 |
|||||
|
|||||
|
|||||
|
|||||
|
|||||
Previous Article | Next Article
The Journal of Neuroscience, February 15, 2001, 21(4):1218-1227
Interaction of Stoned and Synaptotagmin in Synaptic Vesicle Endocytosis
Tim Fergestad and Kendal Broadie Department of Biology, University of Utah, Salt Lake City, Utah 84112-0840
The Drosophila dicistronic stoned locus encodes two distinctive presynaptic proteins, Stoned A (STNA) and Stoned B (STNB); STNA is a novel protein without homology to known synaptic proteins, and STNB contains a domain with homology to the endocytotic protein AP50. Both Stoned proteins colocalize precisely with endocytotic proteins including the AP2 complex and Dynamin in the "lattice network" characteristic of endocytotic domains in Drosophila presynaptic terminals. FM1-43 dye uptake studies in stoned mutants demonstrate a striking decrease in the size of the endo-exo-cycling synaptic vesicle pool and loss of spatial regulation of the vesicular recycling intermediates. Mutant synapses display a significant delay in vesicular membrane retrieval after depolarization and neurotransmitter release. These studies suggest that the Stoned proteins play a role in mediating synaptic vesicle endocytosis. We have documented previously a highly specific synaptic mislocalization and degradation of Synaptotagmin I in stoned mutants. Here we show that transgenic overexpression of Synaptotagmin I rescues stoned embryonic lethality and restores endocytotic recycling to normal levels. Furthermore, overexpression of Synaptotagmin I in otherwise wild-type animals results in increased synaptic dye uptake, indicating that Synaptotagmin I directly regulates the endo-exo-cycling synaptic vesicle pool size. In parallel with recent biochemical studies, this genetic analysis strongly suggests that Stoned proteins regulate the AP2-Synaptotagmin I interaction during synaptic vesicle endocytosis. We conclude that Stoned proteins control synaptic transmission strength by mediating the retrieval of Synaptotagmin I from the plasma membrane.
Key words: Drosophila; stoned; synaptotagmin; synapse; synaptic vesicle; endocytosis
Copyright © 2001 Society for Neuroscience 0270-6474/01/2141218-10$05.00/0
This article has been cited by other articles:
N. Vijayakrishnan, E. A. Woodruff III, and K. Broadie
Rolling blackout is required for bulk endocytosis in non-neuronal cells and neuronal synapses
J. Cell Sci., January 1, 2009; 122(1): 114 - 125.
[Abstract] [Full Text] [PDF]
M. Gu, K. Schuske, S. Watanabe, Q. Liu, P. Baum, G. Garriga, and E. M. Jorgensen
{micro}2 adaptin facilitates but is not essential for synaptic vesicle recycling in Caenorhabditis elegans
J. Cell Biol., December 2, 2008; 183(5): 881 - 892.
[Abstract] [Full Text] [PDF]
C. L. Gatto and K. Broadie
Temporal requirements of the fragile X mental retardation protein in the regulation of synaptic structure
Development, August 1, 2008; 135(15): 2637 - 2648.
[Abstract] [Full Text] [PDF]
A. A. Long, E. Kim, H.-T. Leung, E. Woodruff III, L. An, R. W. Doerge, W. L. Pak, and K. Broadie
Presynaptic Calcium Channel Localization and Calcium-Dependent Synaptic Vesicle Exocytosis Regulated by the Fuseless Protein
J. Neurosci., April 2, 2008; 28(14): 3668 - 3682.
[Abstract] [Full Text] [PDF]
N. Jung, M. Wienisch, M. Gu, J. B. Rand, S. L. Muller, G. Krause, E. M. Jorgensen, J. Klingauf, and V. Haucke
Molecular basis of synaptic vesicle cargo recognition by the endocytic sorting adaptor stonin 2
J. Cell Biol., December 31, 2007; 179(7): 1497 - 1510.
[Abstract] [Full Text] [PDF]
R. Renden and H. von Gersdorff
Synaptic Vesicle Endocytosis at a CNS Nerve Terminal: Faster Kinetics at Physiological Temperatures and Increased Endocytotic Capacity During Maturation
J Neurophysiol, December 1, 2007; 98(6): 3349 - 3359.
[Abstract] [Full Text] [PDF]
T.-W. Koh, V. I. Korolchuk, Y. P. Wairkar, W. Jiao, E. Evergren, H. Pan, Y. Zhou, K. J.T. Venken, O. Shupliakov, I. M. Robinson, et al.
Eps15 and Dap160 control synaptic vesicle membrane retrieval and synapse development
J. Cell Biol., July 10, 2007; 178(2): 309 - 322.
[Abstract] [Full Text] [PDF]
F.-D. Huang, E. Woodruff, R. Mohrmann, and K. Broadie
Rolling Blackout Is Required for Synaptic Vesicle Exocytosis
J. Neurosci., March 1, 2006; 26(9): 2369 - 2379.
[Abstract] [Full Text] [PDF]
H. Bao, R. W. Daniels, G. T. MacLeod, M. P. Charlton, H. L. Atwood, and B. Zhang
AP180 Maintains the Distribution of Synaptic and Vesicle Proteins in the Nerve Terminal and Indirectly Regulates the Efficacy of Ca2+-Triggered Exocytosis
J Neurophysiol, September 1, 2005; 94(3): 1888 - 1903.
[Abstract] [Full Text] [PDF]
D. R. Lazzell, R. Belizaire, P. Thakur, D. M. Sherry, and R. Janz
SV2B Regulates Synaptotagmin 1 by Direct Interaction
J. Biol. Chem., December 10, 2004; 279(50): 52124 - 52131.
[Abstract] [Full Text] [PDF]
J. Rohrbough, E. Rushton, L. Palanker, E. Woodruff, H. J. G. Matthies, U. Acharya, J. K. Acharya, and K. Broadie
Ceramidase Regulates Synaptic Vesicle Exocytosis and Trafficking
J. Neurosci., September 8, 2004; 24(36): 7789 - 7803.
[Abstract] [Full Text] [PDF]
K. Walther, M. K. Diril, N. Jung, and V. Haucke
Functional dissection of the interactions of stonin 2 with the adaptor complex AP-2 and synaptotagmin
PNAS, January 27, 2004; 101(4): 964 - 969.
[Abstract] [Full Text] [PDF]
P. S. Estes, T. C. Jackson, D. T. Stimson, S. Sanyal, L. E. Kelly, and M. Ramaswami
Functional Dissection of a Eukaryotic Dicistronic Gene: Transgenic stonedB, but Not stonedA, Restores Normal Synaptic Properties to Drosophila stoned Mutants
Genetics, September 1, 2003; 165(1): 185 - 196.
[Abstract] [Full Text] [PDF]
T. C. Sudhof
Synaptotagmins: Why So Many?
J. Biol. Chem., March 1, 2002; 277(10): 7629 - 7632.
[Full Text] [PDF]
M. Boehm and J. S. Bonifacino
Adaptins. The Final Recount
Mol. Biol. Cell, October 1, 2001; 12(10): 2907 - 2920.
[Abstract] [Full Text] [PDF]
H. Zhao and M. L. Nonet
A Conserved Mechanism of Synaptogyrin Localization
Mol. Biol. Cell, August 1, 2001; 12(8): 2275 - 2289.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|