WWW.JNEUROSCI.ORG
-
The Journal of Neuroscience
QUICK SEARCH:   [advanced]


     
-


HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an eLetter
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (28)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ralston, E.
Right arrow Articles by Lømo, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ralston, E.
Right arrow Articles by Lømo, T.

 Previous Article  |  Next Article 

The Journal of Neuroscience, February 1, 2001, 21(3):875-883

Golgi Complex, Endoplasmic Reticulum Exit Sites, and Microtubules in Skeletal Muscle Fibers Are Organized by Patterned Activity

Evelyn Ralston1, Thorkil Ploug2, John Kalhovde3, and Terje Lømo3

1 Laboratory of Neurobiology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892-4062, 2 Copenhagen Muscle Research Centre, Department of Medical Physiology, Panum Institute, Copenhagen N, DK-2200 Denmark, and 3 Department of Physiology, University of Oslo, Blindern N-0317, Norway

The Golgi complex of skeletal muscle fibers is made of thousands of dispersed elements. The distributions of these elements and of the microtubules they associate with differ in fast compared with slow and in innervated compared with denervated fibers. To investigate the role of muscle impulse activity, we denervated fast extensor digitorum longus (EDL) and slow soleus (SOL) muscles of adult rats and stimulated them directly with patterns that resemble the impulse patterns of normal fast EDL (25 pulses at 150 Hz every 15 min) and slow SOL (200 pulses at 20 Hz every 30 sec) motor units. After 2 weeks of denervation plus stimulation, peripheral and central regions of muscle fibers were examined by immunofluorescence microscopy with regard to density and distribution of Golgi complex, microtubules, glucose transporter GLUT4, centrosomes, and endoplasmic reticulum exit sites. In extrajunctional regions, fast pattern stimulation preserved normal fast characteristics of all markers in EDL type IIB/IIX fibers, although inducing changes toward the fast phenotype in originally slow type I SOL fibers, such as a 1.5-fold decrease of the density of Golgi elements at the fiber surface. Slow pattern stimulation had converse effects such as a 2.2-fold increase of the density of Golgi elements at the EDL fiber surface. In junctional regions, where fast and slow fibers are similar, both stimulation patterns prevented a denervation-induced accumulation of GLUT4. The results indicate that patterns of muscle impulse activity, as normally imposed by motor neurons, play a major role in regulating the organization of Golgi complex and related proteins in the extrajunctional region of muscle fibers.

Key words: muscle; Golgi complex; microtubules; plasticity; patterned activity; denervation

Copyright © 2001 Society for Neuroscience  0270-6474/01/213875-09$05.00/0


This article has been cited by other articles:


Home page
 DiabetesHome page
H. P.M. M. Lauritzen, T. Ploug, H. Ai, M. Donsmark, C. Prats, and H. Galbo
Denervation and High-Fat Diet Reduce Insulin Signaling in T-Tubules in Skeletal Muscle of Living Mice
Diabetes, January 1, 2008; 57(1): 13 - 23.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
O. L Gervasio and W. D Phillips
Increased ratio of rapsyn to ACh receptor stabilizes postsynaptic receptors at the mouse neuromuscular synapse
J. Physiol., February 1, 2005; 562(3): 673 - 685.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
F. BALUSKA, D. VOLKMANN, and P. W. BARLOW
Eukaryotic Cells and their Cell Bodies: Cell Theory Revised
Ann. Bot., July 1, 2004; 94(1): 9 - 32.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
H. Ai, E. Ralston, H. P. M. M. Lauritzen, H. Galbo, and T. Ploug
Disruption of microtubules in rat skeletal muscle does not inhibit insulin- or contraction-stimulated glucose transport
Am J Physiol Endocrinol Metab, October 1, 2003; 285(4): E836 - E844.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
J C Bruusgaard, K Liestol, M Ekmark, K Kollstad, and K Gundersen
Number and spatial distribution of nuclei in the muscle fibres of normal mice studied in vivo
J. Physiol., September 1, 2003; 551(2): 467 - 478.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
L. L. Gough, J. Fan, S. Chu, S. Winnick, and K. A. Beck
Golgi Localization of Syne-1
Mol. Biol. Cell, June 1, 2003; 14(6): 2410 - 2424.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
F. J. Miana-Mena, S. Roux, J.-C. Benichou, R. Osta, and P. Brulet
Neuronal activity-dependent membrane traffic at the neuromuscular junction
PNAS, March 5, 2002; 99(5): 3234 - 3239.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
G. Bezakova and T. Lomo
Muscle Activity and Muscle Agrin Regulate the Organization of Cytoskeletal Proteins and Attached Acetylcholine Receptor (Achr) Aggregates in Skeletal Muscle Fibers
J. Cell Biol., June 25, 2001; 153(7): 1453 - 1464.
[Abstract] [Full Text] [PDF]


-
-

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help
-
Copyright 2009 by Society for Neuroscience ONLINE ISSN: 1529-2401
-