake, H Yasuda, H Takahashi, T Goto, K Suzuki, K Yonemura, and A Hishida
Decreased plasma and cerebrospinal fluid glutamine concentrations in a patient with bialaphos poisoning
Human and Experimental Toxicology,
August 1, 2001;
20(8):
429 - 434.
[Abstract]
[PDF]
|
|
|
|
|
|
|
P. Mitra and M. M. Slaughter
Calcium-induced Transitions between the Spontaneous Miniature Outward and the Transient Outward Currents in Retinal Amacrine Cells
J. Gen. Physiol.,
April 2, 2002;
119(4):
373 - 388.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
