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The Journal of Neuroscience, July 1, 2002, 22(13):5287-5290

BRIEF COMMUNICATION
A Genetic Method for Selective and Quickly Reversible Silencing of Mammalian Neurons

Hilde A. E. Lechner, Edward S. Lein, and Edward M. Callaway

Systems Neurobiology Laboratories, The Salk Institute, La Jolla, California 92037

Genetic methods for neuronal silencing have great promise for allowing selective inactivation of specific cell types within complex neural systems. Present methods, however, are limited in their reversibility by the slow time scale (days) of transcriptional regulation. We report the rapid and reversible inactivation of mammalian cortical neurons expressing the insect G-protein-coupled receptor AlstR (none Drosophilanone allatostatin receptor) after application of its peptide ligand allatostatin (AL). The onset and reversal of inactivation could be achieved rapidly, within minutes. Moreover, the effects of AL were selective for AlstR-transfected neurons. The AlstR/AL system is therefore a promising genetic method for selective and quickly reversible silencing of neuronal activity.

Key words: AlstR; allatostatin; GIRK; neural silencing; insect receptor; cortical neurons

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Copyright © 2002 Society for Neuroscience  0270-6474/02/22135287-04$05.00/0

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