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The Journal of Neuroscience, February 1, 2001, 21(3):1047-1055

Elevation of Nerve Growth Factor and Antisense Knockdown of TrkA Receptor during Contextual Memory Consolidation

Nancy J. Woolf¹, Amy M. Milov¹, Erik S. Schweitzer², and Ali Roghani³

¹ Department of Psychology and ² Brain Research Institute, University of California at Los Angeles, Los Angeles, California 90095, and ³ Department of Pharmacology, Texas Tech University Health Sciences Center, Lubbock, Texas 79430

We report here a series of experiments establishing a role for nerve growth factor and its high-affinity receptor TrkA in contextual memory consolidation. In all experiments, we trained rats in a novel chamber using tone and shock. Our first experiment revealed that endogenous nerve growth factor (NGF) increases in the hippocampus at a critical time during consolidation that occurs 1 week after training. NGF levels at other intervals (24 hr and 2 and 4 weeks after training) did not differ from those of naive control animals. In our second experiment, we blocked effects that NGF has at 1 week after training by infusing antisense TrkA phosphorothioate DNA oligonucleotide. Reduction of septohippocampal TrkA receptor expression selectively impaired memory consolidation for context but not for tone. Animals with antisense TrkA oligonucleotide infused into the medial septal area or CA1 of the hippocampus froze less when placed in the training chamber than did animals infused with inactive randomized oligonucleotide. At 4 weeks after training, antisense TrkA oligonucleotide had no effect on freezing. Third, we correlated levels of freezing with choline acetyltransferase (ChAT) and vesicular acetylcholine transporter (VAChT) immunohistochemistry. Antisense TrkA infused into CA1 of the hippocampus reduced cell body cross-sectional area for cholinergic cells in the medial septal area and decreased the density of hippocampal terminals labeled for ChAT and VAChT proteins. Cholinergic cell body measurements were significantly correlated with freezing. Taken together, these results indicate a role for nerve growth factor acting via the TrkA receptor on ChAT and VAChT proteins in contextual memory consolidation.

Key words: neurotrophins; Pavlovian conditioning; neural plasticity; hippocampus; DNA oligonucleotides; choline acetyltransferase; vesicular acetylcholine transporter

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Copyright © 2001 Society for Neuroscience  0270-6474/01/2131047-09$05.00/0

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