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The Journal of Neuroscience, June 1, 2002, 22(11):4499-4508
Altered Levels of Gq Activity Modulate Axonal Pathfinding in
Drosophila
Anuradha
Ratnaparkhi,
Santanu
Banerjee, and
Gaiti
Hasan
National Center for Biological Sciences, Tata Institute of
Fundamental Research, Bangalore 560065, India
A majority of neurons that form the ventral nerve cord send out
long axons that cross the midline through anterior or posterior commissures. A smaller fraction extend longitudinally and never cross
the midline. The decision to cross the midline is governed by a balance
of attractive and repulsive signals. We have explored the role of a
G-protein, G q, in altering this balance in
Drosophila. A splice variant of Gq,
dgq3, is expressed in early axonal
growth cones, which go to form the commissures in the
Drosophila embryonic CNS. Misexpression of a
gain-of-function transgene of dgq3
(AcGq3) leads to ectopic midline crossing. Analysis of
the AcGq3 phenotype in roundabout and
frazzled mutants shows that AcGq3 function is antagonistic to Robo signaling and requires Frazzled to promote ectopic
midline crossing. Our results show for the first time that a
heterotrimeric G-protein can affect the balance of attractive versus
repulsive cues in the growth cone and that it can function as a
component of signaling pathways that regulate axonal pathfinding.
Key words:
dgq; Robo; Frazzled; Netrins; G-protein; midline; axon guidance
Copyright © 2002 Society for Neuroscience 0270-6474/02/22114499-10$05.00/0
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