 |
|||||
|
|||||
|
|||||
|
|||||
|
|||||
Previous Article | Next Article
The Journal of Neuroscience, September 15, 2002, 22(18):7948-7958
A New Activity of Doublecortin in Recognition of the Phospho-FIGQY Tyrosine in the Cytoplasmic Domain of Neurofascin
Krishnakumar Kizhatil, Yi-Xin Wu, Anindita Sen, and Vann Bennett Howard Hughes Medical Institute and Departments of Cell Biology, Biochemistry, and Neuroscience, Duke University Medical Center, Durham, North Carolina 27710
Doublecortin is a cytoplasmic protein mutated in the neuronal migration disorder X-linked lissencephaly. This study describes a novel activity of doublecortin in recognition of the FIGQY-phosphotyrosine motif present in the cytoplasmic domain of the L1 cell adhesion molecule neurofascin. Phospho-FIGQY-neurofascin (186 kDa) coimmunoprecipitated with doublecortin from detergent extracts of embryonic brain membranes, and this doublecortin-phospho-FIGQY neurofascin complex was disassociated by a synthetic phospho-FIGQY neurofascin peptide but not by a dephospho-FIGQY peptide. Doublecortin specifically recognized the phospho-FIGQY tyrosine in the context of a synthetic phospho-FIGQY neurofascin peptide and in phospho-FIGQY neurofascin isolated from cells treated with pervanadate. Mutations of doublecortin causing lissencephaly (R59H, D62N, and G253D) abolished binding to the phospho-FIGQY peptide and to phospho-FIGQY neurofascin. Finally, phospho-FIGQY neurofascin and doublecortin colocalize in developing axon tracts and in zones enriched in migrating neurons in the embryonic cerebral cortex. In the adult rostral migratory stream, doublecortin colocalizes in migrating neurons with a phospho-FIGQY bearing L1 CAM different from neurofascin. The finding that doublecortin associates with FIGQY-phosphorylated neurofascin provides the first connection of doublecortin with the plasma membrane and could be important for a function of doublecortin in directing neuronal migration.
Key words: doublecortin; phospho-FIGQY; neurofascin; L1 CAM; neuronal migration; axons
Copyright © 2002 Society for Neuroscience 0270-6474/02/22187948-11$05.00/0
This article has been cited by other articles:
N. Giagtzoglou, C. V. Ly, and H. J. Bellen
Cell Adhesion, the Backbone of the Synapse: "Vertebrate" and "Invertebrate" Perspectives
Cold Spring Harb Perspect Biol, October 1, 2009; 1(4): a003079 - a003079.
[Abstract] [Full Text] [PDF]
I. Tint, D. Jean, P. W. Baas, and M. M. Black
Doublecortin Associates with Microtubules Preferentially in Regions of the Axon Displaying Actin-Rich Protrusive Structures
J. Neurosci., September 2, 2009; 29(35): 10995 - 11010.
[Abstract] [Full Text] [PDF]
S. Zhou, K. Opperman, X. Wang, and L. Chen
unc-44 Ankyrin and stn-2 {gamma}-Syntrophin Regulate sax-7 L1CAM Function in Maintaining Neuronal Positioning in Caenorhabditis elegans
Genetics, November 1, 2008; 180(3): 1429 - 1443.
[Abstract] [Full Text] [PDF]
M. Buhusi, M. C. Schlatter, G. P. Demyanenko, R. Thresher, and P. F. Maness
L1 Interaction with Ankyrin Regulates Mediolateral Topography in the Retinocollicular Projection
J. Neurosci., January 2, 2008; 28(1): 177 - 188.
[Abstract] [Full Text] [PDF]
T. Boiko, M. Vakulenko, H. Ewers, C. C. Yap, C. Norden, and B. Winckler
Ankyrin-Dependent and -Independent Mechanisms Orchestrate Axonal Compartmentalization of L1 Family Members Neurofascin and L1/Neuron-Glia Cell Adhesion Molecule
J. Neurosci., January 17, 2007; 27(3): 590 - 603.
[Abstract] [Full Text] [PDF]
C. Kappeler, Y. Saillour, J.-P. Baudoin, F. P. D. Tuy, C. Alvarez, C. Houbron, P. Gaspar, G. Hamard, J. Chelly, C. Metin, et al.
Branching and nucleokinesis defects in migrating interneurons derived from doublecortin knockout mice
Hum. Mol. Genet., May 1, 2006; 15(9): 1387 - 1400.
[Abstract] [Full Text] [PDF]
E. Anderson, S. Maday, J. Sfakianos, M. Hull, B. Winckler, D. Sheff, H. Folsch, and I. Mellman
Transcytosis of NgCAM in epithelial cells reflects differential signal recognition on the endocytic and secretory pathways
J. Cell Biol., August 15, 2005; 170(4): 595 - 605.
[Abstract] [Full Text] [PDF]
J. D. Malhotra, V. Thyagarajan, C. Chen, and L. L. Isom
Tyrosine-phosphorylated and Nonphosphorylated Sodium Channel {beta}1 Subunits Are Differentially Localized in Cardiac Myocytes
J. Biol. Chem., September 24, 2004; 279(39): 40748 - 40754.
[Abstract] [Full Text] [PDF]
T. Tanaka, F. F. Serneo, C. Higgins, M. J. Gambello, A. Wynshaw-Boris, and J. G. Gleeson
Lis1 and doublecortin function with dynein to mediate coupling of the nucleus to the centrosome in neuronal migration
J. Cell Biol., June 7, 2004; 165(5): 709 - 721.
[Abstract] [Full Text] [PDF]
D. P. McEwen, L. S. Meadows, C. Chen, V. Thyagarajan, and L. L. Isom
Sodium Channel {beta}1 Subunit-mediated Modulation of Nav1.2 Currents and Cell Surface Density Is Dependent on Interactions with Contactin and Ankyrin
J. Biol. Chem., April 16, 2004; 279(16): 16044 - 16049.
[Abstract] [Full Text] [PDF]
O. D. Gil, T. Sakurai, A. E. Bradley, M. Y. Fink, M. R. Cassella, J. A. Kuo, and D. P. Felsenfeld
Ankyrin binding mediates L1CAM interactions with static components of the cytoskeleton and inhibits retrograde movement of L1CAM on the cell surface
J. Cell Biol., August 18, 2003; 162(4): 719 - 730.
[Abstract] [Full Text] [PDF]
M. Buhusi, B. R. Midkiff, A. M. Gates, M. Richter, M. Schachner, and P. F. Maness
Close Homolog of L1 Is an Enhancer of Integrin-mediated Cell Migration
J. Biol. Chem., June 27, 2003; 278(27): 25024 - 25031.
[Abstract] [Full Text] [PDF]
G. Friocourt, A. Koulakoff, P. Chafey, D. Boucher, F. Fauchereau, J. Chelly, and F. Francis
Doublecortin Functions at the Extremities of Growing Neuronal Processes
Cereb Cortex, June 1, 2003; 13(6): 620 - 626.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|