QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (32) Citing Articles via Google Scholar Google Scholar Articles by White, A. R. Articles by Cappai, R. Search for Related Content PubMed PubMed Citation Articles by White, A. R. Articles by Cappai, R.

 Previous Article  |  Next Article 

The Journal of Neuroscience, January 15, 2002, 22(2):365-376

Contrasting, Species-Dependent Modulation of Copper-Mediated Neurotoxicity by the Alzheimer's Disease Amyloid Precursor Protein

Anthony R. White¹, Gerd Multhaup², Denise Galatis¹, William J. McKinstry³, Michael W. Parker³, Rüdiger Pipkorn⁴, Konrad Beyreuther², Colin L. Masters¹, and Roberto Cappai¹

¹ Department of Pathology, The University of Melbourne, Victoria 3010, and The Mental Health Research Institute, Parkville, Victoria 3052, Australia, ² Center for Molecular Biology, The University of Heidelberg, Heidelberg D-69120, Germany, ³ The Biota Structural Biology Laboratory, St. Vincent's Institute of Medical Research, Fitzroy, Victoria 3065, Australia, and ⁴ German Cancer Research Center, Heidelberg D-69120, Germany

The amyloid precursor protein (APP) of Alzheimer's disease (AD) has a copper binding domain (CuBD) located in the N-terminal cysteine-rich region that can strongly bind copper(II) and reduce it to Cu(I) in vitro. The CuBD sequence is similar among the APP family paralogs [amyloid precursor-like proteins (APLP1 and APLP2)] and its orthologs (including Drosophila melanogaster, Xenopus laevis, and Caenorhabditis elegans), suggesting an overall conservation in its function or activity. The APP CuBD is involved in modulating Cu homeostasis and amyloid  peptide production. In this paper, we demonstrate for the first time that Cu-metallated full-length APP ectodomain induces neuronal cell death in vitro. APP Cu neurotoxicity can be induced directly or potentiated through Cu(I)-mediated oxidation of low-density lipoprotein, a finding that may have important implications for the role of lipoproteins and membrane cholesterol composition in AD. Cu toxicity induced by human APP, Xenopus APP, and APLP2 CuBDs is dependent on conservation of histidine residues at positions corresponding to 147 and 151 of human APP. Intriguingly, APP orthologs with different amino acid residues at these positions had dramatically altered Cu phenotypes. The corresponding C. elegans APL-1 CuBD, which has tyrosine and lysine residues at positions 147 and 151, respectively, strongly protected against Cu-mediated lipid peroxidation and neurotoxicity in vitro. Replacement of histidines 147 and 151 with tyrosine and lysine residues conferred this neuroprotective Cu phenotype to human APP, APLP2, and Xenopus APP CuBD peptides. Moreover, we show that the toxic and protective CuBD phenotypes are associated with differences in Cu binding and reduction. These studies identify a significant evolutionary change in the function of the CuBD in modulating Cu metabolism. Our findings also suggest that targeting of inhibitors to histidine residues at positions 147 and 151 of APP could significantly alter the oxidative potential of APP.

Key words: oxidative stress; neurodegeneration; transition metals; Caenorhabditis elegans; cell culture; lipoprotein

---

Copyright © 2002 Society for Neuroscience  0270-6474/02/222365-12$05.00/0

This article has been cited by other articles:

				G. J. Brewer Iron and Copper Toxicity in Diseases of Aging, Particularly Atherosclerosis and Alzheimer's Disease Experimental Biology and Medicine, February 1, 2007; 232(2): 323 - 335. [Abstract] [Full Text] [PDF]

				R. Cappai, F. Cheng, G. D. Ciccotosto, B. E. Needham, C. L. Masters, G. Multhaup, L.-A. Fransson, and K. Mani The Amyloid Precursor Protein (APP) of Alzheimer Disease and Its Paralog, APLP2, Modulate the Cu/Zn-Nitric Oxide-catalyzed Degradation of Glypican-1 Heparan Sulfate in Vivo J. Biol. Chem., April 8, 2005; 280(14): 13913 - 13920. [Abstract] [Full Text] [PDF]

				C. Treiber, A. Simons, M. Strauss, M. Hafner, R. Cappai, T. A. Bayer, and G. Multhaup Clioquinol Mediates Copper Uptake and Counteracts Copper Efflux Activities of the Amyloid Precursor Protein of Alzheimer's Disease J. Biol. Chem., December 10, 2004; 279(50): 51958 - 51964. [Abstract] [Full Text] [PDF]

				T. A. Bayer, S. Schafer, A. Simons, A. Kemmling, T. Kamer, R. Tepest, A. Eckert, K. Schussel, O. Eikenberg, C. Sturchler-Pierrat, et al. Dietary Cu stabilizes brain superoxide dismutase 1 activity and reduces amyloid A{beta} production in APP23 transgenic mice PNAS, November 25, 2003; 100(24): 14187 - 14192. [Abstract] [Full Text] [PDF]

				Correction for Sparks et al., Trace amounts of copper in water induce {beta}-amyloid plaques and learning deficits in a rabbit model of Alzheimer's disease, PNAS 2003 100:11065-11069 PNAS, September 30, 2003; 100(20): 11816 - 11816. [Full Text] [PDF]

				D. L. Sparks and B. G. Schreurs Trace amounts of copper in water induce {beta}-amyloid plaques and learning deficits in a rabbit model of Alzheimer's disease PNAS, September 16, 2003; 100(19): 11065 - 11069. [Abstract] [Full Text] [PDF]

				K. J. Barnham, W. J. McKinstry, G. Multhaup, D. Galatis, C. J. Morton, C. C. Curtain, N. A. Williamson, A. R. White, M. G. Hinds, R. S. Norton, et al. Structure of the Alzheimer's Disease Amyloid Precursor Protein Copper Binding Domain. A REGULATOR OF NEURONAL COPPER HOMEOSTASIS J. Biol. Chem., May 2, 2003; 278(19): 17401 - 17407. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help