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The Journal of Neuroscience, October 15, 2001, 21(20):7871-7880
Region-Specific Developmental Specialization of GABA-Glycine
Cosynapses in Laminas I-II of the Rat Spinal Dorsal Horn
A. Florence
Keller1,
Jeffrey A. M.
Coull2, 3,
Nadège
Chéry2,
Pierrick
Poisbeau1, and
Yves
De Koninck2, 3
1 Laboratoire de Neurophysiologie Cellulaire et
Intégrée, Centre National de la Recherche Scientifique
Unité Mixte de Recherche 7519, Université Louis
Pasteur, 67084 Strasbourg cedex, France,
2 Department of Pharmacology and Therapeutics, McGill
University, Montréal, Québec, Canada H3G 1Y6, and
3 Neurobiologie Cellulaire, Centre de Recherche
Université Laval Robert-Giffard, Beauport, Québec, Canada
G1J 2G3
The spinal dorsal horn is the first level of the CNS in which
nociceptive input from sensory afferents is integrated and transmitted. Although inhibitory control in this region has a crucial impact on pain
transmission, the respective contribution of GABA and glycine to this
inhibition remains elusive. We have previously documented co-release of
GABA and glycine at the same inhibitory synapse in spinal laminas I-II
of adult rats [older than postnatal day 30 (P30)]. However,
despite this co-release, individual miniature inhibitory postsynaptic
currents (mIPSCs) were mediated by either glycine receptors (GlyR) or
GABAA receptors (GABAAR), yet never by the two
together. In contrast, recent studies of ventral horn immature
inhibitory synapses ( P21) reported individual mIPSCs that were
mediated by both GABAARs and GlyRs. This raises the question of whether mixed mIPSCs are present in immature lamina I-II
neurons yet are lost through a maturation-dependent synaptic specialization. To test this, we recorded mIPSCs using patch-clamp techniques in lamina I-II neurons in spinal slices taken at different stages of development. We found that, in neurons younger than P23, both
GlyR-only and GABAAR-only mIPSCs could be recorded, in
addition to mixed GABAAR-GlyR mIPSCs. With maturation
however, both lamina I-II neurons gradually discontinued
exhibiting mixed mIPSCs, although with differing patterns of
specialization. Yet, at all developmental stages, benzodiazepine
administration could unmask mixed mIPSCs. Together, these findings
indicate that, although GABA and glycine are continually co-released
throughout development, junctional codetection ceases by adulthood.
This indicates an age-dependent postsynaptic tuning of inhibitory
synapses that occurs in a region-specific manner.
Key words:
pain; nociception; development; plasticity; inhibition; cotransmission; mIPSCs; GABAA; quantal release; substantia gelatinosa; marginal layer; silent synapse
Copyright © 2001 Society for Neuroscience 0270-6474/01/21207871-10$05.00/0
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