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Previous Article | Next Article
The Journal of Neuroscience, December 1, 2001, 21(23):9246-9254
Rats Expressing Human Cytosolic Copper-Zinc Superoxide Dismutase Transgenes with Amyotrophic Lateral Sclerosis: Associated Mutations Develop Motor Neuron Disease
Makiko Nagai1, Masashi Aoki1, Ichiro Miyoshi2, Masaaki Kato1, Piera Pasinelli3, Noriyuki Kasai2, Robert H. Brown Jr3, and Yasuto Itoyama1 1 Department of Neuroscience, Division of Neurology, 2 Institute for Experimental Animals, Tohoku University Graduate School of Medicine, Sendai 980-8574, Japan, and 3 Day Neuromuscular Research Laboratory, Massachusetts General Hospital, Charlestown, Massachusetts 02129
Some cases of familial amyotrophic lateral sclerosis (ALS) are caused by mutations in the gene encoding cytosolic, copper-zinc superoxide dismutase (SOD1). We report here that rats that express a human SOD1 transgene with two different ALS-associated mutations (G93A and H46R) develop striking motor neuron degeneration and paralysis. As in the human disease and transgenic ALS mice, pathological analysis demonstrates selective loss of motor neurons in the spinal cords of these transgenic rats. In spinal cord tissues, this is accompanied by activation of apoptotic genes known to be activated by mutant SOD1 protein in vitro and in vivo. These animals provide additional support for the proposition that motor neuron death in SOD1-related ALS reflects one or more acquired, neurotoxic properties of the mutant SOD1 protein. The larger size of this rat model as compared with the ALS mice will facilitate studies involving manipulations of spinal fluid (implantation of intrathecal catheters for chronic therapeutic studies; CSF sampling) and spinal cord (e.g., direct administration of viral- and cell-mediated therapies).
Key words: familial amyotrophic lateral sclerosis; copper-zinc SOD; SOD1; transgenic rats; glutamate; caspase
Copyright © 2001 Society for Neuroscience 0270-6474/01/21239246-09$05.00/0
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