QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (19) Citing Articles via Google Scholar Google Scholar Articles by Zhou, Y. Articles by Levitan, I. B. Search for Related Content PubMed PubMed Citation Articles by Zhou, Y. Articles by Levitan, I. B.

 Previous Article  |  Next Article 

The Journal of Neuroscience, May 15, 2002, 22(10):3855-3863

Modulation of Drosophila Slowpoke Calcium-Dependent Potassium Channel Activity by Bound Protein Kinase A Catalytic Subunit

Yi Zhou, Jing Wang, Hua Wen, Olga Kucherovsky, and Irwin B. Levitan

Department of Neuroscience, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104

Drosophila Slowpoke (dSlo) calcium-dependent potassium channels bind directly to the catalytic subunit of cAMP-dependent protein kinase (PKAc). We demonstrate here that coexpression of PKAc with dSlo in mammalian cells results in a dramatic decrease of dSlo channel activity. This modulation requires catalytically active PKAc but is not mediated by phosphorylation of S942, the only PKA consensus site in the dSlo C-terminal domain. dSlo binds to free PKAc but not to the PKA holoenzyme that includes regulatory subunits and is inactive. Activators of endogenous PKA that stimulate dSlo phosphorylation, but do not produce detectable PKAc binding to dSlo, do not modulate channel function. Furthermore, the catalytically inactive PKAc mutant does bind to dSlo but does not modulate channel activity. These results are consistent with the hypothesis that both binding of active PKAc to dSlo and phosphorylation of dSlo or some other protein are necessary for channel modulation.

Key words: Ca²⁺-dependent K⁺ channel; dSlo; cAMP-dependent protein kinase; protein-protein interaction; phosphorylation; whole-cell recording; modulation

---

Copyright © 2002 Society for Neuroscience  0270-6474/02/22103855-09$05.00/0

This article has been cited by other articles:

				S. Dai, D. D. Hall, and J. W. Hell Supramolecular Assemblies and Localized Regulation of Voltage-Gated Ion Channels Physiol Rev, April 1, 2009; 89(2): 411 - 452. [Abstract] [Full Text] [PDF]

				C. Yuan, R. J. O'Connell, A. Wilson, A. Z. Pietrzykowski, and S. N. Treistman Acute Alcohol Tolerance Is Intrinsic to the BKCa Protein, but Is Modulated by the Lipid Environment J. Biol. Chem., February 22, 2008; 283(8): 5090 - 5098. [Abstract] [Full Text] [PDF]

				C. K. Isacson, Q. Lu, R. H. Karas, and D. H. Cox RACK1 is a BKCa channel binding protein Am J Physiol Cell Physiol, April 1, 2007; 292(4): C1459 - C1466. [Abstract] [Full Text] [PDF]

				B. M. Hagen and K. M. Sanders Deglycosylation of the beta1-subunit of the BK channel changes its biophysical properties Am J Physiol Cell Physiol, October 1, 2006; 291(4): C750 - C756. [Abstract] [Full Text] [PDF]

				D. Wicher, J. Berlau, C. Walther, and A. Borst Peptidergic Counter-Regulation of Ca2+- and Na+-Dependent K+ Currents Modulates the Shape of Action Potentials in Neurosecretory Insect Neurons J Neurophysiol, January 1, 2006; 95(1): 311 - 322. [Abstract] [Full Text] [PDF]

				R. Lu, A. Alioua, Y. Kumar, M. Eghbali, E. Stefani, and L. Toro MaxiK channel partners: physiological impact J. Physiol., January 1, 2006; 570(1): 65 - 72. [Abstract] [Full Text] [PDF]

				M. T. Lin, L. D. Longo, W. J. Pearce, and D. A. Hessinger Ca2+-activated K+ channel-associated phosphatase and kinase activities during development Am J Physiol Heart Circ Physiol, July 1, 2005; 289(1): H414 - H425. [Abstract] [Full Text] [PDF]

				L. Tian, L. S. Coghill, H. McClafferty, S. H.-F. MacDonald, F. A. Antoni, P. Ruth, H.-G. Knaus, and M. J. Shipston Distinct stoichiometry of BKCa channel tetramer phosphorylation specifies channel activation and inhibition by cAMP-dependent protein kinase PNAS, August 10, 2004; 101(32): 11897 - 11902. [Abstract] [Full Text] [PDF]

				N. S. Magoski Regulation of an Aplysia Bag Cell Neuron Cation Channel by Closely Associated Protein Kinase A and a Protein Phosphatase J. Neurosci., July 28, 2004; 24(30): 6833 - 6841. [Abstract] [Full Text] [PDF]

				R. A. Baines Postsynaptic Protein Kinase A Reduces Neuronal Excitability in Response to Increased Synaptic Excitation in the Drosophila CNS J. Neurosci., September 24, 2003; 23(25): 8664 - 8672. [Abstract] [Full Text] [PDF]

				R. B. Renden and K. Broadie Mutation and Activation of Galpha s Similarly Alters Pre- and Postsynaptic Mechanisms Modulating Neurotransmission J Neurophysiol, May 1, 2003; 89(5): 2620 - 2638. [Abstract] [Full Text] [PDF]

				Y. Zhou, S. Reddy, H. Murrey, H. Fei, and I. B. Levitan Monomeric 14-3-3 Protein Is Sufficient to Modulate the Activity of the Drosophila Slowpoke Calcium-dependent Potassium Channel J. Biol. Chem., March 14, 2003; 278(12): 10073 - 10080. [Abstract] [Full Text] [PDF]

				L. Tian, L. S. Coghill, S. H.-F. MacDonald, D. L. Armstrong, and M. J. Shipston Leucine Zipper Domain Targets cAMP-dependent Protein Kinase to Mammalian BK Channels J. Biol. Chem., February 28, 2003; 278(10): 8669 - 8677. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help