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The Journal of Neuroscience, October 15, 2001, 21(20):8247-8261

The Enterins: A Novel Family of Neuropeptides Isolated from the Enteric Nervous System and CNS of Aplysia

Y. Furukawa¹, K. Nakamaru¹, H. Wakayama¹, Y. Fujisawa³, H. Minakata³, S. Ohta², F. Morishita¹, O. Matsushima¹, L. Li⁴, E. Romanova⁴, J. V. Sweedler⁴, J. H. Park⁵, A. Romero⁵, E. C. Cropper⁵, N. C. Dembrow⁵, J. Jing⁵, K. R. Weiss⁵, and F. S. Vilim⁵

¹ Department of Biological Science, Faculty of Science, and ² Instrumental Center for Chemical Analysis, Hiroshima University, Higashi-Hiroshima 739-8526, Japan, ³ Suntory Institute for Bioorganic Research, Shimamoto, Mishima, Osaka 618-8503, Japan, ⁴ Department of Chemistry and Beckman Institute, University of Illinois, Urbana, Illinois 61801, and ⁵ Department of Physiology and Biophysics, Mount Sinai School of Medicine, New York, New York 10029

To identify neuropeptides that have a broad spectrum of actions on the feeding system of Aplysia, we searched for bioactive peptides that are present in both the gut and the CNS. We identified a family of structurally related nonapeptides and decapeptides (enterins) that are present in the gut and CNS of Aplysia, and most of which share the HSFVamide sequence at the C terminus. The structure of the enterin precursor deduced from cDNA cloning predicts 35 copies of 20 different enterins. Northern analysis, in situ hybridization, and immunocytochemistry show that the enterins are abundantly present in the CNS and the gut of Aplysia. Using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry we characterized the enterin-precursor processing, demonstrated that all of the precursor-predicted enterins are present, and determined post-translational modifications of various enterins. Enterin-positive neuronal somata and processes were found in the gut, and enterins inhibited contractions of the gut. In the CNS, the cerebral and buccal ganglia, which control feeding, contained the enterins. Enterin was also present in the nerve that connects these two ganglia. Enterins reduced the firing of interneurons B4/5 during feeding motor programs. Such enterin-induced reduction of firing also occurred when excitability of B4/5 was tested directly. Because reduction of B4/5 activity corresponds to a switch from egestive to ingestive behaviors, enterin may contribute to such program switching. Furthermore, because enterins are present throughout the nervous system, they may also play a regulatory role in nonfeeding behaviors of Aplysia.

Key words: enteric nervous system; neuropeptide; mollusc; Aplysia; cDNA cloning; immunohistochemistry; in situ hybridization; MALDI-TOF MS

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Copyright © 2001 Society for Neuroscience  0270-6474/01/21208247-15$05.00/0

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