QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (67) Citing Articles via Google Scholar Google Scholar Articles by Peunova, N. Articles by Enikolopov, G. Search for Related Content PubMed PubMed Citation Articles by Peunova, N. Articles by Enikolopov, G. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB NITRIC OXIDE

 Previous Article  |  Next Article 

The Journal of Neuroscience, November 15, 2001, 21(22):8809-8818

Nitric Oxide Is an Essential Negative Regulator of Cell Proliferation in Xenopus Brain

Natalia Peunova, Vladimir Scheinker, Hollis Cline, and Grigori Enikolopov

Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724

Mechanisms controlling the transition of a neural precursor cell from proliferation to differentiation during brain development determine the distinct anatomical features of the brain. Nitric oxide (NO) may mediate such a transition, because it can suppress DNA synthesis and cell proliferation. We cloned the gene encoding the neuronal isoform of Xenopus NO synthase (XNOS) and found that in the developing brain of Xenopus tadpoles, a zone of XNOS-expressing cells lies adjacent to the zone of dividing neuronal precursors. Exogenous NO, supplied to the tadpole brain in vivo, decreased the number of proliferating cells and the total number of cells in the optic tectum. Conversely, inhibition of NOS activity in vivo increased the number of proliferating cells and the total number of cells in the optic tectum. NOS inhibition yielded larger brains with grossly perturbed organization. Our results indicate that NO is an essential negative regulator of neuronal precursor proliferation during vertebrate brain development.

Key words: nitric oxide; Xenopus; differentiation; proliferation; brain; optic tectum; neuron; neuronal precursors

---

Copyright © 2001 Society for Neuroscience  0270-6474/01/21228809-10$05.00/0

This article has been cited by other articles:

				O. Hoffmann, C. Mahrhofer, N. Rueter, D. Freyer, B. Bert, H. Fink, and J. R. Weber Pneumococcal Cell Wall-Induced Meningitis Impairs Adult Hippocampal Neurogenesis Infect. Immun., September 1, 2007; 75(9): 4289 - 4297. [Abstract] [Full Text] [PDF]

				L. Coen, K. Le Blay, I. Rowe, and B. A. Demeneix Caspase-9 regulates apoptosis/proliferation balance during metamorphic brain remodeling in Xenopus PNAS, May 15, 2007; 104(20): 8502 - 8507. [Abstract] [Full Text] [PDF]

				R. Covacu, A. I. Danilov, B. S. Rasmussen, K. Hallen, M. C. Moe, A. Lobell, C. B. Johansson, M. A. Svensson, T. Olsson, and L. Brundin Nitric Oxide Exposure Diverts Neural Stem Cell Fate from Neurogenesis Towards Astrogliogenesis Stem Cells, December 1, 2006; 24(12): 2792 - 2800. [Abstract] [Full Text] [PDF]

				E. Ciani, V. Calvanese, C. Crochemore, R. Bartesaghi, and A. Contestabile Proliferation of cerebellar precursor cells is negatively regulated by nitric oxide in newborn rat J. Cell Sci., August 1, 2006; 119(15): 3161 - 3170. [Abstract] [Full Text] [PDF]

				C. Estrada and M. Murillo-Carretero Nitric Oxide and Adult Neurogenesis in Health and Disease Neuroscientist, August 1, 2005; 11(4): 294 - 307. [Abstract] [PDF]

				E. Ciani, S. Severi, A. Contestabile, R. Bartesaghi, and A. Contestabile Nitric oxide negatively regulates proliferation and promotes neuronal differentiation through N-Myc downregulation J. Cell Sci., September 15, 2004; 117(20): 4727 - 4737. [Abstract] [Full Text] [PDF]

				B. Holmqvist, B. Ellingsen, J. Forsell, I. Zhdanova, and P. Alm The early ontogeny of neuronal nitric oxide synthase systems in the zebrafish J. Exp. Biol., February 22, 2004; 207(6): 923 - 935. [Abstract] [Full Text] [PDF]

				D. Saur, J.-M. Vanderwinden, B. Seidler, R. M. Schmid, M.-H. De Laet, and H.-D. Allescher Single-nucleotide promoter polymorphism alters transcription of neuronal nitric oxide synthase exon 1c in infantile hypertrophic pyloric stenosis PNAS, February 10, 2004; 101(6): 1662 - 1667. [Abstract] [Full Text] [PDF]

				B. Moreno-Lopez, C. Romero-Grimaldi, J. A. Noval, M. Murillo-Carretero, E. R. Matarredona, and C. Estrada Nitric Oxide Is a Physiological Inhibitor of Neurogenesis in the Adult Mouse Subventricular Zone and Olfactory Bulb J. Neurosci., January 7, 2004; 24(1): 85 - 95. [Abstract] [Full Text] [PDF]

				J. Hemish, N. Nakaya, V. Mittal, and G. Enikolopov Nitric Oxide Activates Diverse Signaling Pathways to Regulate Gene Expression J. Biol. Chem., October 24, 2003; 278(43): 42321 - 42329. [Abstract] [Full Text] [PDF]

				M. A. Packer, Y. Stasiv, A. Benraiss, E. Chmielnicki, A. Grinberg, H. Westphal, S. A. Goldman, and G. Enikolopov Nitric oxide negatively regulates mammalian adult neurogenesis PNAS, August 5, 2003; 100(16): 9566 - 9571. [Abstract] [Full Text] [PDF]

				B. Cong, J. Liu, and S. D. Tanksley Natural alleles at a tomato fruit size quantitative trait locus differ by heterochronic regulatory mutations PNAS, October 15, 2002; 99(21): 13606 - 13611. [Abstract] [Full Text] [PDF]

				S. W. Park, J. Li, H. H. Loh, and L.-N. Wei A Novel Signaling Pathway of Nitric Oxide on Transcriptional Regulation of Mouse kappa Opioid Receptor Gene J. Neurosci., September 15, 2002; 22(18): 7941 - 7947. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help