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The Journal of Neuroscience, November 15, 2001, 21(22):8830-8841

Progressive Neuronal and Motor Dysfunction in Mice Overexpressing the Serine Protease Inhibitor Protease Nexin-1 in Postmitotic Neurons

Marita Meins¹, Petra Piosik¹, Nicole Schaeren-Wiemers¹, Stefania Franzoni¹, Edgardo Troncoso², Jozsef Z. Kiss², Christian Brösamle³, Martin E. Schwab³, Zoltán Molnár⁴, and Denis Monard¹

¹ Friedrich Miescher-Institut, CH-4058 Basel, Switzerland, ² University of Geneva Medical School, Department of Morphology, CH-1211 Geneva, Switzerland, ³ Brain Research Institute, University of Zürich and Eidgenössische Technische Hochschule Zürich, CH-8057 Zürich, Switzerland, and ⁴ Institute of Cellular Biology and Morphology, CH-1005 Lausanne, Switzerland

Perturbation of the homeostasis between proteases and their inhibitors has been associated with lesion-induced or degenerative neuronal changes. Protease nexin-1 (PN-1), a secreted serine protease inhibitor, is constitutively expressed in distinct neuronal cell populations of the adult CNS. In an earlier study we showed that transgenic mice with ectopic or increased expression of PN-1 in postnatal neurons have altered synaptic transmission. Here these mice are used to examine the impact of an extracellular proteolytic imbalance on long-term neuronal function. These mice develop disturbances in motor behavior from 12 weeks on, with some of the histopathological changes described in early stages of human motor neuron disease, and neurogenic muscle atrophy in old age. In addition, sensorimotor integration, measured by epicranial multichannel recording of sensory evoked potentials, is impaired. Our results suggest that axonal dysfunction rather than cell death underlies these phenotypes. In particular, long projecting neurons, namely cortical layer V pyramidal and spinal motor neurons, show an age-dependent vulnerability to PN-1 overexpression. These mice can serve to study early stages of in vivo neuronal dysfunction not yet associated with cell loss.

Key words: PN-1; motor behavior; motor neuron disease; proteolytic inhibition; transgenic mice; layer V pyramidal cells; muscle atrophy; axonopathy

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Copyright © 2001 Society for Neuroscience  0270-6474/01/21228830-12$05.00/0

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