QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Whitworth, T. L. Articles by Quick, M. W. Search for Related Content PubMed PubMed Citation Articles by Whitworth, T. L. Articles by Quick, M. W.

 Previous Article  |  Next Article 

The Journal of Neuroscience, 2001, 21:RC192:1-6

RAPID COMMUNICATION
Psychostimulants Differentially Regulate Serotonin Transporter Expression in Thalamocortical Neurons

Terri L. Whitworth, Laura C. Herndon, and Michael W. Quick

Department of Neurobiology, University of Alabama at Birmingham, Birmingham, Alabama 35294

5-HT transporters (SERTs) are transiently expressed in thalamocortical neurons during development, permitting these glutamatergic neurons to co-release 5-HT as a "borrowed" transmitter. The high level of SERT expression in these neurons is likely important in the serotonergic modulation of neocortical circuits and provides a system for examining endogenous SERT regulation. We tested the hypothesis that developmental expression of SERT in thalamocortical neurons is regulated by psychostimulants that are agonists and antagonists of SERT. Cultured thalamocortical neurons from embryonic day 18 rats were examined for SERT expression until P15. In untreated cultures, SERT protein levels peaked at postnatal day 3 (P3) and were absent by P10. Chronic treatment with SERT substrates (5-HT, 3,4-methylenedioxymethamphetamine) increased both peak SERT protein levels (fourfold) and the time course of SERT expression. SERT substrates also shifted the relative functional expression of SERT by redistributing intracellular SERT protein to the plasma membrane. The subcellular redistribution was prevented by PKC activators. SERT antagonists (e.g., fluoxetine, cocaine) reduced total SERT expression levels and the time course of SERT expression. These data (1) show that endogenous SERT is differentially regulated by 5-HT and psychostimulants, (2) indicate that SERT modulation occurs via changes in both total SERT protein levels and subcellular redistribution of the transporter, and (3) suggest that some of the actions of drugs of abuse in neocortical development may be attributable to alterations in SERT expression and concomitant changes in 5-HT signaling.

Key words: cortex; development; drug abuse; monoamine; regulation; uptake

---

Copyright © Society for Neuroscience  0270-6474//$05.00/0

This article has been cited by other articles:

				K. L. VanDeMark, M. Guizzetti, G. Giordano, and L. G. Costa The Activation of M1 Muscarinic Receptor Signaling Induces Neuronal Differentiation in Pyramidal Hippocampal Neurons J. Pharmacol. Exp. Ther., May 1, 2009; 329(2): 532 - 542. [Abstract] [Full Text] [PDF]

				L. D. Jayanthi, D. J. Samuvel, R. D. Blakely, and S. Ramamoorthy Evidence for Biphasic Effects of Protein Kinase C on Serotonin Transporter Function, Endocytosis, and Phosphorylation Mol. Pharmacol., June 1, 2005; 67(6): 2077 - 2087. [Abstract] [Full Text] [PDF]

				S. Seidel, E. A. Singer, H. Just, H. Farhan, P. Scholze, O. Kudlacek, M. Holy, K. Koppatz, P. Krivanek, M. Freissmuth, et al. Amphetamines Take Two to Tango: an Oligomer-Based Counter-Transport Model of Neurotransmitter Transport Explores the Amphetamine Action Mol. Pharmacol., January 1, 2005; 67(1): 140 - 151. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help