QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (22) Citing Articles via Google Scholar Google Scholar Articles by Miura, M. Articles by Kano, M. Search for Related Content PubMed PubMed Citation Articles by Miura, M. Articles by Kano, M. Pubmed/NCBI databases Gene GEO Profiles HomoloGene UniGene Compound via MeSH Substance via MeSH Hazardous Substances DB CALCIUM COMPOUNDS CALCIUM, ELEMENTAL

 Previous Article  |  Next Article 

The Journal of Neuroscience, October 1, 2002, 22(19):8379-8390

Group I Metabotropic Glutamate Receptor Signaling via Gq/G11 Secures the Induction of Long-Term Potentiation in the Hippocampal Area CA1

Masami Miura¹, Masahiko Watanabe², Stefan Offermanns³, Melvin I. Simon⁴, and Masanobu Kano¹

¹ Department of Physiology, Kanazawa University School of Medicine, Takara-machi, Kanazawa 920-8640, Japan, ² Department of Anatomy, Hokkaido University School of Medicine, Sapporo 060-8635, Japan, ³ Pharmakologisches Institut, Abteilung Molekulare Pharmakologie, Universität Heidelberg, 69120 Heidelberg, Germany, and ⁴ Division of Biology, California Institute of Technology, Pasadena, California 91125

Heterotromeric G-proteins of the Gq family are thought to transduce signals from group I metabotropic glutamate receptors (mGluRs) in central neurons. We investigated roles of this cascade in hippocampal long-term potentiation (LTP) by using null-mutant mice lacking the  subunit of Gq (Gq) or G11 (G11). We found no obvious abnormalities in the morphology, layer structure, expression of NMDA receptors, and basic parameters of excitatory synaptic transmission in the hippocampus of Gq mutant mice. We used theta burst stimulation (TBS) (3-10 burst trains at 5 Hz; each train consisted of five stimuli at 100 Hz) to induce LTP at Schaffer collateral to CA1 pyramidal cell synapses. Conventional TBS with 10 burst trains induced robust LTP in wild-type, Gq mutant, and G11 mutant mice. Weak TBS with three burst trains consistently induced LTP in wild-type mice. In contrast, the same weak TBS was insufficient to induce LTP in Gq and G11 mutant mice. In wild-type mice, the LTP by weak TBS was abolished by inhibiting group I mGluR or protein kinase C (PKC) but not by blocking muscarinic acetylcholine receptors. Prior activation of group I mGluR by an agonist significantly enhanced the LTP by weak TBS in wild-type mice. However, this priming effect was absent in Gq mutant mice. These results indicate that the signaling from group I mGluR to PKC involving Gq/G11 does not constitute the main pathway for LTP, but it secures LTP induction by lowering its threshold in the hippocampal area CA1.

Key words: long-term potentiation; hippocampus; metabotropic glutamate receptor; G-protein; protein kinase C; mouse

---

Copyright © 2002 Society for Neuroscience  0270-6474/02/22198379-12$05.00/0

This article has been cited by other articles:

				M. Miura, S. Saino-Saito, M. Masuda, K. Kobayashi, and T. Aosaki Compartment-Specific Modulation of GABAergic Synaptic Transmission by {micro}-Opioid Receptor in the Mouse Striatum with Green Fluorescent Protein-Expressing Dopamine Islands J. Neurosci., September 5, 2007; 27(36): 9721 - 9728. [Abstract] [Full Text] [PDF]

				P. J. Zhu and D. M. Lovinger Persistent Synaptic Activity Produces Long-Lasting Enhancement of Endocannabinoid Modulation and Alters Long-Term Synaptic Plasticity J Neurophysiol, June 1, 2007; 97(6): 4386 - 4389. [Abstract] [Full Text] [PDF]

				N. S. Desai, T. M. Casimiro, S. M. Gruber, and P. W. Vanderklish Early Postnatal Plasticity in Neocortex of Fmr1 Knockout Mice J Neurophysiol, October 1, 2006; 96(4): 1734 - 1745. [Abstract] [Full Text] [PDF]

				S. Y. Grooms, K.-M. Noh, R. Regis, G. J. Bassell, M. K. Bryan, R. C. Carroll, and R. S. Zukin Activity bidirectionally regulates AMPA receptor mRNA abundance in dendrites of hippocampal neurons. J. Neurosci., August 9, 2006; 26(32): 8339 - 8351. [Abstract] [Full Text] [PDF]

				N. Wettschureck, M. van der Stelt, H. Tsubokawa, H. Krestel, A. Moers, S. Petrosino, G. Schutz, V. Di Marzo, and S. Offermanns Forebrain-Specific Inactivation of Gq/G11 Family G Proteins Results in Age-Dependent Epilepsy and Impaired Endocannabinoid Formation Mol. Cell. Biol., August 1, 2006; 26(15): 5888 - 5894. [Abstract] [Full Text] [PDF]

				F. Codazzi, A. Di Cesare, N. Chiulli, A. Albanese, T. Meyer, D. Zacchetti, and F. Grohovaz Synergistic control of protein kinase Cgamma activity by ionotropic and metabotropic glutamate receptor inputs in hippocampal neurons. J. Neurosci., March 29, 2006; 26(13): 3404 - 3411. [Abstract] [Full Text] [PDF]

				N. Wettschureck and S. Offermanns Mammalian G Proteins and Their Cell Type Specific Functions Physiol Rev, October 1, 2005; 85(4): 1159 - 1204. [Abstract] [Full Text] [PDF]

				S.-R. Chen and H.-L. Pan Distinct Roles of Group III Metabotropic Glutamate Receptors in Control of Nociception and Dorsal Horn Neurons in Normal and Nerve-Injured Rats J. Pharmacol. Exp. Ther., January 1, 2005; 312(1): 120 - 126. [Abstract] [Full Text] [PDF]

				J. Hartmann, R. Blum, Y. Kovalchuk, H. Adelsberger, R. Kuner, G. M. Durand, M. Miyata, M. Kano, S. Offermanns, and A. Konnerth Distinct Roles of G{alpha}q and G{alpha}11 for Purkinje Cell Signaling and Motor Behavior J. Neurosci., June 2, 2004; 24(22): 5119 - 5130. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help