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The Journal of Neuroscience, January 1, 2002, 22(1):294-304

Opposite Influences of Endogenous Dopamine D₁ and D₂ Receptor Activation on Activity States and Electrophysiological Properties of Striatal Neurons: Studies Combining In Vivo Intracellular Recordings and Reverse Microdialysis

Anthony R. West and Anthony A. Grace

Departments of Neuroscience and Psychiatry, Center for Neuroscience, University of Pittsburgh, Pittsburgh, Pennsylvania 15260

The tonic influence of dopamine D₁ and D₂ receptors on the activity of striatal neurons in vivo was investigated by performing intracellular recordings concurrently with reverse microdialysis in chloral hydrate-anesthetized rats. Striatal neurons were recorded in the vicinity of the microdialysis probe to assess their activity during infusions of artificial CSF (aCSF), the D₁ receptor antagonist SCH 23390 (10 µM), or the D₂ receptor antagonist eticlopride (20 µM). SCH 23390 perfusion decreased the excitability of striatal neurons exhibiting electrophysiological characteristics of spiny projection cells as evidenced by a decrease in the maximal depolarized membrane potential, a decrease in the amplitude of up-state events, and an increase in the intracellular current injection amplitude required to elicit an action potential. Conversely, a marked depolarization of up- and down-state membrane potential modes, a decrease in the amplitude of intracellular current injection required to elicit an action potential, and an increase in the number of spikes evoked by depolarizing current steps were observed in striatal neurons after local eticlopride infusion. A significant increase in maximal EPSP amplitude evoked by electrical stimulation of the prefrontal cortex was also observed during local eticlopride but not SCH 23390 infusion. These results indicate that in intact systems, ongoing dopaminergic neurotransmission exerts a powerful tonic modulatory influence on the up- and down-state membrane properties of striatal neurons and controls their excitability differentially via both D₁- and D₂-like receptors. Moreover, a significant component of D₂ receptor-mediated inhibition of striatal neuron activity in vivo occurs via suppression of excitatory synaptic transmission.

Key words: dopamine; D₁ receptor; D₂ receptor; striatum; striatal neurons; electrophysiology; in vivo intracellular recording; reverse microdialysis; rat

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Copyright © 2002 Society for Neuroscience  0270-6474/02/221294-11$05.00/0

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