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The Journal of Neuroscience, July 1, 2002, 22(13):5287-5290
BRIEF COMMUNICATION
A Genetic Method for Selective and Quickly Reversible Silencing
of Mammalian Neurons
Hilde A. E.
Lechner,
Edward
S.
Lein, and
Edward M.
Callaway
Systems Neurobiology Laboratories, The Salk Institute, La Jolla,
California 92037
Genetic methods for neuronal silencing have great promise for
allowing selective inactivation of specific cell types within complex
neural systems. Present methods, however, are limited in their
reversibility by the slow time scale (days) of transcriptional regulation. We report the rapid and reversible inactivation of mammalian cortical neurons expressing the insect G-protein-coupled receptor AlstR (none Drosophilanone allatostatin receptor) after application of its peptide ligand allatostatin (AL). The onset and
reversal of inactivation could be achieved rapidly, within minutes.
Moreover, the effects of AL were selective for AlstR-transfected neurons. The AlstR/AL system is therefore a promising genetic method
for selective and quickly reversible silencing of neuronal activity.
Key words:
AlstR; allatostatin; GIRK; neural silencing; insect
receptor; cortical neurons
Copyright © 2002 Society for Neuroscience 0270-6474/02/22135287-04$05.00/0
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