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The Journal of Neuroscience, October 15, 2001, 21(20):8091-8107

RNA-Binding Protein Musashi2: Developmentally Regulated Expression in Neural Precursor Cells and Subpopulations of Neurons in Mammalian CNS

Shin-ichi Sakakibara^{1, 2}, Yuki Nakamura^{1, 3}, Hitoshi Satoh⁴, and Hideyuki Okano^{1, 2, 3}

¹ Division of Neuroanatomy, Department of Neuroscience, Biomedical Research Center, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan, ² Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan, ³ Department of Physiology, Keio University School of Medicine, Shinjuku-ku, Tokyo 160-8582, Japan, and ⁴ Division of Pathology, Department of Cancer Research, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo 108-8639, Japan

Musashi1 (Msi1) is a mammalian neural RNA-binding protein highly enriched in neural precursor cells that are capable of generating both neurons and glia during embryonic and postnatal CNS development. Here, we identified Musashi2 (Msi2), a novel mammalian RNA-binding protein that exhibits high sequence similarity to Msi1. The Msi2 transcript appeared to be distributed ubiquitously in a wide variety of tissues, consistent with the mRNA distribution of its Xenopus homolog, xrp1. However, the present study revealed cell type-specific and developmentally regulated expression of Msi2 in the mammalian CNS. Interestingly, Msi2 was expressed prominently in precursor cells in the ventricular zone and subventricular zone with the same pattern as Msi1 throughout CNS development. In the postnatal and adult CNS, this concurrent expression of Msi2 and Msi1 was seen in cells of the astrocyte lineage, including ependymal cells, a possible source for postnatal CNS stem cells. During neurogenesis, the expression of both Msi2 and Msi1 was lost in most postmitotic neurons, whereas Msi2 expression persisted in a subset of neuronal lineage cells, such as parvalbumin-containing GABA neurons in the neocortex and neurons in several nuclei of the basal ganglia. Msi2 may have a unique role that is required for the generation and/or maintenance of specific neuronal lineages. Furthermore, in vitro studies showed that Msi2 and Msi1 have similar RNA-binding specificity. These two RNA-binding proteins may exert common functions in neural precursor cells by regulating gene expression at the post-transcriptional level.

Key words: RNA-binding protein; Musashi2; Musashi1; CNS development; neural precursor cells; CNS stem cell; astrocyte; parvalbumin; GABAergic neuron

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Copyright © 2001 Society for Neuroscience  0270-6474/01/21208091-17$05.00/0

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