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The Journal of Neuroscience, November 15, 2002, 22(22):9661-9667
Inverse Relationship between Release Probability and Readily Releasable Vesicles in Depressing and Facilitating Synapses
Andrew G. Millar, Haymo Bradacs, Milton P. Charlton, and Harold L. Atwood Department of Physiology, University of Toronto, Toronto, Ontario, Canada M5S 1A8
We tested the hypothesis that the probability of vesicular exocytosis at synapses is positively correlated with the pools of readily releasable synaptic vesicles, as shown for mammalian neurons grown in tissue culture. We compared synapses of two identified glutamatergic neurons: phasic (high-output, depressing) and tonic (low-output, facilitating) crustacean motor neurons, which differ 100- to 1000-fold in quantal content. Estimates of vesicles available for exocytosis were made from depletion during forced release and from electron microscopic observation of vesicles docked at synaptic membranes near active zones. Both measurements showed a significantly larger pool of readily releasable vesicles in facilitating synapses, despite their much lower quantal output during stimulation. Thus, the probability for release of docked vesicles is very much lower at facilitating synapses, and the presence of more docked vesicles does not predict higher synaptic release probability in these paired excitatory neurons.
Key words: quantal content; glutamatergic; phasic; tonic; rapid depletion; synaptic differentiation; release probability; presynaptic; exocytosis; crustacean
Copyright © 2002 Society for Neuroscience 0270-6474/02/22229661-07$05.00/0
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