 |
|||||
|
|||||
|
|||||
|
|||||
|
|||||
Previous Article | Next Article
The Journal of Neuroscience, December 15, 2002, 22(24):10643-10652
A Novel Sorting Motif in the Glutamate Transporter Excitatory Amino Acid Transporter 3 Directs Its Targeting in Madin-Darby Canine Kidney Cells and Hippocampal Neurons
Chialin Cheng1, Greta Glover2, Gary Banker2, and Susan G. Amara1 1 Howard Hughes Medical Institute and Vollum Institute and 2 Center for Research on Occupational and Environmental Toxicology, Oregon Health and Science University, Portland, Oregon 97239
The glutamate transporter excitatory amino acid transporter 3 (EAAT3) is polarized to the apical surface in epithelial cells and localized to the dendritic compartment in hippocampal neurons, where it is clustered adjacent to postsynaptic sites. In this study, we analyzed the sequences in EAAT3 that are responsible for its polarized localization in Madin-Darby canine kidney (MDCK) cells and neurons. Confocal microscopy and cell surface biotinylation assays demonstrated that deletion of the EAAT3 C terminus or replacement of the C terminus of EAAT3 with the analogous region in EAAT1 eliminated apical localization in MDCK cells. The C terminus of EAAT3 was sufficient to redirect the basolateral-preferring EAAT1 and the nonpolarized EAAT2 to the apical surface. Using alanine substitution mutants, we identified a short peptide motif in the cytoplasmic C-terminal region of EAAT3 that directs its apical localization in MDCK cells. Mutation of this sequence also impairs dendritic targeting of EAAT3 in hippocampal neurons but does not interfere with the clustering of EAAT3 on dendritic spines and filopodia. These data provide the first evidence that an identical cytoplasmic motif can direct apical targeting in epithelia and somatodendritic targeting in neurons. Moreover, our results demonstrate that the two fundamental features of the localization of EAAT3 in neurons, its restriction to the somatodendritic domain and its clustering near postsynaptic sites, are mediated by distinct molecular mechanisms.
Key words: dendritic sorting signal; apical sorting signal; glutamate transporter; polarized trafficking; EAAT3; excitatory amino acid carrier
Copyright © 2002 Society for Neuroscience 0270-6474/02/222410643-10$05.00/0
This article has been cited by other articles:
V. Padovano, S. Massari, S. Mazzucchelli, and G. Pietrini
PKC induces internalization and retention of the EAAC1 glutamate transporter in recycling endosomes of MDCK cells
Am J Physiol Cell Physiol, October 1, 2009; 297(4): C835 - C844.
[Abstract] [Full Text] [PDF]
A. G.H. Janssen, U. Scholl, C. Domeyer, D. Nothmann, A. Leinenweber, and C. Fahlke
Disease-Causing Dysfunctions of Barttin in Bartter Syndrome Type IV
J. Am. Soc. Nephrol., January 1, 2009; 20(1): 145 - 153.
[Abstract] [Full Text] [PDF]
R. Fairless, H. Masius, A. Rohlmann, K. Heupel, M. Ahmad, C. Reissner, T. Dresbach, and M. Missler
Polarized Targeting of Neurexins to Synapses Is Regulated by their C-Terminal Sequences
J. Neurosci., November 26, 2008; 28(48): 12969 - 12981.
[Abstract] [Full Text] [PDF]
M. Bisbal, C. Conde, M. Donoso, F. Bollati, J. Sesma, S. Quiroga, A. Diaz Anel, V. Malhotra, M. P. Marzolo, and A. Caceres
Protein Kinase D Regulates Trafficking of Dendritic Membrane Proteins in Developing Neurons
J. Neurosci., September 10, 2008; 28(37): 9297 - 9308.
[Abstract] [Full Text] [PDF]
A. M. Ruggiero, Y. Liu, S. Vidensky, S. Maier, E. Jung, H. Farhan, M. B. Robinson, H. H. Sitte, and J. D. Rothstein
The Endoplasmic Reticulum Exit of Glutamate Transporter Is Regulated by the Inducible Mammalian Yip6b/GTRAP3-18 Protein
J. Biol. Chem., March 7, 2008; 283(10): 6175 - 6183.
[Abstract] [Full Text] [PDF]
V. S. Subramanian, J. S. Marchant, and H. M. Said
Apical membrane targeting and trafficking of the human proton-coupled transporter in polarized epithelia
Am J Physiol Cell Physiol, January 1, 2008; 294(1): C233 - C240.
[Abstract] [Full Text] [PDF]
R. S. Chmelar and N. M. Nathanson
Identification of a Novel Apical Sorting Motif and Mechanism of Targeting of the M2 Muscarinic Acetylcholine Receptor
J. Biol. Chem., November 17, 2006; 281(46): 35381 - 35396.
[Abstract] [Full Text] [PDF]
J. Xu, Y. Zhu, and S. F. Heinemann
Identification of sequence motifs that target neuronal nicotinic receptors to dendrites and axons.
J. Neurosci., September 20, 2006; 26(38): 9780 - 9793.
[Abstract] [Full Text] [PDF]
S. S. Das and G. A. Banker
The Role of Protein Interaction Motifs in Regulating the Polarity and Clustering of the Metabotropic Glutamate Receptor mGluR1a
J. Neurosci., August 2, 2006; 26(31): 8115 - 8125.
[Abstract] [Full Text] [PDF]
J. K. Hill, D. E. Williams, M. LeMasurier, R. A. Dumont, E. E. Strehler, and P. G. Gillespie
Splice-site A choice targets plasma-membrane Ca2+-ATPase isoform 2 to hair bundles.
J. Neurosci., June 7, 2006; 26(23): 6172 - 6180.
[Abstract] [Full Text] [PDF]
A. L. Sheldon, M. I. Gonzalez, and M. B. Robinson
A Carboxyl-terminal Determinant of the Neuronal Glutamate Transporter, EAAC1, Is Required for Platelet-derived Growth Factor-dependent Trafficking
J. Biol. Chem., February 24, 2006; 281(8): 4876 - 4886.
[Abstract] [Full Text] [PDF]
V. S. Subramanian, J. S. Marchant, and H. M. Said
Targeting and Trafficking of the Human Thiamine Transporter-2 in Epithelial Cells
J. Biol. Chem., February 24, 2006; 281(8): 5233 - 5245.
[Abstract] [Full Text] [PDF]
P.-J. Chu, J. F. Rivera, and D. B. Arnold
A Role for Kif17 in Transport of Kv4.2
J. Biol. Chem., January 6, 2006; 281(1): 365 - 373.
[Abstract] [Full Text] [PDF]
T. T. Solbu, J.-L. Boulland, W. Zahid, M. K. Lyamouri Bredahl, M. Amiry-Moghaddam, J. Storm-Mathisen, B. A. Roberg, and F. A. Chaudhry
Induction and Targeting of the Glutamine Transporter SN1 to the Basolateral Membranes of Cortical Kidney Tubule Cells during Chronic Metabolic Acidosis Suggest a Role in pH Regulation
J. Am. Soc. Nephrol., April 1, 2005; 16(4): 869 - 877.
[Abstract] [Full Text] [PDF]
H. Ohnishi, Y. Kaneko, H. Okazawa, M. Miyashita, R. Sato, A. Hayashi, K. Tada, S. Nagata, M. Takahashi, and T. Matozaki
Differential Localization of Src Homology 2 Domain-Containing Protein Tyrosine Phosphatase Substrate-1 and CD47 and Its Molecular Mechanisms in Cultured Hippocampal Neurons
J. Neurosci., March 9, 2005; 25(10): 2702 - 2711.
[Abstract] [Full Text] [PDF]
C. Vanoni, S. Massari, M. Losa, P. Carrega, C. Perego, L. Conforti, and G. Pietrini
Increased internalisation and degradation of GLT-1 glial glutamate transporter in a cell model for familial amyotrophic lateral sclerosis (ALS)
J. Cell Sci., October 15, 2004; 117(22): 5417 - 5426.
[Abstract] [Full Text] [PDF]
M. E. R. Butchbach, G. Tian, H. Guo, and C.-l. G. Lin
Association of Excitatory Amino Acid Transporters, Especially EAAT2, with Cholesterol-rich Lipid Raft Microdomains: IMPORTANCE FOR EXCITATORY AMINO ACID TRANSPORTER LOCALIZATION AND FUNCTION
J. Biol. Chem., August 13, 2004; 279(33): 34388 - 34396.
[Abstract] [Full Text] [PDF]
V. S. Subramanian, J. S. Marchant, M. J. Boulware, and H. M. Said
A C-terminal Region Dictates the Apical Plasma Membrane Targeting of the Human Sodium-dependent Vitamin C Transporter-1 in Polarized Epithelia
J. Biol. Chem., June 25, 2004; 279(26): 27719 - 27728.
[Abstract] [Full Text] [PDF]
J. C. de Jong, P. H.G.M. Willems, L. P.W.J. van den Heuvel, N. V.A.M. Knoers, and R. J.M. Bindels
Functional Expression of the Human Thiazide-Sensitive NaCl Cotransporter in Madin-Darby Canine Kidney Cells
J. Am. Soc. Nephrol., October 1, 2003; 14(10): 2428 - 2435.
[Abstract] [Full Text] [PDF]
D. Wisco, E. D. Anderson, M. C. Chang, C. Norden, T. Boiko, H. Folsch, and B. Winckler
Uncovering multiple axonal targeting pathways in hippocampal neurons
J. Cell Biol., September 29, 2003; 162(7): 1317 - 1328.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|