QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (17) Citing Articles via Google Scholar Google Scholar Articles by O'Brien, J. A. Articles by Berger, A. J. Search for Related Content PubMed PubMed Citation Articles by O'Brien, J. A. Articles by Berger, A. J.

 Previous Article  |  Next Article 

The Journal of Neuroscience, November 1, 2001, 21(21):8482-8494

The Nonuniform Distribution of the GABA_A Receptor 1 Subunit Influences Inhibitory Synaptic Transmission to Motoneurons within a Motor Nucleus

Jennifer A. O'Brien and Albert J. Berger

Department of Physiology and Biophysics, University of Washington, Seattle, Washington 98195-7290

Using immunohistochemistry we studied the distribution of GABA_A and glycine receptor 1 subunits in the rat hypoglossal nucleus during postnatal development. In the neonate [postnatal day (P) 1-3] and adult nucleus (P28-30), GABA_A receptor 1 subunit labeling was relatively modest. However, in the juvenile nucleus (P9-13), labeling was strong in the ventrolateral region and moderate in the dorsal region. Glycine receptor 1 subunit labeling was strong and uniform in the juvenile and adult nucleus and absent in the neonate nucleus. GABA and glycine neurotransmitter labeling was uniform throughout the neonatal and juvenile nucleus. To study the functional consequences of this regional differential GABA_A receptor 1 subunit distribution, we voltage clamped juvenile hypoglossal motoneurons (HMs) from the ventrolateral and dorsal regions and recorded spontaneous miniature IPSCs (mIPSCs). Pure GABAergic events had slower decay times than glycinergic events. Although pure GABAergic and glycinergic decay times did not differ depending on HM location, the decays of mixed mIPSCs from ventrolateral HMs, recorded without GABA_A and glycine receptor antagonists, had significantly slower decays than mIPSCs from dorsal HMs. Focally applied GABA and glycine onto outside-out patches revealed that the GABAergic to glycinergic peak current amplitude ratio was larger for patches from ventrolateral HMs compared with dorsal HMs. Dual component mIPSCs, presumably caused by co-release of GABA and glycine, were recorded more frequently in the ventrolateral nucleus. These data suggest that the number of synapses using GABA_A receptor-mediated transmission is greater on ventrolateral HMs than dorsal HMs, demonstrating a nonuniformity of synaptic function within a defined motor nucleus.

Key words: glycine receptor; glycine; GABA; synaptic transmission; immunohistochemistry; GABA_A receptor; inhibition; hypoglossal motoneurons; hypoglossal nucleus; dorsal motor nucleus of vagus; brainstem

---

Copyright © 2001 Society for Neuroscience  0270-6474/01/21218482-13$05.00/0

This article has been cited by other articles:

				A. J. Camp, R. J. Callister, and A. M. Brichta Inhibitory Synaptic Transmission Differs in Mouse Type A and B Medial Vestibular Nucleus Neurons In Vitro J Neurophysiol, May 1, 2006; 95(5): 3208 - 3218. [Abstract] [Full Text] [PDF]

				G. P. Dugue, A. Dumoulin, A. Triller, and S. Dieudonne Target-Dependent Use of Coreleased Inhibitory Transmitters at Central Synapses J. Neurosci., July 13, 2005; 25(28): 6490 - 6498. [Abstract] [Full Text] [PDF]

				R. Lim, P. Hoang, and A. J. Berger Blockade of Glycine Transporter-1 (GLYT-1) Potentiates NMDA Receptor-Mediated Synaptic Transmission in Hypoglossal Motorneurons J Neurophysiol, October 1, 2004; 92(4): 2530 - 2537. [Abstract] [Full Text] [PDF]

				S. A. Saywell and J. L. Feldman Dynamic interactions of excitatory and inhibitory inputs in hypoglossal motoneurones: respiratory phasing and modulation by PKA J. Physiol., February 1, 2004; 554(3): 879 - 889. [Abstract] [Full Text] [PDF]

				B A Graham, P R Schofield, P Sah, and R J Callister Altered inhibitory synaptic transmission in superficial dorsal horn neurones in spastic and oscillator mice J. Physiol., September 15, 2003; 551(3): 905 - 916. [Abstract] [Full Text] [PDF]

				J. Y. Sebe, E. D. Eggers, and A. J. Berger Differential Effects of Ethanol on GABAA and Glycine Receptor-Mediated Synaptic Currents in Brain Stem Motoneurons J Neurophysiol, August 1, 2003; 90(2): 870 - 875. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help