 |
||||
|
||||
|
||||
|
||||
Previous Article | Next Article
The Journal of Neuroscience, August 15, 2002, 22(16):7055-7064
In Vivo Labeling of Parvalbumin-Positive Interneurons and Analysis of Electrical Coupling in Identified Neurons
Axel H. Meyer1, István Katona1, Maria Blatow1, Andrei Rozov2, and Hannah Monyer1 1 Department of Clinical Neurobiology, Interdisciplinary Center for Neurosciences, University of Heidelberg, 69120 Heidelberg, Germany, and 2 Department of Experimental Neurophysiology, Faculty of Earth and Life Science, Vrije University Amsterdam, De Boelelaan 1087 1081 HV Amsterdam, The Netherlands
GABAergic interneurons can pace the activity of principal cells and are thus critically involved in the generation of oscillatory and synchronous network activity. The specific role of various GABAergic subpopulations, however, has remained elusive. This is in part attributable to the scarcity of certain GABAergic neurons and the difficulty of identifying them in slices obtained from brain regions in which anatomical structures are not readily recognizable in the live preparation. To facilitate the functional analysis of GABAergic interneurons, we generated transgenic mice in which the enhanced green fluorescent protein (EGFP) was specifically expressed in parvalbumin-positive neurons. The high fidelity of expression obtained using bacterial artificial chromosome transgenes resulted in EGFP-labeled neurons in nearly all brain regions known to contain parvalbumin-expressing neurons. Immunocytochemical analysis showed that EGFP expression was primarily restricted to parvalbumin-positive cells. In addition to cell body labeling, EGFP expression was high enough in many neurons to enable the visualization of dendritic structures. With the help of these mice, we investigated the presence of electrical coupling between parvalbumin-positive cells in brain slices obtained from young and adult animals. In dentate gyrus basket cells, electrical coupling was found in slices from young [postnatal day 14 (P14)] and adult (P28 and P42) animals, but both strength and incidence of coupling decreased during development. However, electrical coupling between parvalbumin-positive multipolar cells in layer II/III of the neocortex remains unaltered during development. Yet another developmental profile of electrical coupling was found between layer II/III parvalbumin-positive cells and excitatory principal cells. Between these neurons, electrical coupling was found at P14 but not at P28. The results indicate that the presence and strength of electrical coupling is developmentally regulated with respect to brain area and cell type.
Key words: interneuron; GABA; parvalbumin; gap junction; EGFP; BAC
Copyright © 2002 Society for Neuroscience 0270-6474/02/22167055-10$05.00/0
This article has been cited by other articles:
M. Uematsu, Y. Hirai, F. Karube, S. Ebihara, M. Kato, K. Abe, K. Obata, S. Yoshida, M. Hirabayashi, Y. Yanagawa, et al.
Quantitative Chemical Composition of Cortical GABAergic Neurons Revealed in Transgenic Venus-Expressing Rats
Cereb Cortex, February 1, 2008; 18(2): 315 - 330.
[Abstract] [Full Text] [PDF]
A. Baude, C. Bleasdale, Y. Dalezios, P. Somogyi, and T. Klausberger
Immunoreactivity for the GABAA Receptor {alpha}1 Subunit, Somatostatin and Connexin36 Distinguishes Axoaxonic, Basket, and Bistratified Interneurons of the Rat Hippocampus
Cereb Cortex, September 1, 2007; 17(9): 2094 - 2107.
[Abstract] [Full Text] [PDF]
J. M. Wilson, D. A. Dombeck, M. Diaz-Rios, R. M. Harris-Warrick, and R. M. Brownstone
Two-Photon Calcium Imaging of Network Activity in XFP-Expressing Neurons in the Mouse
J Neurophysiol, April 1, 2007; 97(4): 3118 - 3125.
[Abstract] [Full Text] [PDF]
A. Devor, A. Trevelyan, and D. Kleinfeld
Is There a Common Origin to Surround-Inhibition as Seen Through Electrical Activity Versus Hemodynamic Changes? Focus on "Duration-Dependent Response in SI to Vibrotactile Stimulation in Squirrel Monkey"
J Neurophysiol, March 1, 2007; 97(3): 1880 - 1882.
[Full Text] [PDF]
A. R. Woodruff and P. Sah
Networks of Parvalbumin-Positive Interneurons in the Basolateral Amygdala
J. Neurosci., January 17, 2007; 27(3): 553 - 563.
[Abstract] [Full Text] [PDF]
V. Zsiros, I. Aradi, and G. Maccaferri
Propagation of postsynaptic currents and potentials via gap junctions in GABAergic networks of the rat hippocampus
J. Physiol., January 15, 2007; 578(2): 527 - 544.
[Abstract] [Full Text] [PDF]
Y. I. Verbny, F. Erdelyi, G. Szabo, and M. I. Banks
Properties of a Population of GABAergic Cells in Murine Auditory Cortex Weakly Excited by Thalamic Stimulation
J Neurophysiol, December 1, 2006; 96(6): 3194 - 3208.
[Abstract] [Full Text] [PDF]
A. R. Woodruff, H. Monyer, and P. Sah
GABAergic Excitation in the Basolateral Amygdala.
J. Neurosci., November 15, 2006; 26(46): 11881 - 11887.
[Abstract] [Full Text] [PDF]
T. Gallopin, H. Geoffroy, J. Rossier, and B. Lambolez
Cortical Sources of CRF, NKB, and CCK and Their Effects on Pyramidal Cells in the Neocortex
Cereb Cortex, October 1, 2006; 16(10): 1440 - 1452.
[Abstract] [Full Text] [PDF]
M. J. Rossner, J. Hirrlinger, S. P. Wichert, C. Boehm, D. Newrzella, H. Hiemisch, G. Eisenhardt, C. Stuenkel, O. von Ahsen, and K.-A. Nave
Global Transcriptome Analysis of Genetically Identified Neurons in the Adult Cortex
J. Neurosci., September 27, 2006; 26(39): 9956 - 9966.
[Abstract] [Full Text] [PDF]
I. Freiman, A. Anton, H. Monyer, M. J. Urbanski, and B. Szabo
Analysis of the effects of cannabinoids on identified synaptic connections in the caudate-putamen by paired recordings in transgenic mice
J. Physiol., September 15, 2006; 575(3): 789 - 806.
[Abstract] [Full Text] [PDF]
Y. Ma, H. Hu, A. S. Berrebi, P. H. Mathers, and A. Agmon
Distinct subtypes of somatostatin-containing neocortical interneurons revealed in transgenic mice.
J. Neurosci., May 10, 2006; 26(19): 5069 - 5082.
[Abstract] [Full Text] [PDF]
V. Zsiros and G. Maccaferri
Electrical Coupling between Interneurons with Different Excitable Properties in the Stratum Lacunosum-Moleculare of the Juvenile CA1 Rat Hippocampus
J. Neurosci., September 21, 2005; 25(38): 8686 - 8695.
[Abstract] [Full Text] [PDF]
M. A. Long, S. J. Cruikshank, M. J. Jutras, and B. W. Connors
Abrupt Maturation of a Spike-Synchronizing Mechanism in Neocortex
J. Neurosci., August 10, 2005; 25(32): 7309 - 7316.
[Abstract] [Full Text] [PDF]
M. Vandecasteele, J. Glowinski, and L. Venance
Electrical Synapses between Dopaminergic Neurons of the Substantia Nigra Pars Compacta
J. Neurosci., January 12, 2005; 25(2): 291 - 298.
[Abstract] [Full Text] [PDF]
T. Collin, M. Chat, M. G. Lucas, H. Moreno, P. Racay, B. Schwaller, A. Marty, and I. Llano
Developmental Changes in Parvalbumin Regulate Presynaptic Ca2+ Signaling
J. Neurosci., January 5, 2005; 25(1): 96 - 107.
[Abstract] [Full Text] [PDF]
L. Wittner, L. Eross, S. Czirjak, P. Halasz, T. F. Freund, and Zs. Magloczky
Surviving CA1 pyramidal cells receive intact perisomatic inhibitory input in the human epileptic hippocampus
Brain, January 1, 2005; 128(1): 138 - 152.
[Abstract] [Full Text] [PDF]
E. O. Mann, C. A. Radcliffe, and O. Paulsen
Hippocampal gamma-frequency oscillations: from interneurones to pyramidal cells, and back
J. Physiol., January 1, 2005; 562(1): 55 - 63.
[Abstract] [Full Text] [PDF]
M. Blatow, A. Caputi, and H. Monyer
Molecular diversity of neocortical GABAergic interneurones
J. Physiol., January 1, 2005; 562(1): 99 - 105.
[Abstract] [Full Text] [PDF]
T. Gloveli, T. Dugladze, S. Saha, H. Monyer, U. Heinemann, R. D. Traub, M. A. Whittington, and t. l. E. H. Buhl
Differential involvement of oriens/pyramidale interneurones in hippocampal network oscillations in vitro
J. Physiol., January 1, 2005; 562(1): 131 - 147.
[Abstract] [Full Text] [PDF]
Z. Henderson, A. Boros, G. Janzso, A. J. Westwood, H. Monyer, and K. Halasy
Somato-dendritic nicotinic receptor responses recorded in vitro from the medial septal diagonal band complex of the rodent
J. Physiol., January 1, 2005; 562(1): 165 - 182.
[Abstract] [Full Text] [PDF]
R. Bruzzone, S. G. Hormuzdi, M. T. Barbe, A. Herb, and H. Monyer
Pannexins, a family of gap junction proteins expressed in brain
PNAS, November 11, 2003; 100(23): 13644 - 13649.
[Abstract] [Full Text] [PDF]
Z. Chu, M. Galarreta, and S. Hestrin
Synaptic Interactions of Late-Spiking Neocortical Neurons in Layer 1
J. Neurosci., January 1, 2003; 23(1): 96 - 102.
[Abstract] [Full Text] [PDF]
M. Bartos, I. Vida, M. Frotscher, A. Meyer, H. Monyer, J. R. P. Geiger, and P. Jonas
Fast synaptic inhibition promotes synchronized gamma oscillations in hippocampal interneuron networks
PNAS, October 1, 2002; 99(20): 13222 - 13227.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|