QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (39) Citing Articles via Google Scholar Google Scholar Articles by Rodriguez, J. A. Articles by Dotti, C. G. Search for Related Content PubMed PubMed Citation Articles by Rodriguez, J. A. Articles by Dotti, C. G.

 Previous Article  |  Next Article 

The Journal of Neuroscience, November 1, 2001, 21(21):8387-8395

Plasma Membrane Ganglioside Sialidase Regulates Axonal Growth and Regeneration in Hippocampal Neurons in Culture

Jose Abad Rodriguez^{2, 3}, Eugenia Piddini³, Takafumi Hasegawa¹, Taeko Miyagi¹, and Carlos G. Dotti^{2, 3}

¹ Miyagi Prefectural Cancer Center, Division of Biochemistry, Medeshima-Shiode, Natori, Miyagi, 981-1293 Japan, ² Cavalieri Ottolenghi Scientific Institute, Universita degli Studi di Torino, A. O. San Luigi Gonzaga, Regione Gonzole 10, 10043 Orbassano (TO), Italy, and ³ Cell Biology and Biophysics Program, European Molecular Biology Laboratory, 69012 Heidelberg, Germany

It has been long recognized that the ganglioside GM1 plays a role in axonal growth and neuronal differentiation. However, the involvement of plasma membrane GM1 has been difficult to elucidate. This is possible now thanks to the recent cloning of plasma membrane ganglioside sialidase (PMGS), the enzyme responsible for the localized hydrolysis of oligosialogangliosides into GM1. In this work we show that PMGS mRNA and protein levels are high at early developmental stages of the hippocampus and low in adulthood both in vivo and in vitro. We also demonstrate that inhibition of PMGS activity blocks axonal elongation, whereas the increase in PMGS activity dramatically enhances axon growth and accelerates the polarization of cytoskeletal proteins. Finally, we show that axotomy close to the cell body in PMGS overexpressing neurons results in the regrowth of the original axon instead of randomly, as is the case in control neurons. In all, these results imply that PMGS activity through the modulation of GM1 surface levels is an important component of the machinery controlling axonal growth. We hypothesize that increasing PMGS activity in the adult nervous system may be useful to improve regeneration after nerve damage.

Key words: plasma membrane sialidase; ganglioside; hippocampal neuron; axonal growth; axonal regeneration

---

Copyright © 2001 Society for Neuroscience  0270-6474/01/21218387-09$05.00/0

This article has been cited by other articles:

				G. Wu, Z.-H. Lu, A. G. Obukhov, M. C. Nowycky, and R. W. Ledeen Induction of Calcium Influx through TRPC5 Channels by Cross-Linking of GM1 Ganglioside Associated with {alpha}5{beta}1 Integrin Initiates Neurite Outgrowth J. Neurosci., July 11, 2007; 27(28): 7447 - 7458. [Abstract] [Full Text] [PDF]

				A. Woronowicz, S. R. Amith, V. W Davis, P. Jayanth, K. De Vusser, W. Laroy, R. Contreras, S. O Meakin, and M. R Szewczuk Trypanosome trans-sialidase mediates neuroprotection against oxidative stress, serum/glucose deprivation, and hypoxia-induced neurite retraction in Trk-expressing PC12 cells Glycobiology, July 1, 2007; 17(7): 725 - 734. [Abstract] [Full Text] [PDF]

				A. Woronowicz, S. R. Amith, K. De Vusser, W. Laroy, R. Contreras, S. Basta, and M. R. Szewczuk Dependence of neurotrophic factor activation of Trk tyrosine kinase receptors on cellular sialidase Glycobiology, January 1, 2007; 17(1): 10 - 24. [Abstract] [Full Text] [PDF]

				S. Ueno, S. Saito, T. Wada, K. Yamaguchi, M. Satoh, Y. Arai, and T. Miyagi Plasma Membrane-associated Sialidase Is Up-regulated in Renal Cell Carcinoma and Promotes Interleukin-6-induced Apoptosis Suppression and Cell Motility J. Biol. Chem., March 24, 2006; 281(12): 7756 - 7764. [Abstract] [Full Text] [PDF]

				N. Papini, L. Anastasia, C. Tringali, G. Croci, R. Bresciani, K. Yamaguchi, T. Miyagi, A. Preti, A. Prinetti, S. Prioni, et al. The Plasma Membrane-associated Sialidase MmNEU3 Modifies the Ganglioside Pattern of Adjacent Cells Supporting Its Involvement in Cell-to-Cell Interactions J. Biol. Chem., April 23, 2004; 279(17): 16989 - 16995. [Abstract] [Full Text] [PDF]

				P. M. Crespo, A. R. Zurita, and J. L. Daniotti Effect of Gangliosides on the Distribution of a Glycosylphosphatidylinositol-anchored Protein in Plasma Membrane from Chinese Hamster Ovary-K1 Cells J. Biol. Chem., November 15, 2002; 277(47): 44731 - 44739. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help