 |
||||
|
||||
|
||||
|
||||
Previous Article | Next Article
The Journal of Neuroscience, July 15, 2002, 22(14):6239-6246
Phrenic Long-Term Facilitation Requires Spinal Serotonin Receptor Activation and Protein Synthesis
Tracy. L. Baker-Herman and Gordon S. Mitchell Department of Comparative Biosciences and Center for Neuroscience, University of Wisconsin, Madison, Wisconsin 53706
Respiratory long-term facilitation (LTF) is a form of serotonin-dependent plasticity induced by intermittent hypoxia. LTF is manifested as a long-lasting increase in respiratory amplitude (and frequency) after the hypoxic episodes have ended. We tested the hypotheses that LTF of phrenic amplitude requires spinal serotonin receptor activation and spinal protein synthesis. A broad-spectrum serotonin receptor antagonist (methysergide) or protein synthesis inhibitors (emetine or cycloheximide) were injected intrathecally in the cervical spinal cord of anesthetized rats. Control rats, injected with vehicle (artificial CSF), exhibited an augmented phrenic burst amplitude after three 5 min episodes of hypoxia (78 ± 15% above baseline, 60 min after hypoxia; p < 0.05), indicating LTF. Pretreatment with methysergide, emetine, or cycloheximide attenuated or abolished phrenic LTF (20 ± 4, 0.2 ± 11, and 20 ± 2%, respectively; all p > 0.05). With protein synthesis inhibitors, phrenic LTF differed from control by 15 min after intermittent hypoxia. As an internal control against unintended drug distribution, we measured respiratory LTF in hypoglossal (XII) motor output. At 60 min after intermittent hypoxia, all treatment groups exhibited similar XII LTF (artificial CSF, 44 ± 10%; methysergide, 40 ± 5%; emetine, 35 ± 9%; and cycloheximide, 57 ± 29%; all p < 0.05), suggesting that drugs were restricted at effective doses to the spinal cord. We conclude that phrenic LTF requires spinal serotonin receptor activation and protein synthesis. Serotonin receptors on phrenic motoneuron dendrites may induce new protein synthesis, thereby giving rise to phrenic LTF.
Key words: control of breathing; serotonin; plasticity; intermittent hypoxia; motoneuron; long-term facilitation
Copyright © 2002 Society for Neuroscience 0270-6474/02/22146239-08$05.00/0
This article has been cited by other articles:
Q. Liu and M. T. T. Wong-Riley
Postnatal changes in the expression of serotonin 2A receptors in various brain stem nuclei of the rat
J Appl Physiol, June 1, 2008; 104(6): 1801 - 1808.
[Abstract] [Full Text] [PDF]
S. Mahamed and G. S. Mitchell
Simulated apnoeas induce serotonin-dependent respiratory long-term facilitation in rats
J. Physiol., April 15, 2008; 586(8): 2171 - 2181.
[Abstract] [Full Text] [PDF]
J. E. R. Wilkerson, I. Satriotomo, T. L. Baker-Herman, J. J. Watters, and G. S. Mitchell
Okadaic Acid-Sensitive Protein Phosphatases Constrain Phrenic Long-Term Facilitation after Sustained Hypoxia
J. Neurosci., March 12, 2008; 28(11): 2949 - 2958.
[Abstract] [Full Text] [PDF]
M. R. Hodges, G. J. Tattersall, M. B. Harris, S. D. McEvoy, D. N. Richerson, E. S. Deneris, R. L. Johnson, Z.-F. Chen, and G. B. Richerson
Defects in Breathing and Thermoregulation in Mice with Near-Complete Absence of Central Serotonin Neurons
J. Neurosci., March 5, 2008; 28(10): 2495 - 2505.
[Abstract] [Full Text] [PDF]
A. Tadjalli, J. Duffin, Y. M. Li, H. Hong, and J. Peever
Inspiratory activation is not required for episodic hypoxia-induced respiratory long-term facilitation in postnatal rats
J. Physiol., December 1, 2007; 585(2): 593 - 606.
[Abstract] [Full Text] [PDF]
N. V. Neverova, S. A. Saywell, L. J. Nashold, G. S. Mitchell, and J. L. Feldman
Episodic Stimulation of {alpha}1-Adrenoreceptors Induces Protein Kinase C-Dependent Persistent Changes in Motoneuronal Excitability
J. Neurosci., April 18, 2007; 27(16): 4435 - 4442.
[Abstract] [Full Text] [PDF]
S. Mahamed and G. S. Mitchell
Sleep Apnoea & Hypertension: Physiological bases for a causal relation: Is there a link between intermittent hypoxia-induced respiratory plasticity and obstructive sleep apnoea?
Exp Physiol, January 1, 2007; 92(1): 27 - 37.
[Abstract] [Full Text] [PDF]
T. E. Dick, Y.-H. Hsieh, N. Wang, and N. Prabhakar
Acute intermittent hypoxia increases both phrenic and sympathetic nerve activities in the rat
Exp Physiol, January 1, 2007; 92(1): 87 - 97.
[Abstract] [Full Text] [PDF]
C. J. Madden and S. F. Morrison
Serotonin potentiates sympathetic responses evoked by spinal NMDA
J. Physiol., December 1, 2006; 577(2): 525 - 537.
[Abstract] [Full Text] [PDF]
A. G. Zabka, G. S. Mitchell, and M. Behan
Conversion from testosterone to oestradiol is required to modulate respiratory long-term facilitation in male rats
J. Physiol., November 1, 2006; 576(3): 903 - 912.
[Abstract] [Full Text] [PDF]
A. K. Tryba, F. Pena, and J.-M. Ramirez
Gasping activity in vitro: a rhythm dependent on 5-HT2A receptors.
J. Neurosci., March 8, 2006; 26(10): 2623 - 2634.
[Abstract] [Full Text] [PDF]
B. Bariohay, B. Lebrun, E. Moyse, and A. Jean
Brain-Derived Neurotrophic Factor Plays a Role as an Anorexigenic Factor in the Dorsal Vagal Complex
Endocrinology, December 1, 2005; 146(12): 5612 - 5620.
[Abstract] [Full Text] [PDF]
B. L. Shay, M. Sawchuk, D. W. Machacek, and S. Hochman
Serotonin 5-HT2 Receptors Induce a Long-Lasting Facilitation of Spinal Reflexes Independent of Ionotropic Receptor Activity
J Neurophysiol, October 1, 2005; 94(4): 2867 - 2877.
[Abstract] [Full Text] [PDF]
M. McGuire, Y. Zhang, D. P. White, and L. Ling
Phrenic long-term facilitation requires NMDA receptors in the phrenic motonucleus in rats
J. Physiol., September 1, 2005; 567(2): 599 - 611.
[Abstract] [Full Text] [PDF]
F. J. Golder and G. S. Mitchell
Spinal Synaptic Enhancement with Acute Intermittent Hypoxia Improves Respiratory Function after Chronic Cervical Spinal Cord Injury
J. Neurosci., March 16, 2005; 25(11): 2925 - 2932.
[Abstract] [Full Text] [PDF]
A. G Zabka, G. S Mitchell, and M Behan
Ageing and gonadectomy have similar effects on hypoglossal long-term facilitation in male Fischer rats
J. Physiol., March 1, 2005; 563(2): 557 - 568.
[Abstract] [Full Text] [PDF]
S. C. Veasey, G. Zhan, P. Fenik, and D. Pratico
Long-Term Intermittent Hypoxia: Reduced Excitatory Hypoglossal Nerve Output
Am. J. Respir. Crit. Care Med., September 15, 2004; 170(6): 665 - 672.
[Abstract] [Full Text] [PDF]
L. C. McKay, W. A. Janczewski, and J. L. Feldman
Episodic hypoxia evokes long-term facilitation of genioglossus muscle activity in neonatal rats
J. Physiol., May 15, 2004; 557(1): 13 - 18.
[Abstract] [Full Text] [PDF]
J. H. Mateika, C. Mendello, D. Obeid, and M. S. Badr
Peripheral chemoreflex responsiveness is increased at elevated levels of carbon dioxide after episodic hypoxia in awake humans
J Appl Physiol, March 1, 2004; 96(3): 1197 - 1205.
[Abstract] [Full Text] [PDF]
M. McGuire, Y. Zhang, D. P. White, and L. Ling
Serotonin receptor subtypes required for ventilatory long-term facilitation and its enhancement after chronic intermittent hypoxia in awake rats
Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2004; 286(2): R334 - R341.
[Abstract] [Full Text] [PDF]
J. A. Neubauer and J. Sunderram
Oxygen-sensing neurons in the central nervous system
J Appl Physiol, January 1, 2004; 96(1): 367 - 374.
[Abstract] [Full Text] [PDF]
D. Gozal, S. R. Reeves, B. W. Row, J. J. Neville, S. Z. Guo, and A. J. Lipton
Respiratory Effects of Gestational Intermittent Hypoxia in the Developing Rat
Am. J. Respir. Crit. Care Med., June 1, 2003; 167(11): 1540 - 1547.
[Abstract] [Full Text] [PDF]
Y. D. Teng, M. Bingaman, A. M. Taveira-DaSilva, P. P. Pace, R. A. Gillis, and J. R. Wrathall
Serotonin 1A Receptor Agonists Reverse Respiratory Abnormalities in Spinal Cord-Injured Rats
J. Neurosci., May 15, 2003; 23(10): 4182 - 4189.
[Abstract] [Full Text] [PDF]
N.-W. Hu, H.-M. Zhang, X.-D. Hu, M.-T. Li, T. Zhang, L.-J. Zhou, and X.-G. Liu
Protein Synthesis Inhibition Blocks the Late-Phase LTP of C-Fiber Evoked Field Potentials in Rat Spinal Dorsal Horn
J Neurophysiol, May 1, 2003; 89(5): 2354 - 2359.
[Abstract] [Full Text] [PDF]
D. D. Fuller, S. M. Johnson, E. B. Olson Jr, and G. S. Mitchell
Synaptic Pathways to Phrenic Motoneurons Are Enhanced by Chronic Intermittent Hypoxia after Cervical Spinal Cord Injury
J. Neurosci., April 1, 2003; 23(7): 2993 - 3000.
[Abstract] [Full Text] [PDF]
C. M. Bocchiaro, S. A. Saywell, and J. L. Feldman
Dynamic Modulation of Inspiratory Drive Currents by Protein Kinase A and Protein Phosphatases in Functionally Active Motoneurons
J. Neurosci., February 15, 2003; 23(4): 1099 - 1103.
[Abstract] [Full Text] [PDF]
G. S. Mitchell and S. M. Johnson
Plasticity in Respiratory Motor Control: Invited Review: Neuroplasticity in respiratory motor control
J Appl Physiol, January 1, 2003; 94(1): 358 - 374.
[Abstract] [Full Text] [PDF]
R. W. Bavis and G. S. Mitchell
Plasticity in Respiratory Motor Control: Selected Contribution: Intermittent hypoxia induces phrenic long-term facilitation in carotid-denervated rats
J Appl Physiol, January 1, 2003; 94(1): 399 - 409.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|