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The Journal of Neuroscience, October 1, 2002, 22(19):8739-8747

Nitric Oxide/cGMP-Mediated Protein Kinase A Activation in the Antennal Lobes Plays an Important Role in Appetitive Reflex Habituation in the Honeybee

Uli Müller¹ and Herbert Hildebrandt²

¹ Institut für Biologie der Freien Universität Berlin, Neurobiologie, 14195 Berlin, Germany, and ² Institut für Zoologie der Universität Stuttgart-Hohenheim, 70593 Stuttgart, Germany

Habituation, a form of non-associative learning, is observed throughout the animal kingdom. However, in contrast to associative learning, little is known about the underlying molecular mechanisms. Using the appetitive proboscis extension reflex in honeybees, we show that the cAMP-dependent protein kinase A (PKA) in the antennal lobe (AL) is implicated in the graded decline of behavioral response during habituation. Repeated stimulation leads to a slow and gradual increase in PKA activity superimposed on a fast transient PKA activation induced by each stimulus. These temporally distinct components of PKA activation are pharmacologically dissectible and are restricted to the AL on the stimulated side. Whereas the transient PKA activation induced by each stimulus requires monoaminergic transmission, the slow component of PKA activation is mediated by the nitric oxide (NO)/cGMP system. Local manipulation of the slow component of PKA activation in single ALs specifically interferes with the dynamic of habituation on the corresponding side. Our results provide strong evidence that NO/cGMP-mediated PKA activation in each AL contributes to temporal signal integration during habituation. Dishabituation by a sensory stimulus or spontaneous recovery from habituation does not require the PKA cascade. This provides evidence that the mechanisms underlying dishabituation and spontaneous recovery differ from those underlying temporal signal integration during habituation of the proboscis extension response.

Key words: antennal lobes; cAMP-dependent protein kinase; dishabituation; habituation; insect; plasticity; recovery from habituation; second messenger

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Copyright © 2002 Society for Neuroscience  0270-6474/02/22198739-09$05.00/0

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