 |
 Previous Article
The Journal of Neuroscience, December 15, 2002, 22(24):11065-11079
Electroconvulsive Seizure Behavior in Drosophila:
Analysis of the Physiological Repertoire Underlying a Stereotyped
Action Pattern in Bang-Sensitive Mutants
Jisue
Lee and
Chun-Fang
Wu
Department of Biological Sciences, University of Iowa, Iowa City,
Iowa 52242
Drosophila bang-sensitive mutants display a
remarkable stereotyped behavioral sequence during mechanical
disturbances. This seizure repertoire consists of initial and delayed
bouts of spasm interposed with paralysis and followed by recovery of
activity and a period of refractoriness to further stimulation.
Electroconvulsive stimuli across the brain induced a similar seizure
behavior in tethered flies, in which corresponding electrophysiological
events could be readily recorded in indirect flight muscles [dorsal
longitudinal muscles (DLMs)] of the giant fiber (GF) pathway. The DLM
physiological repertoire consisted of initial and delayed discharges
(IDs and DDs), a response failure and recovery, followed by a
refractory period. Interestingly, wild-type flies also displayed the
same electroconvulsive repertoire, albeit inducible only at higher stimulus intensities and with briefer expression. The DLM repertoire presumably originated from activities of distinct neural circuits subserving normal function and reflected the general sequence of
excitation and depression of the nervous system as a whole, as shown by
simultaneous recordings along the different body axes. The well
characterized GF pathway facilitated localization of circuits
responsible for response failure and ID and DD motor patterns by
surgical manipulations, recording-stimulating site analysis, and
genetic mosaic studies. A flight pattern generator is most likely the
major contributor to shaping the DD pattern, with modifications by
active integration of individual motor neurons and associated
interneurons. The robust electroconvulsive repertoire of DLMs provides
a convenient window for further genetic analysis of the interacting
neural mechanisms underlying a stereotyped action pattern in
Drosophila, which shows striking parallels with aspects
of seizure in mammalian species.
Key words:
electroconvulsive spike discharge; seizure
susceptibility; refractory period; gynandromorph mosaics; giant fiber
pathway; bas bss
Copyright © 2002 Society for Neuroscience 0270-6474/02/222411065-15$05.00/0
This article has been cited by other articles:
|
|
|
|
 
H. Ruan and C.-F. Wu
Social interaction-mediated lifespan extension of Drosophila Cu/Zn superoxide dismutase mutants
PNAS,
May 27, 2008;
105(21):
7506 - 7510.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Fergestad, L. Olson, K. P. Patel, R. Miller, M. J. Palladino, and B. Ganetzky
Neuropathology in Drosophila Mutants With Increased Seizure Susceptibility
Genetics,
February 1, 2008;
178(2):
947 - 956.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Lee and C.-F. Wu
Genetic Modifications of Seizure Susceptibility and Expression by Altered Excitability in Drosophila Na+ and K+ Channel Mutants
J Neurophysiol,
November 1, 2006;
96(5):
2465 - 2478.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. S. Hekmat-Scafe, M. Y. Lundy, R. Ranga, and M. A. Tanouye
Mutations in the K+/Cl- Cotransporter Gene kazachoc (kcc) Increase Seizure Susceptibility in Drosophila.
J. Neurosci.,
August 30, 2006;
26(35):
8943 - 8954.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Banerjee, R. Joshi, G. Venkiteswaran, N. Agrawal, S. Srikanth, F. Alam, and G. Hasan
Compensation of inositol 1,4,5-trisphosphate receptor function by altering sarco-endoplasmic reticulum calcium ATPase activity in the Drosophila flight circuit.
J. Neurosci.,
August 9, 2006;
26(32):
8278 - 8288.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Fergestad, B. Bostwick, and B. Ganetzky
Metabolic Disruption in Drosophila Bang-Sensitive Seizure Mutants
Genetics,
July 1, 2006;
173(3):
1357 - 1364.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Song and M. A. Tanouye
Seizure Suppression by shakB2, a Gap Junction Mutation in Drosophila
J Neurophysiol,
February 1, 2006;
95(2):
627 - 635.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Banerjee, J. Lee, K. Venkatesh, C.-F. Wu, and G. Hasan
Loss of Flight and Associated Neuronal Rhythmicity in Inositol 1,4,5-Trisphosphate Receptor Mutants of Drosophila
J. Neurosci.,
September 8, 2004;
24(36):
7869 - 7878.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
