 |
||||
|
||||
|
||||
|
||||
|
||||
Previous Article | Next Article
The Journal of Neuroscience, November 15, 2001, 21(22):8895-8905
Synchronous Oscillatory Activity in Immature Cortical Network Is Driven by GABAergic Preplate Neurons
Thomas Voigt, Thoralf Opitz, and Ana D. de Lima Otto-von-Guericke Universität, Medizinische Fakultät, Institut für Physiologie, 39120 Magdeburg, Germany
Neurons dissociated from embryonic cerebral rat cortex form a differentiated network of synaptic connections and develop synchronous oscillatory network activity with the beginning of the second week in culture. During an initial phase lasting 3-4 d, synchronous calcium transients can be blocked completely by either CNQX or bicuculline, showing that both glutamatergic and GABAergic neurons are required for the generation of this form of activity. By manipulating dissociation and growth conditions, cultures containing different populations of GABAergic neurons were obtained. These cultures revealed that a distinct population of large GABAergic neurons is a key element in the generation of synchronous oscillatory network activity. A minimal number of two large GABAergic neurons per square millimeter are required for the occurrence of synchronous activity. Changes in the density of all other types of GABAergic or non-GABAergic neurons has no influence on the synchronous activity. Electron microscopic analysis shows that the large GABAergic neurons form an interconnected network. Exceptionally high somatodendritic innervation and extended axonal arborization enable these neurons to collect electric network activity and to distribute it effectively throughout the neuronal network. Additional experiments indicated that most neurons developing in culture to large GABAergic neurons are derived from the primordial plexiform layer and reside in the subplate at the time of birth. We suggest that they function as an integrating element that synchronizes neuronal activity during early cortical development by collecting incoming extrinsic and intrinsic signals and distributing them effectively throughout the developing cortical plate.
Key words: development; cerebral cortex; calcium; synchronous activity; GABA; subplate
Copyright © 2001 Society for Neuroscience 0270-6474/01/21228895-11$05.00/0
This article has been cited by other articles:
K. Kirmse, A. Dvorzhak, C. Henneberger, R. Grantyn, and S. Kirischuk
Cajal Retzius cells in the mouse neocortex receive two types of pre- and postsynaptically distinct GABAergic inputs
J. Physiol., December 15, 2007; 585(3): 881 - 895.
[Abstract] [Full Text] [PDF]
Y. Ben-Ari, J.-L. Gaiarsa, R. Tyzio, and R. Khazipov
GABA: A Pioneer Transmitter That Excites Immature Neurons and Generates Primitive Oscillations
Physiol Rev, October 1, 2007; 87(4): 1215 - 1284.
[Abstract] [Full Text] [PDF]
P. N Hunt, A. K McCabe, and M. M Bosma
Midline serotonergic neurones contribute to widespread synchronized activity in embryonic mouse hindbrain
J. Physiol., August 1, 2005; 566(3): 807 - 819.
[Abstract] [Full Text] [PDF]
W. J. Moody and M. M. Bosma
Ion Channel Development, Spontaneous Activity, and Activity-Dependent Development in Nerve and Muscle Cells
Physiol Rev, July 1, 2005; 85(3): 883 - 941.
[Abstract] [Full Text] [PDF]
C. Wyart, S. Cocco, L. Bourdieu, J.-F. Leger, C. Herr, and D. Chatenay
Dynamics of Excitatory Synaptic Components in Sustained Firing at Low Rates
J Neurophysiol, June 1, 2005; 93(6): 3370 - 3380.
[Abstract] [Full Text] [PDF]
T. Voigt, T. Opitz, and A. D. de Lima
Activation of Early Silent Synapses by Spontaneous Synchronous Network Activity Limits the Range of Neocortical Connections
J. Neurosci., May 4, 2005; 25(18): 4605 - 4615.
[Abstract] [Full Text] [PDF]
R. Corlew, M. M Bosma, and W. J Moody
Spontaneous, synchronous electrical activity in neonatal mouse cortical neurones
J. Physiol., October 15, 2004; 560(2): 377 - 390.
[Abstract] [Full Text] [PDF]
D. Eytan, N. Brenner, and S. Marom
Selective Adaptation in Networks of Cortical Neurons
J. Neurosci., October 15, 2003; 23(28): 9349 - 9356.
[Abstract] [Full Text] [PDF]
Q. Sang and S.-S. Tan
Contact-associated Neurite Outgrowth and Branching of Immature Cortical Interneurons
Cereb Cortex, June 1, 2003; 13(6): 677 - 683.
[Abstract] [Full Text] [PDF]
H. Gozlan and Y. Ben-Ari
Interneurons are the Source and the Targets of the First Synapses Formed in the Rat Developing Hippocampal Circuit
Cereb Cortex, June 1, 2003; 13(6): 684 - 692.
[Abstract] [Full Text] [PDF]
I. L. Hanganu, W. Kilb, and H. J. Luhmann
Functional Synaptic Projections onto Subplate Neurons in Neonatal Rat Somatosensory Cortex
J. Neurosci., August 15, 2002; 22(16): 7165 - 7176.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|