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The Journal of Neuroscience, September 15, 2002, 22(18):7903-7912

Nicotinic 7 Receptor Clusters on Hippocampal GABAergic Neurons: Regulation by Synaptic Activity and Neurotrophins

Hideki Kawai^*, Wagner Zago^*, and Darwin K. Berg

Neurobiology Section, Biology Division, University of California, San Diego, La Jolla, California 92093-0357

Nicotinic acetylcholine receptors containing the 7 gene product are expressed at substantial levels in the hippocampus. Because of their specific locations and their high relative calcium permeability, the receptors not only mediate cholinergic transmission in the hippocampus but also influence signaling at noncholinergic synapses. We have used fluorescently labeled -bungarotoxin to image 7-containing receptors on hippocampal neurons and to examine their regulation in culture. The highest levels of staining for such receptors were most commonly found on GABAergic interneurons identified immunohistochemically. The receptors were distributed in clusters on the soma and dendrites and were localized in part at GABAergic synapses. A 3 d blockade of electrical activity with tetrodotoxin or NMDA receptors with APV dramatically reduced the proportion of GABAergic neurons expressing high levels of receptor staining and reduced the mean number of distinguishable receptor clusters on individual neurons. Blockade of either GABA_A receptors with bicuculline or nicotinic receptors with D-tubocurarine had no effect, although exposure to nicotine could increase the level of receptor staining. Anti-BDNF and anti-NGF antibodies produced decrements equivalent to those of tetrodotoxin and APV, whereas addition of BDNF and NGF each increased staining levels and increased the number of distinguishable receptor clusters on GABAergic neurons. The exogenous neurotrophins could not, however, overcome the effects of either tetrodotoxin or APV. The results indicate that both NMDA receptor activation and the neurotrophins BDNF and NGF are necessary to sustain the distribution patterns of 7-containing nicotinic receptors on GABAergic hippocampal neurons.

Key words: nicotinic; acetylcholine; receptors; hippocampal; BDNF; NGF; bungarotoxin

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^* H.K. and W.Z. contributed equally to this work.

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Copyright © 2002 Society for Neuroscience  0270-6474/02/22187903-10$05.00/0

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